Poli (p-Aminobenzen Sülfonik Asit) Modifiye Sensöründe Askorbik Asit Varlığında Epinefrinin Elektrokimyasal Tayini

Year 2018, , 1025 - 1035, 24.12.2018

Şevket Zişan Bilge Yağcı Ebru Kuyumcu Savan Gamze Erdoğdu

https://doi.org/10.17776/csj.426874

Abstract

Poli (p-aminobenzen
sülfonik asit, ABSA) modifiye sensör ile askorbik asit varlığında epinefrinin
(EP) hızlı, güvenilir ve duyarlı bir şekilde seçici ve eş zamanlı voltametrik
tayini gerçekleştirildi. Camsı karbon elektrot, ABSA ile 0.1 M KCl çözeltisi
içerisinde çevrimli voltametri tekniğiyle başarılı bir şekilde modifiye edildi.
Polimerik ince film ile modifiye edilen sensör, EP ve askorbik asitin (AA)
yükseltgenmesine karşı mükemmel elektro-katalitik etki göstermiştir. Sonuçlar,
her iki türün oksidasyon potansiyeli ve oksidasyon pik akım yanıtlarının önemli
derecede iyileştiğini göstermiştir. Modifiye sensör, optimum koşullar altında
EP tayinine karşı 5.0–53.2 µM derişim aralığında 50 nM tayin sınırı ile
mükemmel cevap göstermiştir. Farmasötik preparatta ve kan serumunda
gerçekleştirilen gerçek numune analizlerinde geri kazanım değerleri % 98.4 -
77.3 di. Elde edilen sonuçlar, modifiye sensörün AA varlığında EP’in
saptanmasında uygulanabileceğini göstermiştir. Önerilen sensörün, yüksek
seçicilik, tekrarlanabilirlik, tekrarüretilebilirlik ve uzun-süreli kararlılık
özelliklerinden ve farmasötik ve biyolojik örneklerde elde edilen yüksek geri
kazanım değerlerinden dolayı rutin analizlerde uygulanabilecek olması umut
vericidir.

Keywords

Epinefrin, p-aminobenzen sülfonik asit, sensör, voltametri

Electrochemical Detection of Epinephrine in The Presence of Ascorbic Acid at Poly (p-Aminobenzene Sulfonic Acid) Modified Sensor

Abstract

In this study, a rapid, reliable, selective and sensitive simultaneous
voltammetric determination of Epinephrine (EP) in the presence of ascorbic acid
at poly (p-aminobenzene sulphonic
acid, ABSA) modified sensor was aimed. The glassy carbon electrode was
successfully modified with ABSA in 0.1 M KCl solution by the cycling
voltammetry technique. The sensor, modified with a polymeric thin film, showed
excellent electrocatalytic activity against the oxidation of EP and ascorbic
acid (AA). The results showed that the oxidation potential and current
responses of EP and AA improved significantly. The modified sensor showed
excellent response with limit of detection as 50 nM in the determination of EP
at the 5.0 - 53.2 μM concentration range under optimum conditions. In real
sample analyzes performed in pharmaceutical preparation and blood serum,
recovery values were 77.3%-98.4%. The results obtained show that the modified
sensor can be applied to the detection of EP in the presence of AA. The proposed
sensor is promising for routine analysis because of its high selectivity,
reproducibility, reproducibility and long-term stability characteristics and
high recovery values obtained in pharmaceutical and biological samples.

Keywords

Epinephrine, p-aminobenzene sulfonic acid, sensor, voltammetry, voltammetry

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