Memantine activates LKB1-AMPK pathway in breast carcinoma cells

Elif Burcu Bali

doi:10.17776/csj.768907

EN

Memantine activates LKB1-AMPK pathway in breast carcinoma cells

Abstract

Drug repositioning that is a screening of presently approved drugs for already unknown indications is therapeutically necessary and influential for drug discovery. In this study, it was aimed to research whether memantine as a repositioned drug can activate the LKB1-AMPK pathway in breast carcinoma cells by triggering tumor suppressor genes LKB1, AMPK, its downstream targets 40S ribosomal S6 kinases (S6K1 and S6K2), and eukaryotic initiation factor 4E-binding protein 4E-BP1. It was also evaluated its apoptotic effect by detecting the gene expressions of Caspase 7 and NOXA. Thus, MCF-7 cells were treated with 250 µM memantine for 48 h, and its cytotoxic effect was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. AMPKα1, AMPKα2, S6K1, S6K2, 4E-BP1, Caspase 7 and NOXA gene expression levels were measured by quantitative real-time polymerase chain reaction. The results clearly revealed that memantine inhibited MCF-7 cell proliferation and activated the LKB1-AMPK pathway by reducing S6K1, S6K2, and 4EBP1 gene expressions. Memantine also augmented the gene expressions of Caspase 7 and NOXA. The findings reveal a molecular mechanism for the first time that may contribute to the anti-cancer effect of memantine to prevent or treat breast cancer. But further research should be performed to better understand its anti-cancer action.

Keywords

Memantine, breast carcinoma, LKB1-AMPK pathway, real-time polymerase chain reaction, Caspase 7

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Elif Burcu Bali ^*
0000-0002-8797-0573
Türkiye

Publication Date

December 29, 2020

Submission Date

July 13, 2020

Acceptance Date

November 3, 2020

Published in Issue

Year 2020 Volume: 41 Number: 4

DOI

https://doi.org/10.17776/csj.768907

IZ

https://izlik.org/JA24PA76RD

APA

Bali, E. B. (2020). Memantine activates LKB1-AMPK pathway in breast carcinoma cells. Cumhuriyet Science Journal, 41(4), 775-783. https://doi.org/10.17776/csj.768907

AMA

1.Bali EB. Memantine activates LKB1-AMPK pathway in breast carcinoma cells. CSJ. 2020;41(4):775-783. doi:10.17776/csj.768907

Chicago

Bali, Elif Burcu. 2020. “Memantine Activates LKB1-AMPK Pathway in Breast Carcinoma Cells”. Cumhuriyet Science Journal 41 (4): 775-83. https://doi.org/10.17776/csj.768907.

EndNote

Bali EB (December 1, 2020) Memantine activates LKB1-AMPK pathway in breast carcinoma cells. Cumhuriyet Science Journal 41 4 775–783.

IEEE

[1]E. B. Bali, “Memantine activates LKB1-AMPK pathway in breast carcinoma cells”, CSJ, vol. 41, no. 4, pp. 775–783, Dec. 2020, doi: 10.17776/csj.768907.

ISNAD

Bali, Elif Burcu. “Memantine Activates LKB1-AMPK Pathway in Breast Carcinoma Cells”. Cumhuriyet Science Journal 41/4 (December 1, 2020): 775-783. https://doi.org/10.17776/csj.768907.

JAMA

1.Bali EB. Memantine activates LKB1-AMPK pathway in breast carcinoma cells. CSJ. 2020;41:775–783.

MLA

Bali, Elif Burcu. “Memantine Activates LKB1-AMPK Pathway in Breast Carcinoma Cells”. Cumhuriyet Science Journal, vol. 41, no. 4, Dec. 2020, pp. 775-83, doi:10.17776/csj.768907.

Vancouver

1.Elif Burcu Bali. Memantine activates LKB1-AMPK pathway in breast carcinoma cells. CSJ. 2020 Dec. 1;41(4):775-83. doi:10.17776/csj.768907

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