Development of Analytical Method for Sensitive Determination of Streptozotocin based on Solid Phase Extraction
Abstract
This study is focused on developing a sensitive analytical method for Streptozotocin (STZ) molecules which is a naturally occurring alkylating antineoplastic agent. It is also known particularly toxic to the insulin-producing beta cells of the pancreas in mammals. With this study, a separation-enrichment method based on solid phase extraction was developed for low levels of STZ. The synthetized material consist of a multi-walled carbon nanotube/CuO/Cu2O was used as an adsorbent in column type SPE. Experimental variables such as pH, ionic strength, desorption solvent, and other parameters were studied and optimized step by step. Analytical merits were obtained under the optimized conditions: linear range (100.00-1500.00 ng mL-1), limit of detection (28.57 ng mL-1), relative standard deviation (RSD) % for 250 ng mL-1 of STZ (< 4.2 %, N:3), and pre-concentration factor (125). In order to test accuracy of the method, recovery tests were applied successively by using model solutions.
Keywords
Streptozotocin, Solid Phase Extraction,, Analytical Method Development
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