Bazı Cladonia Likenlerinin Bitki Patojeni Bakterileri Üzerine Etkileri ve Bakır İndirgeme Antioksidan Kapasitesi Aktiviteleri

Year 2017, Volume: 38 Supplement Issue 4, 105 - 114, 08.12.2017

Sinem Aydın Bahar Bılgın Sokmen Kadir Kınalıoglu

https://doi.org/10.17776/csj.358762

Cited By: 1

Abstract

Bakteri ve mantar gibi mikroorganizmalar bitkilerde
pek çok hastalığa yol açmaktadır. Patojenik mikroorganizmaların çoğu sıklıkla
kullanılan antibiyotiklere ve sentetik fungisitlere direnç kazanmıştır.
Likenler bir mantar, bir alg veya bir siyanobakteriyum arasındaki simbiyotik
birliklerdir. Likenler ve metabolitlerinin sentetik kimyasallara karşı umut
vaat edici alternatifler olabilecekleri gösterilmiştir. Bu çalışmanın amacı Cladonia
stellaris, Cladonia pyxidata ve Cladonia furcata likenlerinin etil asetat
ekstraktlarının antibakteriyal etkisini ortaya koymaktır. Ekstraktlar sokslet
ekstraksiyonu ile elde edilmiştir. Antibakteriyal aktivite disk difüzyon metodu
ile belirlenirken, antioksidan aktivite bakır indirgeme antioksidan kapasitesi
(CUPRAC) metodu ile belirlenmiştir. En iyi antibakteriyal aktivite C. stellaris ekstraktında (25 mm)
pozitif kontrol antibiyotiklerinden (ampisilin ve tetrasiklin) daha fazla
olarak Pseudomonas
syringae pv. syringae ve Erwinia carotovora subsp. carotovora’ya
karşı bulunmuştur. CUPRAC aktivitesi C. furcata>C.
pyxidata>C. stellaris şeklinde
artmaktadır. Liken
ekstraktlarından aktif bileşenlerin izolasyonu ve bu bileşiklerin biyolojik
aktivitelerinin belirlenmesinde daha ileri çalışmalar gerekmektedir.

Keywords

Liken, Antibiyotik, Antioksidan aktivite

Influence of Some Cladonia Lichens on Plant Pathogenic Bacteria and Copper Reducing Antioxidant Capacity Activities

Abstract

Microorganisms
such as bacteria and fungi lead many diseases in plants. A great deal of
pathogenic microorganisms has gained resistance against frequently utilized
antibiotics and synthetic fungicides. Lichens are symbiotic associations
between a fungus, an algae or a cyanobacterium. Lichens and their metabolites
are demonstrated to be encouraging alternatives to synthetic chemicals. The aim
of this study was to reveal the antibacterial effect of ethyl acetate extracts
of Cladonia stellaris, Cladonia pyxidata and Cladonia furcata lichen species.
Extracts were obtained by Soxhlet extraction. Whereas antibacterial activity
was determined by the disc diffusion method, antioxidant activity was
determined by copper reducing antioxidant capacity (CUPRAC) method. The best
antibacterial activity was found in C.
stellaris extract (25 mm), which was stronger than the positive control
antibiotics (ampicillin and tetracycline) against Pseudomonas syringae pv. syringae and Erwinia carotovora subsp. carotovora. The CUPRAC activity was increased in
the following order: C. furcata>C. pyxidata>C. stellaris. Further
studies are needed on isolation of active constituents from lichen extracts and
their biological activity studies are to be worked out.

Keywords

Lichen, Antibiotic, Antioxidant activity

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