Dermatolojik Amaçlı Sumak Özütü İçeren Organofilik Kil-Nanokompozit Sentezi ve Biyoaktivitesinin Araştırılması

Year 2024, Volume: 3 Issue: 2, 76 - 86, 26.12.2024

Gülay Gültek , Özcan Deniz

https://doi.org/10.69560/cujast.1551319

Abstract

Killerin kozmetik amaçlı kullanımı antik çağlardan beri, terapötik kullanımı ise tarih öncesinden beri var olmuştur ve günümüz kozmetik endüstrisinde dünya çapında önemli miktarda kullanılmaktadır. Birçok kozmetik ürününde genellikle aktif içerik olarak antioksidan ve antibakteriyel bileşikler kullanılmaktadır. Bu araştırmada, kozmetik formülasyonlarda yaygın olarak kullanılan kil (montmorillonit, MMT) mineralinin kullanım amaçları doğrultusunda etkinliğinin artırılması amacıyla yenilikçi bir yaklaşımla biyoaktif organofilik naokompozitlerinin hazırlanması ve biyoaktivitesinin test edilmesi amaçlanmıştır. Bu amaçla yerli kaynaklardan alınan montmorillonit kili sedimentasyon yöntemiyle saflaştırıldı. Dodesiltrimetil amonyum klorür çözeltisi kullanılarak montmorillonit kili organofilik (OMMT) hale getirildi. Şifalı bitkiler arasında bulunan ve baharat olarak kullanılan sumak (meyve kısmı) attardan alınarak kurutuldu, öğütüldü ve su, etanol ve n-hekzan ile ayrı ayrı 8 saat boyunca Soxlet ekstraksiyon düzeneğinde özütlendi. n-kekzan özütündeki n-hekzan vakum altında uzaklaştırıldı ve elde edilen katı tekrar su/etanol çözeltisi ile özütlenerek %95 verimle gıda kalitesinde (foodgrade) sumak özütü elde edildi. Su ve etanolik ekstrakların çözücüleri de vakum altında uzaklaştırıldı. OMMT içerisindeki sumak özütü miktarı %1; %3 ve %5 olacak şekilde nanokompozitleri hazırlandı ve PEG-400 ile uyumlaştırılarak biyoaktiviteleri test edildi. Biyoaktivite testleri, deri yüzeyinde üreyen gram pozitif Staphylococcus aureus ve gram negatif bakterisi Escherichia coli kullanılarak Nutrient Agar ve Violet Bile Red Agar ortamında 37oC’de yapıldı. Üç farklı bileşimde hazırlanan kil nanokompozilerin tamamı Staphylococcus aureus ve Escherichia coli bakterilerinin üremesini inhibe ederek biyoaktif özelliğe sahip olduklarını gösterdi.

Keywords

Organofilik montmorillonit, nanokompozit, biyoaktivite, dermatolojik kil, sumak özütü

Synthesis of Organophilic Clay-Nanocomposite Containing Sumac Extract for Dermatological Purposes and Investigation of Its Bioactivity

Abstract

The use of clays for cosmetic purposes has existed since ancient times, and its therapeutic use has existed since prehistory, and today's cosmetic industry uses significant amounts worldwide. Antioxidant and antibacterial compounds are generally used as active ingredients in many cosmetic products. In this study, it was aimed to prepare bioactive organophilic nanocomposites with an innovative approach in order to increase the effectiveness of clay (montmorillonite, MMT) mineral, which is widely used in cosmetic formulations, in line with its intended use and to test its bioactivity. For this purpose, montmorillonite clay obtained from domestic sources was purified by sedimentation method. Montmorillonite clay was made organophilic (OMMT) by using dodecyltrimethyl ammonium chloride solution. Sumac (fruit part), which is among the medicinal plants and used as a spice, was taken from the attar, dried, ground and extracted with water, ethanol and n-hexane separately for 8 hours in a Soxlet extraction apparatus. The n-hexane in the n-hexane extract was removed under vacuum and the obtained solid was extracted again with water/ethanol solution to obtain food grade sumac extract with 95% yield. The solvents of water and ethanolic extracts were also removed under vacuum. Nanocomposites were prepared with 1%, 3% and 5% sumac extract amount in OMMT and their bioactivity was tested by compatibilization with PEG-400. Bioactivity tests were performed on Nutrient Agar and Violet Bile Red Agar media at 37oC using gram positive Staphylococcus aureus and gram negative Escherichia coli bacteria growing on the skin surface. All of the clay nanocomposites prepared in three different compositions showed that they had bioactive properties by inhibiting the growth of Staphylococcus aureus and Escherichia coli bacteria.

Keywords

Organophilic montmorillonite, nanocomposite, bioactivity, dermatological clay, sumac extract

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