Bazı Bitki Kaynaklarının Farklı Çözgenlerde Hazırlanan Ekstrelerinin Antimikrobiyal Aktivitesinin Araştırılması

Year 2022, , 543 - 554, 30.06.2022

Burcu Sancar Beşen Leyla Eren Karahan , Pınar Parlakyiğit Cumhur Kırılmış Sakine Erdoğan

https://doi.org/10.21605/cukurovaumfd.1146592

Abstract

Bu çalışmada, aloe vera (Aloe barbadensis), aynısefa (Calendula officinalis), baldırıkara (Adiantum capillus-veneris Linn.) ve kudret narı (Momordica charantia) bitkileri gibi bazı bitki kaynaklarının farklı çözgenler kullanılarak hazırlanan ekstrelerinin antimikrobiyal aktivitesinin araştırılması amaçlanmıştır. Bu amaçla, bitkilerin n-hegzan, etanol, metanol ve distile su içerisinde maserasyon yöntemi ile ekstreleri hazırlanmış ve hazırlanan ekstrelerinin, Staphylococcus aureus ve Escherichia coli bakterilerine karşı antibakteriyel; Aspergillus niger mantarına karşı antifungal aktivitesi incelenmiştir. Çalışma sonuçları, ekstraksiyonda kullanılan bitki kaynağının ve çözgen türünün ekstrelerin antimikrobiyal aktivitesi üzerinde oldukça önemli olduğunu ortaya koymuştur.

Keywords

Antimikrobiyal aktivite, Bitki ekstresi, Aloe vera, Aynı sefa, Baldırıkara, Kudret narı

Investigation of Antimicrobial Activities of Extracts of Some Plant Sources Prepared in Different Solvents

Abstract

In the present study, it was aimed to research the antimicrobial activities of prepared extracts of some plant sources such as aloe vera (Aloe barbadensis), marigold (Calendula officinalis), maidenhair fern (Adiantum capillus-veneris Linn.) and bitter melon (Momordica charantia) by using different solvents. For this purpose, the extracts of the plants in the solvents as n-hegzan, ethanol, methanol and distilled water were prepared via maceration process. Either antibacterial activies against Staphylococcus aureus and Escherichia coli or antifungal activities against Aspergillus niger of the prepared extracts were investigated. The results demonstrated that the plant sources and solvent types used in the extraction are crucial on the antimicrobial activities of the extracts.

Keywords

Antimicrobial activity, Plant extract, Aloe vera, Marigold, Maidenhair fern, Bitter melon

References

• 1. Acet, T., Özcan, K., 2018. Aslanpençesi (Alchemilla ellenbergiana) Ekstrelerinin Antioksidan ve Antimikrobiyal Özelliklerinin Belirlenmesi. GÜFBED/GUSTIJ, 8(1), 113-121
• 2. İnce, İ., Çığırgil, N., Gümüştaş, B., Kozguş Güldü, Ö., Karaman, D., Medine, E.İ., Güler, G., Karasulu, E., 2019. Geleneksel Olarak Yara Tedavisinde Kullanılan Kudret Narı (Momordica charantia L.) Zeytinyağı Maseratı Kullanılarak Krem Formunun Geliştirilmesi ve İn Vitro Yara İyi Edici Etkisinin Araştırılması. Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 12(1), 38–48.
• 3. Çelik, E., Çelik, G.Y., 2007. Bitki Uçucu Yağların Antimikrobiyal Özellikleri. Orlab On-Line Mikrobiyoloji Dergisi, 5(2), 1-6.
• 4. Ayhancı, T., 2019. Bazı Endemik Bitkilerin Antimikrobiyal ve Antioksidan Aktivitelerinin İncelenmesi. Yüksek Lisans Tezi, Ege Üniversitesi, Fen Bilimleri Enstitüsü, Biyokimya Anabilim Dalı, İzmir, 109
• 5. Manandhar, S., Luitel, S., Dahal, R.K., 2019. In Vitro Antimicrobial Activity of Some Medicinal Plants Against Human Pathogenic Bacteria. Journal of Tropical Medicine, https://doi.org/10.1155/2019/1895340
• 6. Boudreau, M.D., Beland, F.A., 2006. An Evaluation of the Biological and Toxicological Properties of Aloe Barbadensis (Miller). Aloe Vera Journal of Environmental Science and Health Part C, 24, 103–154.
• 7. Surjushe, A., Vasani, R., Saple, D.G., 2008. Aloe Vera: A Short Review. Indian J Dermatol, 53(4), 163–166.
• 8. Okuma, C.H., Andrade, T.A.M., Caetano, G.F., Finci, L.I., Maciel, N.R., Topan, J.F., Cefali, L.C., Polizello, A.C.M., Carlo, T., Rogerio, A.P., Spadaro, A.C.C., Isaac, V.L.B., Frade, M.A.C., Rocha-Filho, P.A., 2015. Development of Lamellar Gel Phase Emulsion Containing Marigold Oil (Calendula officinalis) as a Potential Modern Wound Dressing. European Journal of Pharmaceutical Sciences, 71, 62–72.
• 9. Vinod, M., Singh, M., Pradhan, M., Iyer, S.K., Tripathi, D.K., 2012. Phytochemical Constituents and Pharmacological Activities of Betula Alba Linn.- A Review. International Journal of PharmTech Research, 4(2), 643–647.
• 10. Abbasi, A.M., Khan, M.A., Ahmad, M., Zafar, M., Jahan, S., Sultana, S., 2010. Ethnopharmacological Application of Medicinal Plants to Cure Skin Diseases and in Folk Cosmetics Among the Tribal Communities of North-west Frontier Province, Pakistan. J. Ethnopharmacol, 128, 322–335.
• 11. Al-Qura’n, S., 2009. Ethnopharmacological Survey of Wild Medicinal Plants in Showbak. Jordan, J. Ethnopharmacol. 123, 45–50.
• 12. Dehdari, S., Hajimehdipoor, H., 2018. Medicinal Properties of Adiantum Capillus-Veneris Linn. In Traditional Medicine and Modern Phytotherapy: A Review Article, Iranian Journal of Public Health, 47(2), 188–197.
• 13. Dandawate, P.R., Subramaniam, D., Padhye, S.B., Anant, S., 2016. Bitter Melon: A Panacea for Inflammation and Cancer. Chinese Journal of Natural Medicines, 14(2), 81–100.
• 14. Chipps, E.S., Jayini, R., Ando, S., Protzman, A.D., Muhi, M.Z., Mottaleb, M.A., Malkawi, A., Islam, M.R., 2012. Cytotoxicity Analysis of Active Components in Bitter Melon (Momordica charantia) Seed Extracts Using Human Embryonic Kidney and Colon Tumor Cells. Natural Product Communications, 7(9), 1203–1208.
• 15. El-Shafei, A., Shaarawy, S., Motawe, F.H., Refaei, R., 2018. Herbal Extract as an Ecofriendly Antimicrobial Finishing of Cotton Fabric. Egyptian Journal of Chemistry, 61(2), 317–327.
• 16. Selvam, R.M., Athinarayanan, G., Nanthini, A.U.R., Singh, A.J.A.R., Kalirajan, K., Selvakumar, P.M., 2015. Extraction of Natural Dyes from Curcuma longa, Trigonella Foenum Graecum and Nerium Oleander, Plants and Their Application in Antimicrobial Fabric. Industrial Crops and Products, 70, 84–90.
• 17. Çete, S., Arslan, F., Yaşar, A. 2005. Investigation of Antimicrobial Effects Against Some Microorganisms of Aloe Vera and Nerium Oleander Also Examination of the Effects on the Xanthine Oxidase Activity in Liver Tissue Treated with Cyclosporin. G.Ü. Fen Bilimleri Dergisi, 18(3), 375-380.
• 18. Kozlowska, J., Stachowiak, N., Prus, W., 2019. Stability Studies of Collagen-based Microspheres with Calendula Officinalis Flower Extract, 163, 214–219.
• 19. Parente, L.M.L., Lino Júnior, R.D.S., Tresvenzol, L.M.F., Vinaud, M.C., Paula, J.R. De, Paulo, N.M., 2012. Wound Healing and Anti-Inflammatory Effect in Animal Models of Calendula Officinalis L. growing in Brazil, Evidence-based Complementary and Alternative Medicine, 2012, 1-7.
• 20. Lindsay, S., Suksathan, P., Middleton, D.J. 2010. A New Species of Adiantum (Pteridaceae) from Northern Thailand. Thai Forest Bull. (Botany), 38, 67-69.
• 21. Singh, M., Singh, N., Khare, P.B., Rawat, A.K.S., 2008. Antimicrobial Activity of Some Important Adiantum Species Used Traditionally in Indigenous Systems of Medicine. J. Ethnopharmacol, 115, 327–329.
• 22. Baldemir, A., Ekinci, K., İlgün, S., Dalda, A., Yetişir, H., 2018. Momordica Charantia L. (Kudret narı) Meyvelerinin Toplam Fenolik Madde İçerikleri ve Antioksidan Kapasitelerinin Değerlendirilmesi. Derim, 35(1), 45-50.
• 23. Cheng, H.L., Yang, M.H., Anggriani, R., Chang, C.I., 2017. Comparison of Anti-inflammatory Activities of Structurally Similar Triterpenoids Isolated from Bitter Melon, Natural Product Communications, 12(12), 1847–1850.
• 24. Bissa, S., Bohra, A., 2011. Antibacterial Potential of Pot Marigold. Journal of Microbiology and Antimicrobials, 3(3), 51-54.
• 25. Chakraborthy, G.S., 2008. Antimicrobial Activity of the Leaf Extracts of Calendula Officinalis (Linn). Journal of Herbal Medicine and Toxicology, 2(2), 65-66.
• 26. Costa, J.G.M., Nascimento, E.M.M., Campos, A.R., Rodrigues, F.F.G., 2011. Antibacterial Activity of Momordica Charantia (Curcubitaceae) Extracts and Fractions. Journal of Basic and Clinical Pharmacy. 2(1), 45-51.
• 27. Ranjan, V., Vats, M., Gupta, N., Sardana, S., 2014. Antidiabetic Potential of Whole Plant of Adiantum Capillus Veneris Linn. in Streptozotocin Induced Diabetic Rats. International Journal of Pharmaceutical and Clinical Research, 6(4), 341-347.
• 28. Hosseinkhani, A., Falahatzadeh, M., Raoofi, E., Zarshenas, M.M., 2017. An Evidence-Based Review on Wound Healing Herbal Remedies from Reports of Traditional Persian Medicine. Journal of Evidence-based Complementary & Alternative Medicine, 22(2), 334-343.
• 29. Beşen, B.S., 2019. Production of Disposable Antibacterial Textiles Via Application of Tea Tree Oil Encapsulated into Different Wall Materials. Fibers and Polymers, 20(12), 2587-2593.
• 30. Beşen, B.S., 2020. Tea Tree Oil/Ethyl Cellulose Microcapsule Loaded Antimicrobial Textiles. AATCC Journal of Research, 7(2), 1-6.
• 31. Kavas, C., 2002. Gıda Sanayinde Starter Kültür Olarak Kullanılan Bazı Laktik Asit Bakterilerinin Antifungal Etkilerinin Araştırılması. Yüksek Lisans Tezi, Çukurova Üniversitesi, Fen Bilimleri Enstitüsü, Adana, 64.
• 32. Beşen, B.S., 2016. Ozonlanmiş Bı tkı sel Yağ İ çeren Mı krokapsüllerı n Üretı lmesı ve Tekstı l Yüzeylerı ne Aktarılması. Doktora Tezi. Kahramanmaraş Sütçü İmam Üniversitesi, Fen Bilimleri Enstitüsü, Kahramanmaraş, 183.