Apical Sealing Ability of Different Endodontic Sealers Using Glucose Penetration Test: A Standardized Methodological Approach

Year 2020, Volume: 23 Issue: 2, 79 - 87, 30.06.2020

Galvin Sim Siang Lin Nik Rozainah Nik Abdul Ghani Tahir Yusuf Noorani Aimi Kamarudin

https://doi.org/10.7126/cumudj.704107

Cited By: 9

Abstract

Objectives: To compare the apical sealing
ability of four endodontic sealers based on glucose penetration method and validate
the uses of contralateral teeth to provide a well-balanced experimental group.

Materials and methods: One-hundred-and-twenty
(sixty pair) extracted contralateral lower premolars were selected and
undergone strict radiographic protocol. Root canal anatomy of each pair contralateral
teeth was matched buccolingually and mesiodistally according to inclusion
criteria (single canal, mature apical foramen, canal type, canal width, length,
and curvature). Matched-pair contralateral teeth were then reevaluated using
CBCT and divided into right and left sides (n=60, each side). Next, all canals
were instrumented up to size 30, taper 0.06. Subsequently, teeth were
subdivided into five groups for each side and obturated with single cone gutta-percha
(GP) and various sealers: Group 1 - GP only (control); Group 2 - EndoRez; Group
3 - Sealapex; Group 4 - EndoSeal MTA and Group 5 - BioRoot RCS. All samples
were placed in an incubator at 37°C, 100% humidity for 72 hours. Four
matched-pair teeth from each group were then subjected to thermocycling for 100
cycles, 1000 cycles and 10000 cycles, respectively. After that, they were
decoronated, coated with three layers of nail varnish, and used for glucose penetration test. The
concentrations of glucose (mmol/L) were measured after 24 hours. Data analyzed
using One-way ANOVA complemented by post hoc Dunnett T3 Test and Paired sample
T-Test.

Results: EndoSeal MTA demonstrated statistically significant (p<0.05)
lowest glucose penetration followed by BioRoot RCS, Sealapex, EndoRez, and
lastly control group. Apical sealing ability decreased as the number of
thermocycles increased. No significant difference (p>0.05) was found
between matched-pair contralateral teeth.

Conclusions: Bioceramic
sealers demonstrated better sealing ability than resin and calcium hydroxide
sealers. Using matched-pair contralateral teeth provided a well-balanced
experimental group.

Keywords

EndoRez, Mineral trioxide aggregate, Root canal filling materials, Sealapex, Tricalcium silicate

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