Year 2016, Volume 37 , Issue 2, Pages 109 - 119 2016-04-20

Expression of Extracellular Matrix Proteins in Basal Membranes During Fetal Nephron Development in Mice
During Fetal Nephron Development in Mice

Beyhan GÜRCÜ [1] , İbrahim TUĞLU [2] , Sabire KARAÇALI [3]


Abstract. In this study, we investigated the distribution of laminin, collagen type IV, nidogen and fibronectine during metanephric development in fetal mouse kidney by immunohistochemistry. Stain density of basement membranes of tubules, glomerules and mesangial matrix were compared in pre-capillary, immature glomerular and mature glomerular stages of fetal kidney. All the matrix proteins were strongly stained in precapillary stage. In immature glomerular stage, a strong staining was observed for fibronectin. Staining intensity was slightly decreased for the other proteins in this stage. In mature glomerular stage, diminished staining for all proteins was observed similar to the previous stage, except fibronectin. The strongest immunoreactions were found for fibronectin and nidogen in all investigated stages. In general, there was a similar staining intensity for all glycoproteins during maturation except for laminin. It was thought that the distribution of extracellular matrix molecules plays an important role for the kidney development. Interactions amoung these molecules probably crucial on cell behavior like migration, proliferation and differentiation in normal development of the nephron.

Keywords: Fibronectine, Nidogen, Laminin, Collagen type IV, Immunohistochemistry, Fetal Kidney, Mouse

 

 

Özet. Çalışmamızda, fötal fare böbreği metanefrik gelişimde laminin, kollagen tip IV, nidogen ve fibronektin dağılımını imunohistokimyasal olarak inceledik. Tübül, glomerul ve mesangial matriks bazal membran boyanma yoğunluğu, fötal böbreğin pre-kapiller, olgunlaşmamış glomerül ve olgunlaşmış glomerül safhalarında karşılaştırıldı. Matriks proteinlerinin hepsi pre-kapiller safhada koyu boyandı. Olgunlaşmamış glomerül safhasında, fibronektin kuvvetli boyanırken diğer proteinlerin boyanma yoğunluğu azaldı. Olgunlaşmış glomerül safhasında ise fibronektin hariç diğer proteinlerin boyanma yoğunluğu olgunlaşmamış glomerül safhası ile benzerdi. İncelenen tüm safhalarda en yoğun immunreaksiyon nidogen ve fibronektinde bulundu. Genellikle, laminin hariç olgunlaşma boyunca tüm glikoproteinlerin boyanma yoğunluğu benzerdi. Bu, hücreler arası madde proteinlerinin böbrek gelişiminde önemli bir rol oynadığını düşündürmektedir. Muhtemelen bu moleküller arasındaki etkileşimler, normal bir nefron gelişiminde göç, çoğalma ve farklılaşma gibi hücre davranışlarında oldukça önemlidir.

Anahtar Kelimeler: Fibronektin, Nidogen, Laminin, Kollajen tip IV, İmmunohistokimya, Fötal böbrek, Fare

Çalışmamızda, fötal fare böbreği metanefrik gelişimde laminin, kollagen tip IV, nidogen ve fibronektin dağılımını imunohistokimyasal olarak inceledik. Tübül, glomerul ve mesangial matriks bazal membran boyanma yoğunluğu, fötal böbreğin pre- kapiller, olgunlaşmamış glomerül ve olgunlaşmış glomerül safhalarında karşılaştırıldı. Matriks proteinlerinin hepsi pre-kapiller safhada koyu boyandı. Olgunlaşmamış glomerül safhasında, fibronektin kuvvetli boyanırken diğer proteinlerin boyanma yoğunluğu azaldı. Olgunlaşmış glomerül safhasında ise fibronektin hariç diğer proteinlerin boyanma yoğunluğu olgunlaşmamış glomerül safhası ile benzerdi. İncelenen tüm safhalarda en yoğun immunreaksiyon nidogen ve fibronektinde bulundu. Genellikle, laminin hariç olgunlaşma boyunca tüm glikoproteinlerin boyanma yoğunluğu benzerdi. Bu, hücreler arası madde proteinlerinin böbrek gelişiminde önemli bir rol oynadığını düşündürmektedir. Muhtemelen bu moleküller arasındaki etkileşimler, normal bir nefron gelişiminde göç, çoğalma ve farklılaşma gibi hücre davranışlarında oldukça önemlidir
Fibronektin, Nidogen, Laminin, Kollajen tip IV, İmmunohistokimya, Fötal böbrek, Fare
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Journal Section Natural Sciences Research Article
Authors

Author: Beyhan GÜRCÜ

Author: İbrahim TUĞLU

Author: Sabire KARAÇALI

Dates

Publication Date : April 20, 2016

Bibtex @ { cumuscij264103, journal = {Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi}, issn = {1300-1949}, eissn = {1300-1949}, address = {}, publisher = {Cumhuriyet University}, year = {2016}, volume = {37}, pages = {109 - 119}, doi = {10.17776/csj.10236}, title = {Expression of Extracellular Matrix Proteins in Basal Membranes During Fetal Nephron Development in Mice}, key = {cite}, author = {GÜRCÜ, Beyhan and TUĞLU, İbrahim and KARAÇALI, Sabire} }
APA GÜRCÜ, B , TUĞLU, İ , KARAÇALI, S . (2016). Expression of Extracellular Matrix Proteins in Basal Membranes During Fetal Nephron Development in Mice. Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi , 37 (2) , 109-119 . DOI: 10.17776/csj.10236
MLA GÜRCÜ, B , TUĞLU, İ , KARAÇALI, S . "Expression of Extracellular Matrix Proteins in Basal Membranes During Fetal Nephron Development in Mice". Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi 37 (2016 ): 109-119 <https://dergipark.org.tr/en/pub/cumuscij/issue/25013/264103>
Chicago GÜRCÜ, B , TUĞLU, İ , KARAÇALI, S . "Expression of Extracellular Matrix Proteins in Basal Membranes During Fetal Nephron Development in Mice". Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi 37 (2016 ): 109-119
RIS TY - JOUR T1 - Expression of Extracellular Matrix Proteins in Basal Membranes During Fetal Nephron Development in Mice AU - Beyhan GÜRCÜ , İbrahim TUĞLU , Sabire KARAÇALI Y1 - 2016 PY - 2016 N1 - doi: 10.17776/csj.10236 DO - 10.17776/csj.10236 T2 - Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi JF - Journal JO - JOR SP - 109 EP - 119 VL - 37 IS - 2 SN - 1300-1949-1300-1949 M3 - doi: 10.17776/csj.10236 UR - https://doi.org/10.17776/csj.10236 Y2 - 2020 ER -
EndNote %0 Cumhuriyet Üniversitesi Fen-Edebiyat Fakültesi Fen Bilimleri Dergisi Expression of Extracellular Matrix Proteins in Basal Membranes During Fetal Nephron Development in Mice %A Beyhan GÜRCÜ , İbrahim TUĞLU , Sabire KARAÇALI %T Expression of Extracellular Matrix Proteins in Basal Membranes During Fetal Nephron Development in Mice %D 2016 %J Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi %P 1300-1949-1300-1949 %V 37 %N 2 %R doi: 10.17776/csj.10236 %U 10.17776/csj.10236
ISNAD GÜRCÜ, Beyhan , TUĞLU, İbrahim , KARAÇALI, Sabire . "Expression of Extracellular Matrix Proteins in Basal Membranes During Fetal Nephron Development in Mice". Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi 37 / 2 (April 2016): 109-119 . https://doi.org/10.17776/csj.10236
AMA GÜRCÜ B , TUĞLU İ , KARAÇALI S . Expression of Extracellular Matrix Proteins in Basal Membranes During Fetal Nephron Development in Mice. Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi. 2016; 37(2): 109-119.
Vancouver GÜRCÜ B , TUĞLU İ , KARAÇALI S . Expression of Extracellular Matrix Proteins in Basal Membranes During Fetal Nephron Development in Mice. Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi. 2016; 37(2): 119-109.