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The Protective Effect of Green Tea Extract on Formaldehyde Toxicity: Genotoxic Evaluation in Swiss Albino Mice.

Year 2015, Volume: 36 Issue: 2, 63 - 73, 21.01.2015

Betül Taşlı Figen Çiçek Emine Yalçın , Güray Demirtaş Kültiğin Çavuşoğlu

Abstract

In the present study, protective effect of green tea extract against genotoxic effects of formaldehyde on Swiss albino mice were investigated. For this aim, we used the frequency of micronucleus, chromosomal aberrations, abnormal metaphase number and mitotic index as indicators of toxicity. Animals were randomly divided into six groups of six animals each. Green tea extract was applied with two different doses (50 and 150 mg/kg) and  formaldehyde was treated with the dosage of 200 mg/kg. All mice were sacrificed at the end of treatment period and the related tissues were isolated.

Formaldehyde alone-treated mice presented higher frequencies micronucleus, chrosomal aberration and abnormal metaphases compared with the controls; moreover, the mitotic index was lower than in controls. Oral treatment with gren tea extract significantly ameliorated the indices of genotoxicity induced by formaldehyde. Both doses of Green Tea extract tested provided significant protection against formaldehyde-induced toxicity, and its strongest effect was observed at the dose level of 150 mg/kg of body weight. The results obtained from this study suggest that Green Tea is a effective protective agent against Formaldehyde induced toxicity.

Keywords

Formaldehyde Green Tea extract Chromosomal aberration Micronucleus Mitotic index

References

  • Borzov I.N., Beta-decay rates, Nucl.Phys.A 777, 645-675 (2006).
  • B. Zuckerman, D. Buhl, P. Palmer, L. E. Snyder, Observation of interstellar formaldehyde. Astrophys. J. 1970, 160: 485–506. www.greenpackonline.org.
  • R.W. Wood, J.B. Coleman, Behavioral evaluation of the irritant properties of formaldehyde. Toxicol Appi Pharmacol. 1995, 30: 67-72.
  • J.B. Morris, Dosimetry, toxicity and carcinogenicity of inspired asetaldehyde in rat. Mutat Res. 1997, 380: 113-124.
  • B.A. Sorg, J.R. Willis, T.C. Nowatka ,C. Ulibarri, R.E. See, H.H. Westberg, Proposal animal neurosensitization model for multiple chemical sensitivity in studies with formalin. Toxicology. 1996, 11: 135-145.
  • H. Heck, M. Casanova, T.B. Starr, Formaldehyde toxicity: New understanding. Crit. Rev. Toxicol. 1990, 397–426.
  • R.C. Grafstrom, R.D. Curren, L.L. Yang, C.C. Harris, Genotoxicity of formaldehyde in cultured human bronchial fibroblast. Science. 1985, 228: 89-91.
  • E.N. Schachter, T.J. Witek, T. Tosun, B.P. Leaderer, G.J. Beck, A study of respiratory effects from exposure to 2 ppm formaldehyde in healthy subjects. Arch Environ Health. 1986, 41: 229-239.
  • D.O. Adams, T.A. Hamilton, L.D. Laurer, J.H. Dean, The effects of formaldehyde exposure upon the mononuclear phagocyte system of mice. Toxicol Appi Pharmacol. 1987, 88: 165–174.
  • D.J. Green, L.R. Sauder, T.J. Kulle, R. Bascom, Acute response to 3.0 ppm formaldehyde in exercising healthy nonsmokers and asthmatics. Am. Rev. Respir Dis. 1987, 135: 1261–66.
  • A.E. Smith, Formaldehyde. Occup Med. 1992, 42, 83-88.
  • J. Shaham, Y. Bomstein, A. Meltzer, Z. Kaufman, E. Palma, J. Ribak, DNA-protein crosslinks, a biomarker of exposure to formaldehyde in vitro and in vivo studies. Carcinogenesis 1996, 17:121-125.
  • A.A. Ensafi, S. Abassi, Sensitive reaction rate method for the determination on low lewels of formaldehyde with photometric detection. Fresenius J. Anal Chem. 1999, 363:376-379.
  • S.E. Usanmaz, E.S. Akarsu, N. Vural, Neurotoxic effects of acute and subacute formaldehyde exposures in mice. Envir Toxicol Pharmacol. 2002, 11, 93
  • R.C. Grafstrom, J. Albert, J.R. Fornace, H. Autrup, J.F. Lechner, C.C. Harris, Formaldehyde damage to DNA and inhibition of DNA repair in human bronchial cells. Science 1983,216-218.
  • M. Casanova, H.A.D. Heck, J.I. Everitt, W.W. Harrington, J.A. Popp, Formaldehyde concentrations in the blood of rhesus monkeys after inhalation exposure. Food Chem. Toxicol 1988, 220:216-218.
  • J.K. McLaughlin, Formaldehyde and cancer: A critical review. Int. Arch. Occup Environ Health 1994, 66:295-301.
  • V.S. Goldmacher, W.G. Thilly, Formaldehyde is mutagenic for cultured human cells Mutat. Res. 1983,116:417-22.
  • A.T. Natarajan, F. Darroudi, C.J.M. Bussman, A.C. van Kesteren-van Leeuven, Evaluation of the mutagenecity of formaldehyde in mammalian cytogenetic assays in vivo and in vitro. Mutat. Res. 1983,122:355-60.
  • N. Sharma, P. Trikha, M. Athar, S. Raisuddin, Protective effect of Cassza Occzdentalis extract on chemical-induced chromosomal aberrations in mice. Drug. Chem. Toxicol. 1999, 22 (4): 643–653.
  • N. İlhan, D. Seçkin, Protective effect of Nigella sativa seeds on CCI4-induced Hepatotoxicity. Fırat Üniversitesi Sağlık Bilimleri Tıp Dergisi. 2005, 19 (3): 175–179.
  • A.A. Hamza, A. Amin, Apium graveolens modulates sodium valproate-induced reproductive toxicity in rats. J. Exp. Zool. A Ecol. Genet. Physiol.2007, 307A (4): 199–206.
  • F. Nanjo, M. Mori, K. Goto, Y. Hara,Radical scavenging activity of tea catechins and their related compounds. Biosci Biotechnol Biochem.1999, 63: 1621–1623.
  • M.W.L. Koo, C.H. Cho,Pharmacological effects of green tea on the gastrointestinal system. European Journal of Pharmacology. 2004, 500:177– 185.
  • S. Tas, E. Sarandol, S. Ziyanok, K. Aslan, M. Dirican,Effects of green tea on serum paraoxonase/arylesterase activities. Nutrition Research. 2005, 25:1061- 10
  • M.A. Te-Hsiu, X. Zhou, G.F. Loarco, G.G. Arreola, S.U. Lecona, Mouse–erythrocyte micronucleus (MUS-EMN) assay on the clastogenicity of industrial wastewater. Rev. Int. Contam Ambient. 1995, 11: 95–98.
  • N. Titenko-Holland, G. Windham, P. Kolachana, F. Reinisch, S. Parvatham, A.M. Osorio, M. T. Smith, Genotoxicity of malathion in human lymphocytes assessed using the micronucleus assay in vitro and in vivo: a study of malathion-exposed workers. Mutat. Res. 1997, 388: 85–95.
  • Y. Özkul, H. Dönmez, A. Erenmemişoğlu, H. Demirtas, N. İmamoğlu, Induction of micronuclei by smokeless tobacco on buccal mucosa cells of habitual users. Mutagenesis. 1997, 12: 285–287.
  • K. Çavuşoğlu, K. Yapar, E. Yalçın, The Royal Jelly (Honey Bee) has a potential antioxidant against cadmium-induced genotoxicity and oxidative stress in Albino mice. J. Med. Food. 2009, 12 (6): 1286–1292.
  • M. Fenench, W.P. Chang, M. Kirsch-Volders, N. Holland, S. Bonassi, E. Zeiger, Human micronucleus project. HUMN project: detailed description of the scoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures. Mutat. Res. 2003, 534: 65–75.
  • D. Beyersmann, S. Hechtenberg, Cadmium, gene regulation, and cellular signaling in mammalian cells. Toxicol Appl. Pharmacol. 1997, 144: 247–261.
  • J.R. Savage, Classification and relationships of induced chromosomal structural changes. J. Med. Genet. 1976, 13: 103–122.
  • H. Mozdarani, S. Kamali, Antigenotoxic effects of cimetidine against benzene induced micronuclei in mouse bone marrow erythrocytes. Toxicol Lett. 1998, 99: 53–
  • A. Celik, B. Mazmanci, Y. Camlica, A. Askin, Cytogenetic effects of lambda- cyalothrin on Wistar rats bone marrow. Mutat. Res. 2003, 539: 91–97.
  • N.U. Karabay, M.G. Oguz, Cytogenetic and genotoxic effects of the insecticides, imidacloprid and methamidophos. Genet. Mol. Res. 2005, 4: 653–662.
  • X. Cao, Z. Cao, X. Zheng, M. Wang, Z. Li, C. Li, J. Wang, D. Long, W. Rang, Micronucleus rate and chromosome aberration of mouse embryo liver induced by formaldehyde. Wei Sheng Yan Jiu. 2009, 38(6): 667-671.
  • S. Coimbra, E. Castro, P. Rocha-Pereira, I. Rebelo, S. Rocha, A. Santos-Silva, The effect of green tea in oxidative stress. Clin. Nutr. 2006, 25: 790–796.
  • P.V.A Babu, K.E. Sabitha, C.S. Shyamaladevi, Green tea extract impedes dyslipidaemia and development of cardiac dysfunction in streptozotocin- diabetic rats. Clin. Exp. Pharmacol Physiol. 2005, 33: 1184–1189.
  • Y.C. Liang, S.Y. LinShiau, C.F. Chen, J.K. Lin,Different tumor-promoting agents are known to induce free radicals in cells. J. Cell. Biochem. 1999, 75:1-12.
  • H. Fujiki, M. Suganuma, S. Okabe, E. Sueoka, K. Suga, K. Imai, K. Nakachi, S. Kimura, Mechanistic findings of green tea as cancer preventive for humans. Proc. Soc. Exp. Biol. Med. 1999, 220:225–228..

Formaldehit Toksisitesine Karşı Yeşil Çay Özütünün Koruyucu Etkisi: Swiss Albino Farelerde Genotoksik Değerlendirme

Year 2015, Volume: 36 Issue: 2, 63 - 73, 21.01.2015

Betül Taşlı Figen Çiçek Emine Yalçın , Güray Demirtaş Kültiğin Çavuşoğlu

Abstract

-

Keywords

Formaldehit Yeşil çay özütü Kromozomal anormallik Mikronukleus Mitotik indeks

References

  • Borzov I.N., Beta-decay rates, Nucl.Phys.A 777, 645-675 (2006).
  • B. Zuckerman, D. Buhl, P. Palmer, L. E. Snyder, Observation of interstellar formaldehyde. Astrophys. J. 1970, 160: 485–506. www.greenpackonline.org.
  • R.W. Wood, J.B. Coleman, Behavioral evaluation of the irritant properties of formaldehyde. Toxicol Appi Pharmacol. 1995, 30: 67-72.
  • J.B. Morris, Dosimetry, toxicity and carcinogenicity of inspired asetaldehyde in rat. Mutat Res. 1997, 380: 113-124.
  • B.A. Sorg, J.R. Willis, T.C. Nowatka ,C. Ulibarri, R.E. See, H.H. Westberg, Proposal animal neurosensitization model for multiple chemical sensitivity in studies with formalin. Toxicology. 1996, 11: 135-145.
  • H. Heck, M. Casanova, T.B. Starr, Formaldehyde toxicity: New understanding. Crit. Rev. Toxicol. 1990, 397–426.
  • R.C. Grafstrom, R.D. Curren, L.L. Yang, C.C. Harris, Genotoxicity of formaldehyde in cultured human bronchial fibroblast. Science. 1985, 228: 89-91.
  • E.N. Schachter, T.J. Witek, T. Tosun, B.P. Leaderer, G.J. Beck, A study of respiratory effects from exposure to 2 ppm formaldehyde in healthy subjects. Arch Environ Health. 1986, 41: 229-239.
  • D.O. Adams, T.A. Hamilton, L.D. Laurer, J.H. Dean, The effects of formaldehyde exposure upon the mononuclear phagocyte system of mice. Toxicol Appi Pharmacol. 1987, 88: 165–174.
  • D.J. Green, L.R. Sauder, T.J. Kulle, R. Bascom, Acute response to 3.0 ppm formaldehyde in exercising healthy nonsmokers and asthmatics. Am. Rev. Respir Dis. 1987, 135: 1261–66.
  • A.E. Smith, Formaldehyde. Occup Med. 1992, 42, 83-88.
  • J. Shaham, Y. Bomstein, A. Meltzer, Z. Kaufman, E. Palma, J. Ribak, DNA-protein crosslinks, a biomarker of exposure to formaldehyde in vitro and in vivo studies. Carcinogenesis 1996, 17:121-125.
  • A.A. Ensafi, S. Abassi, Sensitive reaction rate method for the determination on low lewels of formaldehyde with photometric detection. Fresenius J. Anal Chem. 1999, 363:376-379.
  • S.E. Usanmaz, E.S. Akarsu, N. Vural, Neurotoxic effects of acute and subacute formaldehyde exposures in mice. Envir Toxicol Pharmacol. 2002, 11, 93
  • R.C. Grafstrom, J. Albert, J.R. Fornace, H. Autrup, J.F. Lechner, C.C. Harris, Formaldehyde damage to DNA and inhibition of DNA repair in human bronchial cells. Science 1983,216-218.
  • M. Casanova, H.A.D. Heck, J.I. Everitt, W.W. Harrington, J.A. Popp, Formaldehyde concentrations in the blood of rhesus monkeys after inhalation exposure. Food Chem. Toxicol 1988, 220:216-218.
  • J.K. McLaughlin, Formaldehyde and cancer: A critical review. Int. Arch. Occup Environ Health 1994, 66:295-301.
  • V.S. Goldmacher, W.G. Thilly, Formaldehyde is mutagenic for cultured human cells Mutat. Res. 1983,116:417-22.
  • A.T. Natarajan, F. Darroudi, C.J.M. Bussman, A.C. van Kesteren-van Leeuven, Evaluation of the mutagenecity of formaldehyde in mammalian cytogenetic assays in vivo and in vitro. Mutat. Res. 1983,122:355-60.
  • N. Sharma, P. Trikha, M. Athar, S. Raisuddin, Protective effect of Cassza Occzdentalis extract on chemical-induced chromosomal aberrations in mice. Drug. Chem. Toxicol. 1999, 22 (4): 643–653.
  • N. İlhan, D. Seçkin, Protective effect of Nigella sativa seeds on CCI4-induced Hepatotoxicity. Fırat Üniversitesi Sağlık Bilimleri Tıp Dergisi. 2005, 19 (3): 175–179.
  • A.A. Hamza, A. Amin, Apium graveolens modulates sodium valproate-induced reproductive toxicity in rats. J. Exp. Zool. A Ecol. Genet. Physiol.2007, 307A (4): 199–206.
  • F. Nanjo, M. Mori, K. Goto, Y. Hara,Radical scavenging activity of tea catechins and their related compounds. Biosci Biotechnol Biochem.1999, 63: 1621–1623.
  • M.W.L. Koo, C.H. Cho,Pharmacological effects of green tea on the gastrointestinal system. European Journal of Pharmacology. 2004, 500:177– 185.
  • S. Tas, E. Sarandol, S. Ziyanok, K. Aslan, M. Dirican,Effects of green tea on serum paraoxonase/arylesterase activities. Nutrition Research. 2005, 25:1061- 10
  • M.A. Te-Hsiu, X. Zhou, G.F. Loarco, G.G. Arreola, S.U. Lecona, Mouse–erythrocyte micronucleus (MUS-EMN) assay on the clastogenicity of industrial wastewater. Rev. Int. Contam Ambient. 1995, 11: 95–98.
  • N. Titenko-Holland, G. Windham, P. Kolachana, F. Reinisch, S. Parvatham, A.M. Osorio, M. T. Smith, Genotoxicity of malathion in human lymphocytes assessed using the micronucleus assay in vitro and in vivo: a study of malathion-exposed workers. Mutat. Res. 1997, 388: 85–95.
  • Y. Özkul, H. Dönmez, A. Erenmemişoğlu, H. Demirtas, N. İmamoğlu, Induction of micronuclei by smokeless tobacco on buccal mucosa cells of habitual users. Mutagenesis. 1997, 12: 285–287.
  • K. Çavuşoğlu, K. Yapar, E. Yalçın, The Royal Jelly (Honey Bee) has a potential antioxidant against cadmium-induced genotoxicity and oxidative stress in Albino mice. J. Med. Food. 2009, 12 (6): 1286–1292.
  • M. Fenench, W.P. Chang, M. Kirsch-Volders, N. Holland, S. Bonassi, E. Zeiger, Human micronucleus project. HUMN project: detailed description of the scoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures. Mutat. Res. 2003, 534: 65–75.
  • D. Beyersmann, S. Hechtenberg, Cadmium, gene regulation, and cellular signaling in mammalian cells. Toxicol Appl. Pharmacol. 1997, 144: 247–261.
  • J.R. Savage, Classification and relationships of induced chromosomal structural changes. J. Med. Genet. 1976, 13: 103–122.
  • H. Mozdarani, S. Kamali, Antigenotoxic effects of cimetidine against benzene induced micronuclei in mouse bone marrow erythrocytes. Toxicol Lett. 1998, 99: 53–
  • A. Celik, B. Mazmanci, Y. Camlica, A. Askin, Cytogenetic effects of lambda- cyalothrin on Wistar rats bone marrow. Mutat. Res. 2003, 539: 91–97.
  • N.U. Karabay, M.G. Oguz, Cytogenetic and genotoxic effects of the insecticides, imidacloprid and methamidophos. Genet. Mol. Res. 2005, 4: 653–662.
  • X. Cao, Z. Cao, X. Zheng, M. Wang, Z. Li, C. Li, J. Wang, D. Long, W. Rang, Micronucleus rate and chromosome aberration of mouse embryo liver induced by formaldehyde. Wei Sheng Yan Jiu. 2009, 38(6): 667-671.
  • S. Coimbra, E. Castro, P. Rocha-Pereira, I. Rebelo, S. Rocha, A. Santos-Silva, The effect of green tea in oxidative stress. Clin. Nutr. 2006, 25: 790–796.
  • P.V.A Babu, K.E. Sabitha, C.S. Shyamaladevi, Green tea extract impedes dyslipidaemia and development of cardiac dysfunction in streptozotocin- diabetic rats. Clin. Exp. Pharmacol Physiol. 2005, 33: 1184–1189.
  • Y.C. Liang, S.Y. LinShiau, C.F. Chen, J.K. Lin,Different tumor-promoting agents are known to induce free radicals in cells. J. Cell. Biochem. 1999, 75:1-12.
  • H. Fujiki, M. Suganuma, S. Okabe, E. Sueoka, K. Suga, K. Imai, K. Nakachi, S. Kimura, Mechanistic findings of green tea as cancer preventive for humans. Proc. Soc. Exp. Biol. Med. 1999, 220:225–228..
There are 40 citations in total.

Details

Primary Language Turkish
Journal Section Editorial
Authors

Betül Taşlı This is me

Figen Çiçek This is me

Emine Yalçın

Güray Demirtaş This is me

Kültiğin Çavuşoğlu This is me

Publication Date January 21, 2015
Published in Issue Year 2015 Volume: 36 Issue: 2

Cite

APA Taşlı, B., Çiçek, F., Yalçın, E., Demirtaş, G., et al. (2015). Formaldehit Toksisitesine Karşı Yeşil Çay Özütünün Koruyucu Etkisi: Swiss Albino Farelerde Genotoksik Değerlendirme. Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi, 36(2), 63-73.
AMA Taşlı B, Çiçek F, Yalçın E, Demirtaş G, Çavuşoğlu K. Formaldehit Toksisitesine Karşı Yeşil Çay Özütünün Koruyucu Etkisi: Swiss Albino Farelerde Genotoksik Değerlendirme. Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi. March 2015;36(2):63-73.
Chicago Taşlı, Betül, Figen Çiçek, Emine Yalçın, Güray Demirtaş, and Kültiğin Çavuşoğlu. “Formaldehit Toksisitesine Karşı Yeşil Çay Özütünün Koruyucu Etkisi: Swiss Albino Farelerde Genotoksik Değerlendirme”. Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi 36, no. 2 (March 2015): 63-73.
EndNote Taşlı B, Çiçek F, Yalçın E, Demirtaş G, Çavuşoğlu K (March 1, 2015) Formaldehit Toksisitesine Karşı Yeşil Çay Özütünün Koruyucu Etkisi: Swiss Albino Farelerde Genotoksik Değerlendirme. Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi 36 2 63–73.
IEEE B. Taşlı, F. Çiçek, E. Yalçın, G. Demirtaş, and K. Çavuşoğlu, “Formaldehit Toksisitesine Karşı Yeşil Çay Özütünün Koruyucu Etkisi: Swiss Albino Farelerde Genotoksik Değerlendirme”, Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi, vol. 36, no. 2, pp. 63–73, 2015.
ISNAD Taşlı, Betül et al. “Formaldehit Toksisitesine Karşı Yeşil Çay Özütünün Koruyucu Etkisi: Swiss Albino Farelerde Genotoksik Değerlendirme”. Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi 36/2 (March 2015), 63-73.
JAMA Taşlı B, Çiçek F, Yalçın E, Demirtaş G, Çavuşoğlu K. Formaldehit Toksisitesine Karşı Yeşil Çay Özütünün Koruyucu Etkisi: Swiss Albino Farelerde Genotoksik Değerlendirme. Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi. 2015;36:63–73.
MLA Taşlı, Betül et al. “Formaldehit Toksisitesine Karşı Yeşil Çay Özütünün Koruyucu Etkisi: Swiss Albino Farelerde Genotoksik Değerlendirme”. Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi, vol. 36, no. 2, 2015, pp. 63-73.
Vancouver Taşlı B, Çiçek F, Yalçın E, Demirtaş G, Çavuşoğlu K. Formaldehit Toksisitesine Karşı Yeşil Çay Özütünün Koruyucu Etkisi: Swiss Albino Farelerde Genotoksik Değerlendirme. Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi. 2015;36(2):63-7.