Investigating the Effect of Heat Shock on Chromosomal Aberration of Vicia dasycarpa

Year 2015, Volume: 36 Issue: 3, 657 - 663, 13.05.2015

Sanaz Fareghi , Varahram Rashidi Ahmad Razban Haghighi

Abstract

Abstract. One of the wide applications of cytogenetic is evaluation of environmental factors on chromosomes. In this study, some seeds were boiled in bath water of 900 C for 3 minutes in order to study effects of heat shock on chromosomal aberration of hairy vetch. Then root-tip meristems of both kinds of seeds (i.e. normal seeds and seeds obtained under heat shock conditions) were studied from metaphase mitosis aspect. Normally plant is diploid with basic chromosome number x=7, (2n=2x=14). Cytogenetic studies of the seeds under heat shock conditions indicated mixoploidy state, in a way that diploid, aneuploid and tetraploid cells were observed in a root. Results of variance analysis and comparison of average normal treatments with heat shock treatments in diploid state indicated increased length of chromosomes and increased amount of genome. No aberration of chromosomal size was observed in tetraploid cells compared to normal state, just number of chromosomes increased twofold (2n=4X=28). Moreover study of some morphological traits indicated significant difference in the plant height of heat shock conditions compared to normal plants.

Keywords

meristem, mixoploidy, aneuploid, metaphase, genome

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