Cytotoxic effect of Zataria multiflora on breast cancer cell line (MCF-7) and normal fibroblast cells

Year 2015, Volume: 36 Issue: 3, 1895 - 1904, 13.05.2015

Farkhondeh Nemati , Maryam Janitermi

Abstract

Abstract. The aim of this study was to evaluate the cytotoxic effects of Zataria multifloraessential oilon the human breast cancer cell line (MCF-7) and fibroblast cells. The essential oil of Z. multiflorawas obtained by distillation. Cells were cultured with various concentrations of Z. multiflora essential oil(6.25, 12.5, 25, 50, 100, 200 and 400 µg/ml) for 24, 48 and 72 hours by MTT testAfter 24, 48 and 72 h results revealed that the more effect of Z. multifloraessenceon the growth inhibitory of MCF-7 cell line was observed at 200 μg/ml (102.01, 105.17 and 99.96% respectively). These effects became more prominent after 48 hours. The IC50 values after 24, 48 and 72 h were 158.97, 30.44 and 36.63 mg/ml respectively. Also results showed that more effect of Z. multifloraessenceon the growth inhibitory of normal fibroblast cell was observed at 100 μg/ml after 48h (98.82%).

Keywords

Zataria multiflora, cytotoxic effect, MCF-7 cells, fibroblast cells, MTT assay

References

• Coleman, W.B. and Tsongalis, G.J. (2002), “The Molecular Basis of Human Cancer,” Human Press Inc., Totowa, N J, pp. 347-363.
• McPherson, K., Steel, C.M. and Dixon, J.M. (2000), “Breast cancer-epidemiology, risk factors, and genetics,” BMJ, 321(9), pp. 624-628.
• Valeriote, F., Grieshaber, C.K., Media, J., Pietraszkewics, H., Hoffmann, J., Pan, M. and McLaughlin, S. (2002) “Discovery and development of anticancer agents from plants,” Journal of Experimental Therapeutics and Oncology, 2, pp. 228-236.
• Kinghorn, A.D., Farnsworth, N.R., Soejarto, D.D., Cordell, G.A., Swanson, S.M., Pezzuto, J.M., Wani, M.C., Wall, M.E., Oberlies, N.H., David, J., Keoll, D.J., Kramer, R.A., Rose, W.C., Vite, G.D., Fairchild, C.R., Peterson, R.W. and Wild, R. (2003), “Novel Strategies for the Discovery of Plant-Derived Anticancer Agents,” Pharmaceutical Biology, 41, pp. 53-67.
• Rezaie-Tavirani, M., Fayazfar, S., Heydari-Keshel, S., Rezaee, M.B., Zamanian-Azodi, M., Rezaei-Tavirani, M. and Khodarahmi, R. 2013. (2006), “Effect of essential oil of Rosa Damascena on human colon cancer cell line SW742,” Gastroenterol Hepatol Bed Bench , 6(1), pp.25-31.
• Chin, Y.W., Balunas, M.J., Chai, H.B. and Kinghorn, A.D. (2010), “Drug Discovery from Natural Sources,” The AAPS Journal, 8(2), pp. 239-253.
• Azizi, M.H. (2008), “History of Ancient Medicine in Iran,” Arch Iranian Med, 11, pp.116 – 119.
• Ali, M.S., Saleem, M., Ali, Z. and Ahmad, V.U. (2000), “Chemistry of Zataria multiflora (Lamiaceae),” Phytochem, 55, pp.933–6.
• Hosseinzadeh, H., Ramezani, M. and Salmani, G. (2000), “Antinociceptive, anti- inflammatory and acute toxicity effects of Zataria multiflora Boiss. extracts in mice and rats,” J Ethnopharmacol, 73, pp. 379–85.
• Jafarian-Dehkordi, A., Emami, S.A., Saeidi, M. and Sadeghi, H. (2004), “Cytotoxicologic studies of the extracts of iranian Juniperus sabina and Platycladus orientalis on cancer cells,” J Res Med Sci, 5, pp.205–9.
• Saeedi-Saravi, S.S. and Shokrzadeh, M. (2008), “The study of hepatic and renal disorders in mice which were administered ethyl acetate extract of plant Sambucus ebulus Intraperituenally (IP) and effect of vitamins C and E on prevention of its disorders,” Toxicol Lett,180, pp.57–58.
• Shaffiee, A. and Javidnia, K. (1997), “Composition of essential oil of Zataria multiflora,” Planta Med, 63, pp. 371–2.
• Phelan, M.C. (1998), “Basic Techniques for Mammalian to Cell Tissue Culture,” Current protocols in cell biology; 7, pp. 1-10.
• Sylvester, P.W. (2011), “Optimization of the tetrazolium dye (MTT) colorimetric assay for cellular growth and viability,” Methods Mol Biol, 716, pp.157-68.
• Maioli, E., Torricelli, C., Fortino, V., Carlucci, F., Tommassini, V. and Pacini, A. (2009), “Critical appraisal of the MTT assay in the presence of rottlerin and uncouplers,” Biol Proced Online, 3(11), pp. 227-40.
• Zamanian-Azodi, M., Rezaie-Tavirani, M., Heydari- Kashal, S., kalantari, S., Dalilan, S. and Zali, H. (2012), “Proteomics analysis of MKN45 cell line before and after treatment with Lavender aqueous extract,” Gastroenterol Hepatol Bed Bench, 5, pp.35-42.
• Ma, Y., Peng, J., Liu, W., Zhang, P., Huang, L., Gao, B., et al. (2009), “Proteomics identification of desmin as a potential oncofetal diagnostic and prognostic biomarker in colorectal cancer,” Mol Cell Proteomics, pp. 1878-1890.
• Craig, W.J. (1999), “Health-promoting properties of common herbs. Am J Clin Nutr, 70, pp. 491-99.
• Soheili Kashani, M., Rezaei Tavirani, M., Talaei A., and Salami, M. (2011), “Aqueous extract of lavender (Lavandulaangustifolia) improves the spatial performance of a rat model of Alzheimer's disease,” Neurosci Bull, 27, pp. 99-106.
• Zali, H., Rezaei-Tavirani, M. and Azodi, M. (2011), “Gastric cancer: prevention, risk factors and treatment,” Gastroenterol Hepatol Bed Bench, 4, pp. 175-85.
• Lai, P.K. and Roy, J. (2004), “Antimicrobial and chemopreventive properties of herbs and spices,” Curr Med Chem,11, pp.1451–60.
• Alizadeh, A. and Shaabani, M. (2014), “Essential oil composition, total phenolic content and antioxidant activities of Iranian Zataria multiflora Boiss,” International Journal of Biosciences. 4 (4), pp. 97-104.
• Shaffiee, A. and Javidnia, K. (1997), “Composition of essential oil of Zataria multiflora,” Planta Med. 63, pp. 371-372.
• Ebrahimzadeh, H., Yamini, Y., Sefidkon, F., Chaloosi, M. and Pourmortazavi, S.M. (2003), “Chemical composition of the essential oil and supercritical CO2 extracts of Zataria multiflora Boiss,” Food Chem., 83, pp. 357-361.
• Sharififar, F., Moshafi, M.H., Mansouri, S.H., Khodashenas, M. and Khoshnoodi, M. (2007), “In vitro evaluation of antibacterial and antioxidant activities of the essential oil and methanol extract of endemic Zataria multiflora Boiss,” Food Control, 18, pp. 800-805.
• Alipoureskandani, M. (2011), “Effect of Zataria multifloraBoiss essential oil on the growth of bacillus cereus in a commercial chicken soup,” Book of proceedings 6, pp. 389-392.
• Yang, H., Dou, Q.P. (2010), “Targeting apoptosis pathway with natural terpenoids: implications for treatment of breast and prostate cancer,” Curr. Drug Targets, 11(6):, pp.733-744.
• Lee, D.H., Iwanski, G.B. and Thoennissen, N.H. (2010), “Cucurbitacin: ancient compound shedding new light on cancer treatment,” Sci. World J., 10, pp. 413-418.
• Martin, K., Trouche, D., Hagemeier, C., Sİrensen, T.S., Thangue, N.B. and Kouzarides, T. (1995), “Stimulation of E2F1/DP1 transcriptional activity by MDM2 oncoprotein,” Nature, 375, pp. 691-694.
• Xiao, Z.X., Chen, J., Levine, A.J., Modjtahedi, N., Xing, J., Sellers, W.R. and Livingston, D.M. (1995), “Interaction between the retinoblastoma protein and the oncoprotein MDM2,” Nature, 375, pp. 694-698.
• Vaziri-Gohar, A. and Mohammadi, A. (2010), “Epigenetic effects of carcinogens,” J. Biol. Sci., 10(3), pp. 200-208.
• He, L., Mo, H., Hadisusilo, S., Qureshi, A.A. and Elson, C.E. (1997), “Isoprenoids suppress the growth of murine B16 melanomas in vitro and in vivo,” J Nutr 127, pp. 668– 674.
• Stammati, A., Bonsi, P., Zucco, F., Moezelaar, R., Alakomi, H.L. and Von-Wright, A. (1999), “Toxicity of selected plant volatiles in microbial and mammalian short-term assays,” Food Chem Toxicol 37, pp. 813–823.
• Zeytinoglu, M., Aydin, S., Ztrk, Y., Hsn, K. and Baser, C. (1998), “Inhibitory effects of carvacrol on DMBA induced pulmonary tumorigenesis in rats,” Acta Pharmaceutica Turcica 40, pp. 93–98.
• Zeytinoglu, H., Incesu, Z. and Baser, K. (2003), “Inhibition of DNA synthesis by Carvacrol in mouse myoblast cells bearing a human N-RAS oncogene,” Phytomedicine 10, pp. 292– 299.
• Koparal, A.T. and Zeytinoglu, M. (2003), “Effects of carvacrol on a human non-small cell lung cancer (NSCLC) cell line, A549,” Cytotechnology 43, pp. 149–154.
• Ipek, E., Tuylu, B.A. and Zeytinoglu, H. (2003), “Effects of carvacrol on sister chromatid exchanges in human lymphocyte cultures,” Cytotechnology 43, pp. 145–148.
• Arunasree, K. (2010), “Anti-proliferative effects of carvacrol on a human metastatic breast cancer cell line, MDA-MB 231,” Phytomedicine 17, pp. 581–588.
• Brochhausen, C., Lehmann, M., Zehbe, R., Watzer, B., Grad, S., Meurer, A., et al. (2009), “Tissue engineering of cartilage and bone: growth factors and signaling molecules,” Orthopade, 38, pp. 1053-62.