Response of antioxidant enzyme activity and pigment content in common bean (Phaseolus vulgaris L.) seedlings under salt stress

Year 2015, Volume: 36 Issue: 3, 2828 - 2833, 13.05.2015

Sajjad Moharramnejad , Tofigh Taherkhanı

Abstract

Abstract. In order to evaluate the effects of osmotic stress on fresh weight, pigment content and responses of antioxidant enzymes including superoxide dismutase (SOD), peroxidase (POX) and catalase (CAT), a factorial experiment was carried out under laboratory conditions with five common bean genotypes and two salt stress levels induced by NaCl (control and 400 mM). Electrophoretic analyses were performed for three antioxidant enzymes SOD, POX and CAT in leafs of common bean genotype seedlings using 8% slab polyacrylamide gels. For SOD, POX and CAT, three, three and one isozymes were observed, respectively. Salinity was decreased fresh weight and chlorophyll content. Anthocyanins and carotenoids concentration was increased in common bean genotypes. Statistical analysis showed that salinity stress has a significant effect on fresh weight, pigment content and enzymatic activities in common bean seedlings. SOD, POX and CAT activities were increased significantly in the salt stress compared with control condition about 40, 21 and 30.5 percent, respectively. These results seem to indicate that 41218 genotype of common bean tolerance to salt stress is associated with enhance activity of antioxidant enzymes. 

Keywords

Antioxidant enzyme, Common bean, Pigment content, Salinity

References

• Alscher RG, Erturk N, Heath LS.2002. Role of superoxide dismutases (SODs) in controlling oxidative stress. J Exp Bot 53:1331-1341.
• Ashraf M. 2009. Biotechnological approach of improving plant salt tolerance using antioxidants as markers.BiotechAdv 27: 84–93.
• Asish KP, Anath BD, Prasanna M. 2004. Defense potentials to NaCl in mangrove,
• Bruguieraparviflora: Differential changes of isoforms of some antioxidant enzymes. J Plant Physio 161: 531-542. Comba ME, Benavides MP, Gallego SM, Tomaro ML. 1998. Relationship between nitrogen fixation and oxidative stress induction in nodules of salt-treated soybean plants.EnvExp Bot 60:155-126.
• Gaber MA. 2010. Antioxidative defense under salt stress. Plant Sign Behav 5: 369-374.
• Gupta KJ, StoimenovaM, Kaiser WM. 2005. In higher plants, only root mitochondria, but not leaf mitochondria reduce nitrite to NO. in vitro and in situ. J Exp Bot 56: 2601–2609.
• NageshBabu R, Devaraj VR. 2008. High temperature and salt stress response in French bean
• (Phaseolus vulgaris). Aust J Crop Sci 2: 40-48. Neill SO, Gould KS.2003.Anthocyanins in leaves: light attenuators or antioxidants? Funct Plant Biol 30: 865–873.
• Noctor G, Foyer CH. 1998.Ascorbate and glutathione: keeping active oxygen under control.
• Ann Rev Plant Physiol Plant MolBiol49:249-279. Mittler R,Vanderauwera S,Gollery M, Breusegem F. 2004. Reactive oxygen genenetwork of plants. Trends Plant Sci 9: 490–498.
• Moharramnejad S,Valizadeh M. 2014. Assessment of oxidative stress tolerance in red bean
• (Phaseolus vulgaris L.) seedling under salinity. InterJ Agri Agro Res 5: 49-56. Valizadeh M, Moharamnejad S, Ahmadi M, MohammadzadehJalaly H. 2013. Changes in activity profile of some antioxidant enzymes in alfalfa half-sib families under salt stress. J AgriSciTech 15: 801-809.
• Verma V, Foullces MJ, Worland AJ, Sylvester-Bradley R, Caligari PDS, Snape JW.2004.
• Mapping quantitative trait loci for flag leaf senescence as a yield determinant in winter wheat under optimal and drought-stressed environments. Euphytica, 135: 255–263. Wang W, Kim Y, Lee H, Kim K, Deng X, Kwak S. 2009. Analysis of antioxidant enzyme activity during germination of alfalfa under salt and drought stresses. Plant PhysioBiochem47: 570–577.
• Yaryura P, Cordon G, Leon M, Kerber N, Pucheu N, Rubio N, Garc G, Lagorio G. 2009.