HOST AND PLANT TAXONOMY AS INFLUENTIAL FACTORS IN CONTROLING IN VITRO SPECIFIC CELL-MEDIATED RESPONSE IN HERBIVORES

Vasiu Aurel; Carmen Sandru; Emoke Pall; Emilia Ungureanu; Florina Marian; Silvana Popescu; Alexandra Oprescu; Ciprian Ilasca

doi:10.38093/cupmap.1337829

EN

HOST AND PLANT TAXONOMY AS INFLUENTIAL FACTORS IN CONTROLING IN VITRO SPECIFIC CELL-MEDIATED RESPONSE IN HERBIVORES

Abstract

Objective: This study monitored several alcoholic vegetal extracts from the adjuvant and immune cell stimulating perspective in farmed herbivores, differing by their digestive physiology. Material and Methods: Blood from randomly selected farmed ruminants (Romanian Spotted dairy cows, n=28 and Angora goats, n=19) and Romanian draft horses (n=27) was used to measure the in vitro effects on specific cell-mediated reactivity of alcoholic extracts of Calendula officinalis, Echinacea angustifolia and E. purpurea, by the in vitro whole blood blast transformation test, in 96 well plates, after 60 to 72h of incubation. Cell growth was quantiﬁed by an orto-toluidine technique. Student’s t- test was used to evaluate the statistical significance of the differences. Results&Discussion: The in vitro growth indices were lowest in goats (C. officinalis - 58.52±10.02%, E.angustifolia - 50.06±11.67%, E.purpurea -50.79±10.98%) and higher in bovine (C. officinalis – 69.9±2.65%, E.angustifolia - 74.9±10.1%, p<0.05), and increased towards E. angustifolia versus C. officinalis. In vitro responses to C. officinalis, E. angustifolia and E.purpurea were the most pronounced in horses and similar for all of these extracts. Conclusion: All the extracts showed inhibiting effects in bovine and goats, but not in horses, supporting host- based differences. The biological activity of the tested extracts was plant species dependent.

Keywords

Thanks

The research was supported by current funds of the Discipline of Infectious diseases. The authors wish to thank for laboratory assistance to biotechnologist Eng. Elena Marian and for technical support to senior technician Traian Jucan.

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Details

Primary Language

English

Subjects

Industrial Crops

Journal Section

Research Article

Authors

Vasiu Aurel ^*
0000-0002-3160-8401
Romania

Carmen Sandru
0000-0001-8615-1202
Romania

Emoke Pall
0000-0002-7919-7381
Romania

Emilia Ungureanu
0000-0003-3438-8931
Romania

Florina Marian
0000-0002-6111-7881
Romania

Silvana Popescu
0000-0002-7355-2688
Romania

Alexandra Oprescu
0009-0005-8121-803X
Romania

Ciprian Ilasca
0009-0009-3103-5955
Romania

Early Pub Date

September 21, 2023

Publication Date

December 25, 2023

Submission Date

August 9, 2023

Acceptance Date

September 4, 2023

Published in Issue

Year 2023 Volume: 6 Number: 2

DOI

https://doi.org/10.38093/cupmap.1337829

IZ

https://izlik.org/JA54XU63PL

Cite

RIS / Bibtex

APA

Aurel, V., Sandru, C., Pall, E., Ungureanu, E., Marian, F., Popescu, S., Oprescu, A., & Ilasca, C. (2023). HOST AND PLANT TAXONOMY AS INFLUENTIAL FACTORS IN CONTROLING IN VITRO SPECIFIC CELL-MEDIATED RESPONSE IN HERBIVORES. Current Perspectives on Medicinal and Aromatic Plants, 6(2), 78-83. https://doi.org/10.38093/cupmap.1337829

AMA

1.Aurel V, Sandru C, Pall E, et al. HOST AND PLANT TAXONOMY AS INFLUENTIAL FACTORS IN CONTROLING IN VITRO SPECIFIC CELL-MEDIATED RESPONSE IN HERBIVORES. CUPMAP. 2023;6(2):78-83. doi:10.38093/cupmap.1337829

Chicago

Aurel, Vasiu, Carmen Sandru, Emoke Pall, et al. 2023. “HOST AND PLANT TAXONOMY AS INFLUENTIAL FACTORS IN CONTROLING IN VITRO SPECIFIC CELL-MEDIATED RESPONSE IN HERBIVORES”. Current Perspectives on Medicinal and Aromatic Plants 6 (2): 78-83. https://doi.org/10.38093/cupmap.1337829.

EndNote

Aurel V, Sandru C, Pall E, Ungureanu E, Marian F, Popescu S, Oprescu A, Ilasca C (December 1, 2023) HOST AND PLANT TAXONOMY AS INFLUENTIAL FACTORS IN CONTROLING IN VITRO SPECIFIC CELL-MEDIATED RESPONSE IN HERBIVORES. Current Perspectives on Medicinal and Aromatic Plants 6 2 78–83.

IEEE

[1]V. Aurel et al., “HOST AND PLANT TAXONOMY AS INFLUENTIAL FACTORS IN CONTROLING IN VITRO SPECIFIC CELL-MEDIATED RESPONSE IN HERBIVORES”, CUPMAP, vol. 6, no. 2, pp. 78–83, Dec. 2023, doi: 10.38093/cupmap.1337829.

ISNAD

Aurel, Vasiu - Sandru, Carmen - Pall, Emoke - Ungureanu, Emilia - Marian, Florina - Popescu, Silvana - Oprescu, Alexandra - Ilasca, Ciprian. “HOST AND PLANT TAXONOMY AS INFLUENTIAL FACTORS IN CONTROLING IN VITRO SPECIFIC CELL-MEDIATED RESPONSE IN HERBIVORES”. Current Perspectives on Medicinal and Aromatic Plants 6/2 (December 1, 2023): 78-83. https://doi.org/10.38093/cupmap.1337829.

JAMA

1.Aurel V, Sandru C, Pall E, Ungureanu E, Marian F, Popescu S, Oprescu A, Ilasca C. HOST AND PLANT TAXONOMY AS INFLUENTIAL FACTORS IN CONTROLING IN VITRO SPECIFIC CELL-MEDIATED RESPONSE IN HERBIVORES. CUPMAP. 2023;6:78–83.

MLA

Aurel, Vasiu, et al. “HOST AND PLANT TAXONOMY AS INFLUENTIAL FACTORS IN CONTROLING IN VITRO SPECIFIC CELL-MEDIATED RESPONSE IN HERBIVORES”. Current Perspectives on Medicinal and Aromatic Plants, vol. 6, no. 2, Dec. 2023, pp. 78-83, doi:10.38093/cupmap.1337829.

Vancouver

1.Vasiu Aurel, Carmen Sandru, Emoke Pall, Emilia Ungureanu, Florina Marian, Silvana Popescu, Alexandra Oprescu, Ciprian Ilasca. HOST AND PLANT TAXONOMY AS INFLUENTIAL FACTORS IN CONTROLING IN VITRO SPECIFIC CELL-MEDIATED RESPONSE IN HERBIVORES. CUPMAP. 2023 Dec. 1;6(2):78-83. doi:10.38093/cupmap.1337829