EVALUATION OF GENISTEIN CONTENT IN CHICKPEA (CICER ARIETINUM L.) AND MUNG BEAN VIGNA RADIATA L.) SPROUTS GERMINATED UNDER DIFFERENT CONDITIONS

Abstract

Cicer arietinum L. (chickpea) and Vigna radiata L. (mung bean) are two important pulses, being continuously used by a large number of populations all over the world. Sprouts of both pulses are also gaining importance due to the presence of high level of dietary fibers, vitamin B-Complex, vitamin A, vitamin C, omega-3 fatty acids and proteins. There are lots of studies done on evaluation of total isoflavonoids content present in seeds of various pulses including soybean, chickpea, lentil etc. Various biochemical parameters of sprouts of both pluses have also been studied till date but no specific studies on secondary metabolite content during sprouting have been done. The aim of the present study was an evaluation of growth and genistein content from 1^st day to 6^th day of sprouting, evaluation of different growth conditions (BOD, in vitro, in vivo) for the same parameters along with the effect of  prohexadione on the sprouts of both pluses have been done. Among the three studied genotypes of chickpea, CSCD-884 genotype was the best for the high genistein content. However, in case of mung bean, V. RMW 344 genotype contains higher genistein content. With the change in growth condition, in vitro group give best results in genistein content in mung bean sprouts (28.32 fold higher) while in case of chickpea sprouts BOD group give the best result (12.8 fold higher). Remarkably, lower concentrations of prohexadione-pure treatment enhance the genistein content in both the chickpea and mung bean sprouts at BOD condition. A significant increase was also observed in genistein content in chickpea sprouts while treated with prohexadion-calcium at in vitro condition. This study also reveals that mung beans can supplement higher levels of genistein contents than chickpeas if sprouted at controlled culture conditions. This finding could expand the potential for the development of both pulses sprouts as a functional food by giving stress treatment and enhancing the total isoflavonoid contents. 

Keywords

• Cicer arietinum sprout,Vigna radiata sprout,Elicitation,Prohexadione,Genistein

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