The Effect of Corchorus olitorius L. Extract on Viability of Breast Cancer Cells: A friend or a foe?

Year 2019, , 113 - 118, 26.12.2019

Günsu Soykut Çağsın Eda Becer İhsan Çalış Seda Vatansever

https://doi.org/10.38093/cupmap.652991

Abstract

This Corchorus olitorius L. is
known to be a medicinal plant widely consumed in the Eastern Mediterranean
region and has anti-bacterial, anti-inflammatory and anti-cancer properties.
Breast cancer has one of the highest mortality rates among all cancers.
Therefore, the main aim of the study is to evaluate the effectiveness of C. olitorius plant on viability of
estrogen receptor (ER) positive breast cancer cell line, MCF-7. C. olitorius leaves first extracted with
ethanol, then LC-MS/MS analysis was done for identification of phytochemicals.
MTT assay was used for assessment of cell viability of MCF-7 cells. Cells were
treated with C. olitorius extracts at
five concentrations (5, 10, 20, 50, 100 µg/ml) and four different incubation
periods (24, 48, 72, 96 h). LC-MS/MS analysis identified seven phytochemicals
in the extract, mainly quercetin and caffeoylquinic acid derivatives. MTT
results showed that the extract was only slightly effective in terms of
reduction of cell viability at 50 and 100 µg/ml doses which were incubated for
24 and 48 h. Lower concentration doses did not show any effect in cell
viability of MCF-7 cells. Longer incubation periods tend to increase cell
viability of breast cancer cells. Quercetin identified within the extract  might interfere with ER and promote MCF-7 cell
proliferation. Therefore, in ER positive breast adenocarcinoma, quercetin
intake and doses should carefully be monitored. More studies regarding the
relationship between C. olitorius,
quercetin and breast cancer should be done for further clarification of the
topic.

Keywords

Corchorus olitorius, MCF-7, Breast cancer, Cell viability

Thanks

The authors are thankful to Experimental Health Research Center of Health Sciences and Pharmacognosy laboratories of Near East University for their valuable support during the study.

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