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THE EFFECTS OF THE SIDERITIS OZTURKII EXTRACT ON THE EXPRESSION LEVELS OF SOME APOPTOTIC GENES

Year 2018, Volume: 1 Issue: 1, 8 - 12, 28.07.2018

Abstract

Sideritis öztürkii (Lamiaceae) Aytac & Aksoy is an
endemic species to Turkey and used as herbal tea and folk medicine in Central
Anatolia.  The purpose of this study is
to reveal the apoptotic effects of different extracts derived from S. öztürkii on DLD-1 colorectal cancer
cell line via Real Time PCR technique. By this aim, the expression levels of
apoptotic genes for each extract were determined via Real Time PCR and then the
expression levels were evaluated and compared respectively. Especially, leaf
methanol extract was found to be more effective than leaf aqueous extract in
terms of apoptosis induction. The results infer that the ironwort extracts have
different effects on pro and anti-apoptotic gene expression levels. In
conclusion, the leaf and flower extracts have apoptosis inducing activity in
the different levels on DLD-1 cell line. 

References

  • Aboutabl, E. A., Nassar, M. I., Elsakhawy, F. M., Maklad, Y. A., Osman, A. F., & El-Khrisy, E. A. M. (2002). Phytochemical and pharmacological studies on Sideritis taurica Stephan ex Wild. Journal of ethnopharmacology, 82(2-3), 177-184.
  • Alcaraz MJ, Jimenez MJ, Valverde S, Sanz J, Rabanal RM, Villar A (1989). Anti-inflammatory compounds from Sideritis javalambrensis n-hexane extract. J Nat Prod. 52: 1088–1091.
  • Barberan FAT, Manez S, Villar A (1987). Identification of antiinflamatory agents from Sideritis species growing in Spain. J Nat Prod 50: 313–314.
  • Basile, A., Senatore, F., Gargano, R., Sorbo, S., Del Pezzo, M., Lavitola, A., ... & Vuotto, M. L. (2006). Antibacterial and antioxidant activities in Sideritis italica (Miller) Greuter et Burdet essential oils. Journal of ethnopharmacology, 107(2), 240-248.
  • Charami, M. T., Lazari, D., Karioti, A., Skaltsa, H., Hadjipavlou‐Litina, D., & Souleles, C. (2008). Antioxidant and antiinflammatory activities of Sideritis perfoliata subsp. perfoliata (Lamiaceae). Phytotherapy Research, 22(4), 450-454.
  • Chresta CM, Masters JRW, Hickman JA (1996). Hypersensitivity of human testicular tumors to etoposide-induced apoptosis is associated with p53 and high Bax: Bcl-2 ratio. Cancer Res, 56, 1834-41.
  • Davis P H (1982). Flora of Turkey and East Eagean Island, Vol: 7, Edinburgh University Press, Edinburgh.
  • Demirtas, I., Sahin, A., Ayhan, B., Tekin, S., & Telci, I. (2009). Antiproliferative effects of the methanolic extracts of Sideritis libanotica Labill. subsp. linearis. Records of Natural Products, 3(2), 104.
  • Eliopoulos AG, Kerr DJ, Herod J, et al (1995). The control of apoptosis and drug resistance in ovarian cancer: influence of p53 and Bcl-2. Oncogene, 111217-28.
  • Ezer N, Akcos Y, Rodriguez B, Abbasoğlu U (1995). Sideritis libanotica Labill. subsp. linearis (Bentham) Bornm.’den elde edilen iridoit heteroziti ve antimikrobiyal aktivitesi. Hacettepe Univ J Fac Pharm 15: 15–21.
  • Ezer N, Usluer G, Günes¸ S¸ , Erol K (1994): Antibacterial activity of some Sideritis species. Fitoterapia 65: 549–551.
  • Gelinci, E., Demirelma H., Şimşek Sezer, EN, Uysal, T., Cytotoxic Effects of Sideritis öztürkii Aytaç & Aksoy, XIII. Congress of Ecology and Environment with International Participation, UKECEK 2017, 12-15 September 2017.
  • Guner A, Aslan S, Ekim T, Vural M, Babac MT. 2012. Turkiye Bitkileri Listesi (Damarlı Bitkiler) [A Checklist of the Flora of Turkey (Vascular Plants)]. İstanbul: Nezahat Gokyiğit Botanical Garden and Flora Research Society Publication (in Turkish).
  • Hernández-Pérez, M., & Rabanal, R. M. (2002). Evaluation of the antinflammatory and analgesic activity of Sideritis canariensis var. pannosa in mice. Journal of ethnopharmacology, 81(1), 43-47.
  • Jeremic, I., Tadic, V., Isakovic, A., Trajkovic, V., Markovic, I., Redzic, Z., & Isakovic, A. (2013). The mechanisms of in vitro cytotoxicity of mountain tea, Sideritis scardica, against the C6 glioma cell line. Planta Med, 79(16), 1516-1524.
  • Küpeli, E., Şahin, F. P., Çalış, İ., Yeşilada, E., & Ezer, N. (2007). Phenolic compounds of Sideritis ozturkii and their in vivo anti-inflammatory and antinociceptive activities. Journal of ethnopharmacology, 112(2), 356-360.
  • Oltvai ZN, Milliman CL, Korsmeyer SJ (1993). Bcl-2 heterodimerizes in vivo with a conserved homology, Bax, that accelerates programmed cell death. Cell, 74, 609-19.
  • Sagdic, O., Aksoy, A., Ozkan, G., Ekici, L., & Albayrak, S. (2008). Biological activities of the extracts of two endemic Sideritis species in Turkey. Innovative food science & emerging technologies, 9(1), 80-84.
  • Tadić, V. M., Jeremic, I., Dobric, S., Isakovic, A., Markovic, I., Trajkovic, V., & Arsic, I. (2012). Anti-inflammatory, gastroprotective, and cytotoxic effects of Sideritis scardica extracts. Planta medica, 78(05), 415-427.
  • Tomás-Barberán F., Reijdali M, Harborne J B & Heywood V H (1988). External and vacuolar flavonoids from the Ibero- North African Sideritis species. A chemosystematic approach. Phytochemistry 27: 165- 170.
  • Yumrutas, O., Oztuzcu, S., Pehlivan, M., Ozturk, N., Poyraz, I. E., Iğci, Y. Z., ... & Arslan, A. (2015). Cell viability, anti-proliferation and antioxidant activities of Sideritis syriaca, Tanacetum argenteum subsp. argenteum and Achillea aleppica subsp. zederbaueri on human breast cancer cell line (MCF-7).
  • Zarzuelo A, Garcia E, Jimenez J, Ocete MA, Utrilla P (1993): Anti-inflamatory and anti-ulcerative activity of various species of the genus Sideritis from the Alpujarra region of Spain. Fitoterapia 64: 26–30.
Year 2018, Volume: 1 Issue: 1, 8 - 12, 28.07.2018

Abstract

References

  • Aboutabl, E. A., Nassar, M. I., Elsakhawy, F. M., Maklad, Y. A., Osman, A. F., & El-Khrisy, E. A. M. (2002). Phytochemical and pharmacological studies on Sideritis taurica Stephan ex Wild. Journal of ethnopharmacology, 82(2-3), 177-184.
  • Alcaraz MJ, Jimenez MJ, Valverde S, Sanz J, Rabanal RM, Villar A (1989). Anti-inflammatory compounds from Sideritis javalambrensis n-hexane extract. J Nat Prod. 52: 1088–1091.
  • Barberan FAT, Manez S, Villar A (1987). Identification of antiinflamatory agents from Sideritis species growing in Spain. J Nat Prod 50: 313–314.
  • Basile, A., Senatore, F., Gargano, R., Sorbo, S., Del Pezzo, M., Lavitola, A., ... & Vuotto, M. L. (2006). Antibacterial and antioxidant activities in Sideritis italica (Miller) Greuter et Burdet essential oils. Journal of ethnopharmacology, 107(2), 240-248.
  • Charami, M. T., Lazari, D., Karioti, A., Skaltsa, H., Hadjipavlou‐Litina, D., & Souleles, C. (2008). Antioxidant and antiinflammatory activities of Sideritis perfoliata subsp. perfoliata (Lamiaceae). Phytotherapy Research, 22(4), 450-454.
  • Chresta CM, Masters JRW, Hickman JA (1996). Hypersensitivity of human testicular tumors to etoposide-induced apoptosis is associated with p53 and high Bax: Bcl-2 ratio. Cancer Res, 56, 1834-41.
  • Davis P H (1982). Flora of Turkey and East Eagean Island, Vol: 7, Edinburgh University Press, Edinburgh.
  • Demirtas, I., Sahin, A., Ayhan, B., Tekin, S., & Telci, I. (2009). Antiproliferative effects of the methanolic extracts of Sideritis libanotica Labill. subsp. linearis. Records of Natural Products, 3(2), 104.
  • Eliopoulos AG, Kerr DJ, Herod J, et al (1995). The control of apoptosis and drug resistance in ovarian cancer: influence of p53 and Bcl-2. Oncogene, 111217-28.
  • Ezer N, Akcos Y, Rodriguez B, Abbasoğlu U (1995). Sideritis libanotica Labill. subsp. linearis (Bentham) Bornm.’den elde edilen iridoit heteroziti ve antimikrobiyal aktivitesi. Hacettepe Univ J Fac Pharm 15: 15–21.
  • Ezer N, Usluer G, Günes¸ S¸ , Erol K (1994): Antibacterial activity of some Sideritis species. Fitoterapia 65: 549–551.
  • Gelinci, E., Demirelma H., Şimşek Sezer, EN, Uysal, T., Cytotoxic Effects of Sideritis öztürkii Aytaç & Aksoy, XIII. Congress of Ecology and Environment with International Participation, UKECEK 2017, 12-15 September 2017.
  • Guner A, Aslan S, Ekim T, Vural M, Babac MT. 2012. Turkiye Bitkileri Listesi (Damarlı Bitkiler) [A Checklist of the Flora of Turkey (Vascular Plants)]. İstanbul: Nezahat Gokyiğit Botanical Garden and Flora Research Society Publication (in Turkish).
  • Hernández-Pérez, M., & Rabanal, R. M. (2002). Evaluation of the antinflammatory and analgesic activity of Sideritis canariensis var. pannosa in mice. Journal of ethnopharmacology, 81(1), 43-47.
  • Jeremic, I., Tadic, V., Isakovic, A., Trajkovic, V., Markovic, I., Redzic, Z., & Isakovic, A. (2013). The mechanisms of in vitro cytotoxicity of mountain tea, Sideritis scardica, against the C6 glioma cell line. Planta Med, 79(16), 1516-1524.
  • Küpeli, E., Şahin, F. P., Çalış, İ., Yeşilada, E., & Ezer, N. (2007). Phenolic compounds of Sideritis ozturkii and their in vivo anti-inflammatory and antinociceptive activities. Journal of ethnopharmacology, 112(2), 356-360.
  • Oltvai ZN, Milliman CL, Korsmeyer SJ (1993). Bcl-2 heterodimerizes in vivo with a conserved homology, Bax, that accelerates programmed cell death. Cell, 74, 609-19.
  • Sagdic, O., Aksoy, A., Ozkan, G., Ekici, L., & Albayrak, S. (2008). Biological activities of the extracts of two endemic Sideritis species in Turkey. Innovative food science & emerging technologies, 9(1), 80-84.
  • Tadić, V. M., Jeremic, I., Dobric, S., Isakovic, A., Markovic, I., Trajkovic, V., & Arsic, I. (2012). Anti-inflammatory, gastroprotective, and cytotoxic effects of Sideritis scardica extracts. Planta medica, 78(05), 415-427.
  • Tomás-Barberán F., Reijdali M, Harborne J B & Heywood V H (1988). External and vacuolar flavonoids from the Ibero- North African Sideritis species. A chemosystematic approach. Phytochemistry 27: 165- 170.
  • Yumrutas, O., Oztuzcu, S., Pehlivan, M., Ozturk, N., Poyraz, I. E., Iğci, Y. Z., ... & Arslan, A. (2015). Cell viability, anti-proliferation and antioxidant activities of Sideritis syriaca, Tanacetum argenteum subsp. argenteum and Achillea aleppica subsp. zederbaueri on human breast cancer cell line (MCF-7).
  • Zarzuelo A, Garcia E, Jimenez J, Ocete MA, Utrilla P (1993): Anti-inflamatory and anti-ulcerative activity of various species of the genus Sideritis from the Alpujarra region of Spain. Fitoterapia 64: 26–30.
There are 22 citations in total.

Details

Primary Language English
Journal Section First Issue
Authors

Ela Nur Şimşek Sezer

Tuna Uysal

Publication Date July 28, 2018
Published in Issue Year 2018 Volume: 1 Issue: 1

Cite

APA Şimşek Sezer, E. N., & Uysal, T. (2018). THE EFFECTS OF THE SIDERITIS OZTURKII EXTRACT ON THE EXPRESSION LEVELS OF SOME APOPTOTIC GENES. Current Perspectives on Medicinal and Aromatic Plants, 1(1), 8-12.

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