Year 2021, Volume 4 , Issue 1, Pages 22 - 35 2021-05-02

Phytochemical screening, Free radical scavenging, and In-vitro Anti-bacterial activity study of Chloroform, Acetone and Methanol extracts of selected medicinal plants of Nepal

Rishiram BARAL [1] , Amrit KARKI [2] , Saurav KARKI [3] , Bhuvan NEUPANE [4] , Pratigya KOIRALA [5] , Seema BARAL [6] , Sushil PANTA [7]

Bacteria are unique prokaryotic agents which carry genetic information in double stranded DNA matrix and even reside in the normal flora of human. Such bacteria sometime become the cause of human infection and disease by evading bodies protective mechanisms. Antibacterial agents are the group of materials that fight against such pathogenic bacteria, killing or reducing their metabolic activity. Meanwhile, naturally occurring plant phytochemicals are being used as antimicrobial and antibacterial agents by various group of indigenous and ethnic populations. This study was carried out to extra plot the safety, efficacy and therapeutic potency of the selected medicinal plants which are used traditionally as a source of anti-microbial, anti-bacterial and free radical scavenger. Quantitative phytochemical screening revealed that methanolic extract of Crassocephalum cripidiodes showed maximum Total Phenolic Content (TPC) of 322.16±0.01 μg GAE/mg of extract and acetone extract of Ficus. semicordata showed maximum Total Flavonoid Content (TFC) of 500.35±0.048 μg QE/mg of extract. Among the selected plant extracts, the most potent antioxidant activity was revealed by methanolic extract of Crassocephalum crepidiodes with IC50 of 6.95µg which is close to that of standard ascorbic acid having IC50 4.21µg. Bacterial susceptibility assay was carried out against Staphylococcus aureus, Klebsiella pneumonia and Pseudomonas aeruginosa. Among the selected plants, greater zone of inhibition was shown by acetone extract of Ficus semicordata against Pseudomonas aeruginosa. Finally, the study revealed that Ficus semicordata, Cirsium argyracanthum and Ficus hispida have anti-bacterial property against the selected bacterial strain which provide evidence-based scientific proof towards the traditional use of these plant sample in curing bacterial diseases.
Ethnomedicinal plants, phenols, flavonoids, Free radical scavenging activity, Antibacterial activity
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Primary Language en
Subjects Pharmacy
Journal Section Research Articles

Orcid: 0000-0003-3701-1873
Author: Rishiram BARAL (Primary Author)
Institution: Kyungpook National University
Country: Nepal

Orcid: 0000-0002-0331-4350
Author: Amrit KARKI
Institution: Pokhara University
Country: Nepal

Orcid: 0000-0002-0422-7697
Author: Saurav KARKI
Institution: Pokhara University
Country: Nepal

Orcid: 0000-0002-6469-1117
Author: Bhuvan NEUPANE
Institution: Pokhara University
Country: Nepal

Orcid: 0000-0002-5653-287X
Author: Pratigya KOIRALA
Institution: Pokhara University
Country: Nepal

Orcid: 0000-0002-0615-8680
Author: Seema BARAL
Institution: Pokhara University
Country: Nepal

Orcid: 0000-0002-8401-4471
Author: Sushil PANTA
Institution: Pokhara University
Country: Nepal


Publication Date : May 2, 2021

APA Baral, R , Karkı, A , Karkı, S , Neupane, B , Koırala, P , Baral, S , Panta, S . (2021). Phytochemical screening, Free radical scavenging, and In-vitro Anti-bacterial activity study of Chloroform, Acetone and Methanol extracts of selected medicinal plants of Nepal . Current Perspectives on Medicinal and Aromatic Plants (CUPMAP) , 4 (1) , 22-35 . DOI: 10.38093/cupmap.896273