Biological screening of Pleurostylia opposita (Wall.) Alston. – a rare medicinal plant of Eastern Ghat

Year 2022, Volume: 5 Issue: 2, 162 - 171, 02.01.2023

Mahendra C

https://doi.org/10.38093/cupmap.1208435

Abstract

The present study aims to evaluate the phytochemical, antioxidant and antibacterial properties in Pleurostylia opposita in various solvent crude extracts of leaf and bark. Preliminary phytochemical and antibacterial screening by well diffusion method was conducted. Further, total phenolic, and flavonoid contents estimation along with antioxidants properties by DPPH method was carried out in various solvent extracts. The phytochemical study revealed that the maximum secondary metabolites such as alkaloids, polyphenols, sterols, terpenoids flavonoids, and saponins were present in methanol extracts followed by other extracts. Likewise, antibacterial screening showed the maximum growth of inhibition in methanol leaf and bark extracts against E. coli (23mm), Salmonella typhi (23mm), Bacillus subtilis (24mm), Staphylococcus aureus (19mm), and Pseudomonas aeroginosa (24mm). The minimum inhibitory concentrations of the leaf and bark extracts were in the range of 19.5 mg/L - 625mg/L while gentamycin is 9.75mcg/L. The results indicate that the methanol extracts of leaf and bark showed potent antibacterial efficacy. Further, the antioxidant activity by DPPH assay indicates, that all the extracts exhibited significant antioxidant properties in a dose-dependent manner. Among the tested extracts, methanol exhibited excellent radical scavenging activity which was significant compared to Gallic acid. Likewise, each extract also showed excellent sources of phenolic and flavonoid contents. This study reveals that the plant contains a considerable amount of phenols and flavonoids, including antioxidant properties which are also possessing antibacterial efficacy. This study offered a new avenue for the potential use of this species as a source of natural antioxidants.

Keywords

Pleurostylia opposita, Phytochemical, Antibacterial, Antioxidants, DPPH.

Supporting Institution

University of Mysore

Project Number

Nil

Thanks

We are thankful to the Department of Studies in Botany, University of Mysore for providing the laboratory facility

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