The role of stem cell markers in choriocarcinoma: Immunocytochemical analysis in JAR cell line

H. Seda Vatansever

The role of stem cell markers in choriocarcinoma: Immunocytochemical analysis in JAR cell line

Abstract

Objective: Choriocarcinoma is a type of cancer that originates from placental trophoblastic tissue and has a high malignant potential. The aim of this study was to investigate the distribution of stem cell and cancer stem cell markers in the JAR cell line. The study is to provide information about the biology of choriocarcinoma and the mechanisms of resistance to treatment. Materials and Methods: JAR cells were cultured in RPMI 1640 culture medium. Colony formation assay was performed in JAR cells. The expression of OCT3/4, CD133, C-KIT, SALL4 and CD90 markers were analysed using indirect immunoperoxidase method. Healthy endometrial epithelial cell line RL95-2 was used for statistical analysis. Results: JAR cells showed statistically high colony formation efficiency compared to the RL95-2 group. Immunocytochemical analyses showed strong immunoreactivity for CD133 and moderate immunoreactivity for SALL4. C-KIT, OCT3/4 and CD90 showed weak immunoreactivity. Conclusion: The JAR cell line has emerged as a valuable model for trophoblastic cell invasion and placentation studies in choriocarcinoma. Markers such as CD133 and SALL4 provide important information about stem cell-like characteristics and choriocarcinoma malignancy. Lower expressions of C-KIT, OCT3/4 and CD90 reflect heterogeneous stem cell profiles in JAR cells. This diversity provides important insights into the complex nature of choriocarcinoma stem cells and sheds light on potential therapeutic approaches. Keywords: Choriocarcinoma, JAR cell line, stem cell markers, cancer stem cells, trophoblastic malignancy.

Keywords

References

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Details

Primary Language

English

Subjects

Clinical Oncology

Journal Section

Research Article

Authors

H. Seda Vatansever
0000-0002-7415-9618
Türkiye

Publication Date

March 12, 2025

Submission Date

October 12, 2024

Acceptance Date

January 2, 2025

Published in Issue

Year 2025 Volume: 39 Number: 1

IZ

https://izlik.org/JA39HX29UR

Cite

RIS / Bibtex

APA

Vatansever, H. S. (2025). The role of stem cell markers in choriocarcinoma: Immunocytochemical analysis in JAR cell line. Developments and Experiments in Health and Medicine, 39(1), 23-30. https://izlik.org/JA39HX29UR

AMA

1.Vatansever HS. The role of stem cell markers in choriocarcinoma: Immunocytochemical analysis in JAR cell line. Dev Exp Health Med. 2025;39(1):23-30. https://izlik.org/JA39HX29UR

Chicago

Vatansever, H. Seda. 2025. “The Role of Stem Cell Markers in Choriocarcinoma: Immunocytochemical Analysis in JAR Cell Line”. Developments and Experiments in Health and Medicine 39 (1): 23-30. https://izlik.org/JA39HX29UR.

EndNote

Vatansever HS (March 1, 2025) The role of stem cell markers in choriocarcinoma: Immunocytochemical analysis in JAR cell line. Developments and Experiments in Health and Medicine 39 1 23–30.

IEEE

[1]H. S. Vatansever, “The role of stem cell markers in choriocarcinoma: Immunocytochemical analysis in JAR cell line”, Dev Exp Health Med, vol. 39, no. 1, pp. 23–30, Mar. 2025, [Online]. Available: https://izlik.org/JA39HX29UR

ISNAD

Vatansever, H. Seda. “The Role of Stem Cell Markers in Choriocarcinoma: Immunocytochemical Analysis in JAR Cell Line”. Developments and Experiments in Health and Medicine 39/1 (March 1, 2025): 23-30. https://izlik.org/JA39HX29UR.

JAMA

1.Vatansever HS. The role of stem cell markers in choriocarcinoma: Immunocytochemical analysis in JAR cell line. Dev Exp Health Med. 2025;39:23–30.

MLA

Vatansever, H. Seda. “The Role of Stem Cell Markers in Choriocarcinoma: Immunocytochemical Analysis in JAR Cell Line”. Developments and Experiments in Health and Medicine, vol. 39, no. 1, Mar. 2025, pp. 23-30, https://izlik.org/JA39HX29UR.

Vancouver

1.H. Seda Vatansever. The role of stem cell markers in choriocarcinoma: Immunocytochemical analysis in JAR cell line. Dev Exp Health Med [Internet]. 2025 Mar. 1;39(1):23-30. Available from: https://izlik.org/JA39HX29UR