Investigation of genetic diversity of different accessions of resistance against Phytophthora capsici L. using SRAP and SSR markers

Abstract

Phytophthora capsici L. is one of the most important pathogens of pepper in the world. Different genetic resources that resistant to P. capsici have been reported so far. Determination of the phylogenetic relationships of resistant pepper genotypes is important for transferring of resistance gene/genes to cultivated varieties. In this study, 12 pepper genotypes from different accessions of resistance and 4 susceptible genotypes tested with P. capsici were identified genetic relationship among genotypes using 144 SRAP (Sequence Related Amplified Polymorphism) and 27 SSR (Simple Sequence Repeat) primer combinations. 31 SRAP primers were polymorphic and yielded 254 polymorphic DNA fragments. Fifty SRAP primers were only monomorphic. However, 32 SRAP primers were not produced PCR products. Results showed that 16 pepper genotypes were genetically separated from each other by SRAP primers. Twelve SSR primers yielded total 36 DNA fragments, 33 of these were polymorphic (93%). However 15 SSR primers only produced monomorphic DNA bands. Results showed that Pepper genotypes PM-217 KM2-11, Perennial LS-279, PBC-178, Sera Demre, PBC 179 and KMAE-12 were not separated with SSR markers. When the SRAP and SSR marker data were evaluated together, the results were more informative about the genetic relationship of genotypes and clustering by origin. Findings indicated that the genotype CM 334 which is resistance resource to P. capsici was genetically different from PM 702, Perennial, PM-217, Taiwan's group called as PBC label (1364, 1365, 178, 179 and 413) and KM211 selected from Kahramanmaraş pepper populations.

Keywords

• Pepper,Phytophthora capsici L.,SRAP,SSR,Genetic relationship

Phytophthora capsici'ye dayanıklı bazı biber genotiplerinin SRAP ve SSR belirteçlerle genetik farklılıklarının belirlenmesi

Abstract

Dünyada biber yetiştiriciliğini sınırlayan en önemli hastalıklardan birisi, kök boğazı yanıklığıdır. Bazı başka türlerde olduğu gibi Capsicum annuum L. türüne ait farklı genotiplerde de etmene karşı dayanıklılık kaynakları mevcuttur. Dayanıklılık düzeyi farklı biber genotiplerinin genetik ilişkilerinin belirlenmesi, dayanıklılık ile ilgili gen/genlerin kültür çeşitlerine aktarılması açısından önemlidir. Bu çalışmada, P. capsici’ye karşı dayanıklılık düzeyi ve orijini farklı 12 dayanıklı ve 4 duyarlı biber genotipin filogenetik ilişkileri SRAP (Sequence Related Amplified Polymorphism) ve SSR (Simple SequenceRepeat) moleküler belirteçleriyle belirlenmiştir. Bunun için, 144 SRAP primer kombinasyonu ve 27 SSR primer çifti kullanılmıştır. Otuz iki SRAP primeri herhangi bir PCR ürünü vermemiştir. Elli primer kombinasyonunda yalnızca monomorfik DNA bantı oluşurken 31 primer kombinasyonu polimorfizm sağlamış toplam 254 DNA fragmenti elde edilmiş, bunların, 99’u (%39) monomorfik, 155’i (%61) polimorfik DNAbandı olarak değerlendirilmiştir. SRAP belirteçleri ile 16 biber genotipi birbirinden genetik olarak ayrılmıştır. SSR primerinin 15’i monomorfik bant oluşturmasına karşın 12 SSR primeri ile toplam 36 DNA bandı elde edilmiş bunların 33'ü (%93) polimorfik olmuştur. SSR belirteç sistemi ile 16 biber genotipinin bazıları (PM-217 KM2-11, Perennial LS-279, PBC-178, Sera Demre, PBC 179 KMAE-12) birbirinden ayrılamamıştır. SRAP ve SSR belirteç verileri birlikte değerlendirildiğinde, genotiplerin genetik ilişkisi, orijine göre kümelemesi daha bilgi verici olmuştur. P. capsici’ye karşı dayanıklılık genitörü olarak kullanılan CM 334'den, PM 702, Perennial, PM-217, Tayvan'da temin edilen PBC grubu (1364, 1365, 178, 179 ve 413) ile Kahramanmaraş biber populasyonundan selekte edilen KM211genotipleri genetik olarak farklı gruplarda yer aldığı belirlenmiştir.

Keywords

• Biber,kök boğazı yanıklığı,SRAP,SSR,Genetik ilişki

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