The effect of arachidonic acid elicitor on phytoalexin stimulation in eggplant callus suspension culture

Abstract

The aim of this study was to investigate the relationship between the resistance of Verticillium wilt and the amount of phytoalexin, which is the defense molecules synthesized in plant tissues. In this study, Long Purple eggplant variety (disease susceptible) and S. sisymbriifolium wild type (disease resistant) were used as plant material. As elicitor, arachidonic acid (5 and 10 mM) was included in the study. Stimulation with elicitor lasted 24, 48 and 72 hours. Extracted substances were analyzed in gas liquid chromatography (GLC). The formation of phytoalexin was not adequately stimulated in the callus suspension culture of the resistant species. The results showed that arachidonic acid was effective in stimulating the formation of phytoalexins in the callus suspension culture in Long Purple variety, and in the in vitro conditions solavetivone was formed in the tissue phase and lubimin substances in the filtrate phase. The highest material accumulations were obtained in both phases: ’10 mM arachidonic acid x 72 hours’ ‘elicitor dose x stimulation period’ interaction was found to be significant.

Keywords

• Callus culture,Eggplant,Lubimin,Resistance,Solavetivone

Araşidonik asidin patlıcan kallus süspansiyon kültüründe fitoaleksin uyartımı üzerine etkisi

Abstract

Bu çalışmanın amacı; Verticillium solgunluğuna dayanımın, bitki dokularında sentezlenen fitoaleksin miktarıyla ilişkisini araştırmaktır. Çalışmada Long Purple patlıcan çeşidi (hastalığa duyarlı) ile S. sisymbriifolium yabani türü (hastalığa dayanıklı) bitkisel materyal olarak kullanılmıştır. Elisitör olarak, araşidonik asit (5 ve 10 mM) çalışmada yer almıştır. Elisitör ile uyarım işlemi 24, 48 ve 72 saat olarak uygulanmıştır. Ekstrakte edilen maddeler gaz likit kromotografisi (GLC) cihazında analiz edilmiştir. Dayanıklı türün kallus süspansiyon kültüründe fitoaleksin oluşumu yeterli düzeyde uyarılamamıştır. Elde edilen bulgular araşidonik asidin kallus süspansiyon kültüründe sadece Long Purple çeşitinde fitoaleksinlerin oluşumunu uyarmada etkili olduğu, in vitro koşularda doku fazında solavetivon ve süzüntü fazında ise lubimin maddelerinin biriktiği belirlenmiştir. En yüksek madde birikimleri her iki fazda ‘10 mM araşidonik asit x 72 saat uyarı süresi’ kombinasyonunda elde edilmiş, ‘doz x uygulama süresi’ etkileşimi önemli bulunmuştur.

Keywords

• Dayanıklılık,Kallus kültürü,Lubimin,Patlıcan,Solavetivon

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