DIVERSITY AND RELATIONSHIPS OF PONCIRUS (Poncirus trifoliata L.) AND PONCIRUS HYBRIDS BY SSR MOLECULAR MARKERS

Abstract

In this study, genetic relationship and diversity were determined by SSR (simple sequence repeat) molecular markers technique among 59 Poncirus (Poncirus trifoliata L. Raf.) types/cultivars derived from selections, introduction and hybridization. In this work, 14 SSR primers (TAA1, CAC19, TAA45, CT21, CAC33, CAC39, TAA33, CCT01, CAG01, CAT01, ATC09, TAA52, TAA15 and CAGG9) of the used 26 SSR primers produced polymorphic fragments. Genetic relationship and distance were determined by using UPGMA (unweighted-pair group method with arithmetic average) and PCA (principal component analysis) analysis. The Dice’s similarity coefficient among Poncirus and their hybrids accessions ranged from 0.63 to 1.00 and matrix correlation was 0.84. While Poncirus Krides SRA 339 - M and Poncirus SEAB B 6C - BB1 (SRA) in a group; Poncirus Town (SRA) and Poncirus Jacobsen B 6C - Z19 (SRA) in a different group; Poncirus Dwarf (SRA) and Poncirus Yamaguchi B 6C - Z28 (SRA) in a different group with 100% genetic similarity were determined. On the contrary, Glenn citrangedin (CRC 3573) 63% genetic similarity is the farthest among 60 Poncirus.

Keywords

• Poncirus trifoliata L.; SSR; Genetic resource; Molecular marker

ÜÇ YAPRAKLI (Poncirus trifoliata L.) VE ÜÇ YAPRAKLI MELEZLERİ GRUBU TURUNÇGİLLERİN GENETİK AKRABALIK VE FARKLILIKLARININ SSR MOLEKÜLER MARKIRLARLA TANIMLANMASI

Abstract

Çalışmada, seleksiyon, introduksiyon ve melezlemelerle elde edilmiş 59 adet üç yaprak tip/çeşidinin genetik farklılığı ve birbiriyle olan genetik yakınlığı SSR (simple sequence repeat) moleküler markır tekniği kullanılarak incelenmiştir. Kullanmış olduğumuz 26 adet SSR primerinden 14 tanesi (TAA1, CAC19, TAA45, CT21, CAC33, CAC39, TAA33, CCT01, CAG01, CAT01, ATC09, TAA52, TAA15 ve CAGG9) polimorfik bulunmuştur. UPGMA (unweighted-pair group method with aritmetic average) ve PCA (principal component analysis) analizleri sonucu bireylerin birbirleriyle olan genetik yakınlıkları ve uzaklıkları belirlenmiştir. Dice’ın benzerlik katsayısına göre benzerlik oranları 0.63-1.00 arasında değişim göstermiş, matriks korelasyon katsayısı 0.84 olarak bulunmuştur. Benzerlik oranı Poncirus Krides SRA 339 ile Poncirus SEAB B 6C - BB1 (SRA); Poncirus Town (SRA) ile Poncirus Jacobsen B 6C - Z19 (SRA); Poncirus Dwarf (SRA) ile Poncirus Yamaguchi B 6C - Z28 (SRA) arasında ve 1.00 (% 100) oranında olduğu tespit edilmiştir. Glenn citrangedin (CRC 3573) ise % 63 oranıyla 60 birey içerisinden en uzak bireyi oluşturmaktadır.

Keywords

• Poncirus trifoliata L., SSR, Genetik kaynaklar, Moleküler markır

References

1. Barkley, N.A., Roose, M.L., Krueger, R.R., Federici, C.T. 2006. Assessing Genetic Diversity and Population Structure in a Citrus Germplasm Collection Utilizing Simple Sequence Repeat Markers (SSRs), Theor Appl Genet, 112: 1519–1531.
2. Bretó, M.P., Ruiz, C., Pina, J.A., Asíns, M.J. 2001. The diversification of Citrus clementina Hort. ex Tan., a vegetatively propagated crop species. Molecular Phylogenetics and Evolution 21:285-93.
3. Chen, C., Bowman, K.D., Choi, Y.A., Dang, P.M., Rao, M.N., Huang S., Soneji, J.R., McCollum, T.G., Gmitter, F.G. 2008. EST-SSR Genetic Maps For Citrus Sinensis And Poncirus Trifoliata. Tree Genetics & Genomes 4:1–10
4. Corazza-Nunes, M.J., Machado, M.A., Nunes, W.M.C., Cristofani, M., Targon, M.L.P.N. 2002. Assessment of Genetic Variability in Grapefruits (C. paradisi Macf.) and Pummelos (C. maxima(Burm.) Merr.) Using RAPD and SSRs Markers. Euphytica 126:169-76.
5. Dice, L.R., 1945. Measures of The Amount of Ecologic Association Between Species. Ecology 26:297-302.
6. Fang, D.Q., Roose, M.L., Krueger, R.R., Federici, C.T. 1997. Fingerprinting Trifoliate Orange Germplasm Accessions with Isozymes, RFLPs, and Inter-simple Sequence Repeat Markers. Theor Appl Genet 95:211–219
7. Jiang, D., Zhong, G-Y., Hong, Q-B. 2006. Analysis of Microsatellites in Citrus Unigenes. Acta Genetica Sinica, 33 (4): 345-353
8. Novelli, V.M., Cristofan, M., Souza, A.A., Marcos, A., Machado, M.A. 2006. Development and Characterization of Polymorphic Microsatellite Markers for The Sweet Orange (Citrus sinensis L. Osbeck). Genetics and Molecular Biology, 29, 1, 90-96.

9. Rohlf FJ., 1993. NTSYS-PC, Numerical Taxonomy and Multivariate Analysis System. Version 1.80. Exeter Software, Setauket, New York.
10. Roose, ML., 2009. Use of Molecular Markers to Understand Phylogeny and Genetic Diversity of Citrus. PCR Primers for Citrus Germplasm Characterization. http://www.plantbiology.ucr.edu/faculty/roose.html.
11. Soost, R.K., Cameron, J.W. 1975. Citrus. In: Janick J, Moore JN (eds) Advances in fruit breeding. Purdue University,West Lafayette, Indiana,USA,507–540
12. Şahin Çevik M., Moore, G.A. 2007. Construction of a Genetic Linkage Map of Citrus with Random Amplified Polymorphic DNA (RAPD) Markers Using a Progeny Population from a Complex Intergeneric Cross. Turk J Bot 31 79-86.
13. Tan, M-l., Song, J-K., Deng, X-X. 2007. Production of Two Mandarin Trifoliate Orange Hybrid Populations Via Embryo Rescue with Verification by SSR Analysis. Euphytica 157:155–160.
14. Tuzcu, O., Erkan. O., Ozsan, M. 1976. Turunçgil Fidanı Üreten İşletmelerimizin Teknik ve Ekonomik Faaliyetleri Üzerinde Bir Araştırma. Ç.Ü. Ziraat Fakültesi Yayınları: 128, Ankara Üniversitesi Basımevi, Adana. 72 s.
15. Tuzcu, Ö., Yesiloglu, T., Yildirim B. 2001. Citrus 2001 Reports : Turkey. Florida Grower Annual Edition. Mid.
16. Uzun, A. 2002. Turunçgil Anaçları. Tarım ve Köyişleri Bakanlığı. Tarımsal Araştırmalar Genel Müdürlüğü, ALATA Bahçe Kültürleri Araştırma Enstitüsü Yayınları.
17. Weber, C.A., Moore, G.A., Deng, Z., Gmitter, F.G. 2003. Mapping Freze Tolerance Quantitative Trait Loci in a Citrus grandis x Poncirus trifoliata F1 pseudo- testcross Using Molecular Markers. J Amer Soc Hort Sci 128: 508-514.