An evergreen host for Cucumber Mosaic Virus (CMV) in Turkey: Polygala myrtifolia

Year 2017, Volume: 34 Issue: 1, 29 - 36, 01.06.2017

Gökmen Koç , Hakan Fidan

https://doi.org/10.16882/derim.2017.305281

Abstract

Polygala myrtifolia is
known as Myrtle-leaf milkwort or Petite Butterflys, which is cultivated as an
ornamental in Turkey. During the observations in 2014-2015 summers, systemic
mosaic and irregular yellowish lines were recorded on leaves. In order to
identify causal agent, leaf samples were tested by DAS-ELISA using specific
polyclonal antiserums against to TRSV (Tobacco
Ringspot Virus), ToRSV (Tomato
Ringspot Virus), SLRSV (Strawberry
Latent Ringspot Virus), ArMV (Arabis
Mosaic Virus), CMV (Cucumber Mosaic
Virus), TMV (Tobacco Mosaic Virus),
TNV (Tobacco Necrosis Virus), ToMV (Tomato Mosaic Virus), Tospo (Tospovirus), TSWV (Tomato Spotted Wilt Virus), PVX (Potato Virus X), SqMV (Squash
Mosaic Virus), Poty (Group Poty Virus),
PVY (Potato Virus Y), AMV (Alfalfa Mosaic Virus) and ZYMV (Zucchini Yellow Mosaic Virus), supplied
by BIOREBA AG for common ornamental plant viruses. The samples (8 individual,
13.1%) which are positive for CMV in DAS-ELISA, were confirmed by RT-PCR using
total RNA extracted from leaves (100 mg). 513 bp cDNA bands in RT-PCR assay by
primer pairs Sense CMVr and Antisense CMVf were observed in 1.5% agarose gel.
In Turkey, this study is the first record of CMV via serologicaly and moleculer
technics on Polygala that is evergreen in Antalya, may be also evidence of
uncontrolled distribution between species and vectors.

Keywords

CMV, DAS-ELISA, Polygala myrtifolia, RT-PCR, Turkey

Türkiye’de Cucumber Mosaic Virus (CMV) için herdem yeşil bir konukçu: Polygala myrtifolia

Abstract

Mersin Yapraklı Sütotu ya da Küçük Kelebek, olarak
bilinen Polygala myrtifolia,
Türkiye’de süs bitkisi olarak yetiştirilmektedir. 2014-2015 yaz mevsimlerinde
yapılan gözlemler sırasında Mersin Yapraklı Sütotu bitkilerinin yapraklarında,
sistemik mozaik ve düzensiz sarımsı izler gözlenmiştir. Neden olan etmenin
tanılanma için yaprak örnekleri BIOREBA AG’dan (Switzerland) temin edilen
spesifik poliklonal antiserum ile DAS-ELISA tekniğine göre TRSV (Tobacco Ringspot Virus), ToRSV (Tomato Ringspot Virus), SLRSV (Strawberry Latent Ringspot Virus), ArMV
(Arabis Mosaic Virus), CMV (Cucumber Mosaic Virus), TMV (Tobacco Mosaic Virus), TNV (Tobacco Necrosis Virus), ToMV (Tomato Mosaic Virus), Tospo (Tospovirus), TSWV (Tomato Spotted Wilt Virus), PVX (Potato Virus X), SqMV (Squash
Mosaic Virus), Poty (Group Poty Virus),
PVY (Potato Virus Y), AMV (Alfalfa Mosaic Virus) ve ZYMV (Zucchini Yellow Mosaic Virus) gibi
bilinen süs bitkisi virüslerine karşı testlenmiştir. DAS-ELISA’da CMV pozitif
örnek (8 adet, %13.1) sonuçları, 100 mg yapraktan ekstrakte edilen toplam
RNA’ların RT-PCR’da kullanılmasıyla doğrulanmıştır. Sense CMVr ve Anti sense
CMVf primerleriyle yapılan RT-PCR sonucu, 513 bp cDNA bandları %1.5’lik agaroz
jelde gözlenmiştir. Antalya’da her dem yeşil olan Sütotu’nda CMV’nin serolojik
ve moleküler tekniklerle ilk kaydı olan bu çalışma belki de türler ve bölgeler
arasında kontrolsüz yayılımının kanıtıdır.

Keywords

CMV, DAS-ELISA, Polygala myrtifolia, RT-PCR, Türkiye

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