Determination of polycyclic aromatic hydrocarbons in butter and vegetable oils available in Turkish markets

Year 2019, Volume: 36 Issue: 2, 177 - 182, 10.12.2019

S. Sezer Kıralan , İsra Toptancı

https://doi.org/10.16882/derim.2019.580858

Abstract

This study was conducted to determine the content of PAH4
(benz[a]anthracene, chrysene, benzo[b]fluoranthene and benzo[a]pyrene) in butters
and vegetable oils (olive oil, palm oil, sunflower oil, almond oil, hazelnut
oil and coffee oil) sold in Turkish local markets. A series of 15 vegetable
oils and butters collected from markets were analyzed in the study. Among
vegetable oils, sunflower oil presented highest amount of PAH4 (20.76 µg kg^-1)
followed by olive oil (9.51 µg kg^-1), palm oil
(6.05 µg kg^-1), coffee oil (5.25 µg kg^-1)
and almond oil (1.95 µg kg^-1), respectively. PAH4 was not
detected for butters among analysed samples. As a result, PAH4 can occur in
various vegetable oils at different levels and PAH4 levels detected in all oil
samples except sunflower oil were below the Turkish Food Codex.

Keywords

PAH4, Butter, Vegetable oil, Market survey

Türkiye piyasasında satışa sunulmuş olan bitkisel yağlar ve tereyağlarında polisiklik aromatik hidrokarbon miktarının tespit edilmesi

Abstract

Bu çalışma, Türkiye piyasasında satışa
sunulan tereyağları ve bitkisel yağların (zeytinyağı, palm yağı, ayçiçek yağı,
badem yağı, fındık yağı ve kahve yağı) PAH4 (benz[a]antrasen, krisen,
benzo[b]fluoranthene ve benzo[a]piren) miktarlarını belirlemek amacıyla
yürütülmüştür. Çalışmada marketlerden temin edilen 15 adet bitkisel yağ ve
tereyağı analiz edilmiştir. Analiz edilen bitkisel yağ örneklerinde en yüksek
PAH4 miktarı (20.76 µg kg^-1) ayçiçek yağında tespit
edilmiş olup, bunu sırasıyla zeytinyağı (9.51 µg kg^-1),
palm yağı (6.05 µg kg^-1), kahve yağı (5.25 µg kg^-1)
ve badem yağı (1.95 µg kg^-1) izlemiştir. Analiz edilen
tereyağlarında PAH miktarı tespit edilmemiştir. Sonuç olarak, Türkiye
piyasasında yer alan çeşitli bitkisel yağlarda farklı miktarlarda PAH4
bulunabilmektedir ve ayçiçek yağı dışındaki örneklerin PAH4 miktarları Türk
Gıda Kodeksi limit değerinin altında olduğu tespit edilmiştir.

Keywords

PAH4, Tereyağı, Bitkisel yağ, Pazar araştırması

References

• Alomirah, H., Al-Zenki, S., Husain, A., Sawaya, W., Ahmed, N., Gevao, B., & Kannan, K. (2010). Benzo [a] pyrene and total polycyclic aromatic hydrocarbons (PAHs) levels in vegetable oils and fats do not reflect the occurrence of the eight genotoxic PAHs. Food Additives & Contaminants, 27(6):869-878.
• Baloğlu, Z., & Bayrak, A. (2006). Polisiklik aromatik hidrokarbon (PAH)'lardan benzo (A) pirenin sızma, riviera ve prina zeytinyağlarında belirlenmesi. Gıda, 31(5): 239-251.
• Barranco, A., Alonso-Salces, R.M., Bakkali, A., Berrueta, L.A., Gallo, B., Vicente, F., & Sarobe, M. (2003). Solid-phase clean-up in the liquid chromatographic determination of polycyclic aromatic hydrocarbons in edible oils. Journal of Chromatography A, 988(1):33-40.
• Cejpek, K., Hajšlová, J., Kocourek, V., Tomaniova, M., & Cmolik, J. (1998). Changes in PAH levels during production of rapeseed oil. Food Additives & Contaminants, 15(5):563-574.
• Dennis, M. J., Massey, R. C., Cripps, G., Venn, I., Howarth, N., & Lee, G. (1991). Factors affecting the polycyclic aromatic hydrocarbon content of cereals, fats and other food products. Food Additives & Contaminants, 8(4):517-530.
• EFSA (2006). Setting maximum levels for certain contaminants in food stuffs. https://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2006:364:0005:0024. Erişim tarihi:20.06.2019.
• Ergönül, P.G., & Sánchez, S. (2013). Evaluation of polycyclic aromatic hydrocarbons content in different types of olive and olive pomace oils produced in Turkey and Spain. European Journal of Lipid Science and Technology, 115(9):1078-1084.
• Fromberg, A., Højgård, A., & Duedahl-Olesen, L. (2007). Analysis of polycyclic aromatic hydrocarbons in vegetable oils combining gel permeation chromatography with solid-phase extraction clean-up. Food Additives & Contaminants, 24(7):758-767.
• Hu, C., He, M., Chen, B., Zhong, C., & Hu, B. (2014). Sorptive extraction using polydimethylsiloxane/metal–organic framework coated stir bars coupled with high performance liquid chromatography-fluorescence detection for the determination of polycyclic aromatic hydrocarbons in environmental water samples. Journal of Chromatography A, 1356: 45-53.
• Hopia, A., Pyysalo, H., & Wickström, K. (1986). Margarines, butter and vegetable oils as sources of polycyclic aromatic hydrocarbons. Journal of the American Oil Chemists’ Society, 63(7):889-893.
• Jira, W. (2004). A GC/MS method for the determination of carcinogenic polycyclic aromatic hydrocarbons (PAH) in smoked meat products and liquid smokes. European Food Research and Technology, 218(2):208-212.
• Kiralan, S.S., Erdogdu, F., & Tekin, A. (2017). Reducing polycyclic aromatic hydrocarbons (PAHs) formation in olive pomace oil using microwave pre‐heating of olive pomace. European Journal of Lipid Science and Technology, 119(1):600241.
• Kiralan, S.S., Toptancı, İ., & Tekin, A. (2019). Further evidence on the removal of polycyclic aromatic hydrocarbons (PAHs) during refining of olive pomace oil. European Journal of Lipid Science and Technology, 121(4):1800381.
• Martorell, I., Perelló, G., Martí-Cid, R., Castell, V., Llobet, J.M., & Domingo, J. L. (2010). Polycyclic aromatic hydrocarbons (PAH) in foods and estimated PAH intake by the population of Catalonia, Spain: temporal trend. Environment International, 36(5):424-432.
• Miao, J., Che, K., Xi, R., He, L., Chen, X., Guan, X., Zhuang, X., Wen, X. & Cao, Y. (2013). Characterization and benzo [a] pyrene content analysis of camellia seed oil extracted by a novel subcritical fluid extraction. Journal of the American Oil Chemists' Society, 90(10):1503-1508.
• Moret, S., & Conte, L. S. (2002). A rapid method for polycyclic aromatic hydrocarbon determination in vegetable oils. Journal of Separation Science, 25(1‐2):96-100.
• Moret, S., Purcaro, G., & Conte, L.S. (2005). Polycyclic aromatic hydrocarbons in vegetable oils from canned foods. European Journal of Lipid Science and Technology, 107(7‐8):488-496.
• Olsson, P., Sadiktsis, I., Holmbäck, J., & Westerholm, R. (2014). Class separation of lipids and polycyclic aromatic hydrocarbons in normal phase high performance liquid chromatography–A prospect for analysis of aromatics in edible vegetable oils and biodiesel exhaust particulates. Journal of Chromatography A, 1360:39-46.
• Petridis, N.P., Sakkas, V.A., & Albanis, T.A. (2014). Chemometric optimization of dispersive suspended microextraction followed by gas chromatography–mass spectrometry for the determination of polycyclic aromatic hydrocarbons in natural waters. Journal of Chromatography A, 1355:46-52.
• Potočnik, T., & Košir, I.J. (2017). Influence of roasting temperature of pumpkin seed on PAH and aroma formation. European Journal of Lipid Science and Technology, 119(3):1500593.
• SFC (2002): Opinion of the Scientific Committee on Food on the risks to human health of Polycyclic Aromatic Hydrocarbons in food. http://www.ec.europa.eu/food/sites/food/files/safety/docs/sci-com_scf_out153_en.pdf. Erişim tarihi: 20.06.2019.
• Şekeroğlu, G., Fadıloğlu, S., & Göğüş, F. (2006). Bitkisel yağlarda benzo (a) piren miktarinin yüksek basinçli sivi kromatografisi ile belirlenmesi. Türkiye, 9:24-26.
• Teixeira, V.H., Casal, S., & Oliveira, M.B.P. (2007). PAHs content in sunflower, soybean and virgin olive oils: Evaluation in commercial samples and during refining process. Food Chemistry, 104(1):106-112.
• TGK (2011). Türk Gıda Kodeksi Pestisitlerin Maksimum Kalıntı Limitleri Yönetmeliği. http://www.resmigazete.gov.tr/eskiler/2016/11/20161125M1-1.htm. Erişim Tarihi: 20.06.2019
• Van Der Wielen, J.C.A., Jansen, J.T.A., Martena, M.J., De Groot, H.N., & In't Veld, P.H. (2006). Determination of the level of benzo [a] pyrene in fatty foods and food supplements. Food Additives and Contaminants, 23(7):709-714.
• Viegas, O., Yebra-Pimentel, I., Martínez-Carballo, E., Simal-Gandara, J., & Ferreira, I.M. (2014). Effect of beer marinades on formation of polycyclic aromatic hydrocarbons in charcoal-grilled pork. Journal of Agricultural and Food Chemistry, 62(12):2638-2643.
• Welling, P., & Kaandorp, B. (1986). Determination of polycyclic aromatic hydrocarbons (PAH) in edible vegetable oils by liquid chromatography and programmed fluorescence detection comparison of caffeine complexation and XAD-2 chromatography sample clean-up. Zeitschrift für Lebensmittel-Untersuchung und Forschung, 183(2):111-115.
• Zhao, Q., Wei, F., Luo, Y. B., Ding, J., Xiao, N., & Feng, Y. Q. (2011). Rapid magnetic solid-phase extraction based on magnetic multiwalled carbon nanotubes for the determination of polycyclic aromatic hydrocarbons in edible oils. Journal of Agricultural and Food Chemistry, 59(24): 12794-12800.