Mass Production of Medicinal Plants for Obtaining Secondary Metabolite Using Liquid Mediums Via Bioreactor Systems: SETISTM And RITA®

Year 2018, Volume: 11 Issue: 2, 5 - 10, 27.12.2018

Ergun Kaya , Selin Galatalı Sevinç Güldağ Bilge Öztürk Muammer Ceylan Onur Çelik İrem Aktay

Abstract

Micropropagation techniques provide effective and important ways for
plant mass propagation. While some cultivated species are still accepted as
comparatively resistant to tissue culture, there are a lot of successful
applications of somatic and zygotic embriyogenesis, organaogenesis,
micropropagation have been reported from different type of plant species. In vitro liquid culture systems are
thought to be more efficient than semi-solid culture systems because of having
great advantages of them such as successful automation, big mass production,
easy handling, simple calibration of medium components and culture conditions.
Temporary immersion bioreactor systems (TIS) based on liquid medium usage have
been provided many utilities such as reduction of production cost and higher
proliferation rate. This review aimed to describe advantages of
micropropagation protocols developed using two different temporary immersion
bioreactor systems, SETIS^TM and RITA^®.

Keywords

Micropropagation, plant breeding, plant clonal propagation, TIS

Biyoreaktör Sistemleri Kullanılarak Sıvı Besiyeri Ortamında Sekonder Metabolit Üretimi için Tıbbi Bitkilerin Kitlesel Çoğaltımı: SETISTM And RITA®

Abstract

Mikroçoğlatım teknikleri, bitkilerin kitlesel çoğaltımı için etkin ve önemli bir yöntemdir. Bazı kültür çeşitleri hala doku kültürü yöntemleriyle çoğaltıma direnç gösterse de, somatik ve zigotik embriyogenez, organogenez, mikroçoğaltım gibi başarılı uygulamalar, farklı bitki türlerinin farklı çeşitlerinden rapor edilmiştir. Başarılı otomasyon, yüksek kitlesel üretim, kolay manupulasyon, kültür ortam bileşenlerinin basit kalibrasyonu gibi avantajlara sahip olması nedeniyle in vitro sıvı kültür sistemleri, yarı katı sistemlere göre çok daha etkin sistemlerdir. Sıvı besiyeri kullanımına dayalı periyodik daldırma biyoreaktör sistemleri (TIS), düşük üretim maliyeti ve yüksek çoğaltım oranı gibi faydalar sağlamaktadır. Bu derleme çalışması, SETIS^TM ve RITA^® gibi, iki farklı periyodik daldırma biyoreaktör sistemlerinin kullanımına dayalı geliştirilen mikroçoğaltım yöntemlerinin avantajlarını tanımlamayı amaçlamaktadır.

Keywords

Mikroçoğaltım, Bitki ıslahı, Bitki klonal çoğaltımı, TIS

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