Yaşlı Dişi Sıçanlarda Akut ve Kronik Stres Yanıtlarının Midede TNF-α ve IL-10 Düzeylerine Etkileri

Year 2019, , 225 - 233, 06.02.2020

Ferda Hoşgörler , Servet Kızıldağ Başar Koç Güven Güvendi Sevim Kandiş Mehmet Ateş Nazan Uysal

https://doi.org/10.5505/deutfd.2019.50490

Abstract

Amaç: Stres karşısında enerji ve kan akımını gereken bölgelere yönlendiren Hipotalamus-Pitüiter-Adrenal (HPA) aksın yanıtları yaşlılarda bozulmaktadır. Mide, stres ve kortisol etkilerine oldukça duyarlıdır. Akut stres ve kronik stres farklı kortisol cevapları oluşturabilir. Çalışmanın amacı yaşlı dişi sıçanlarda akut ve kronik stresin mide inflamasyon belirteçleri üzerine etkisinin değerlendirilmesidir.
Gereç ve Yöntem: 24-30 aylık dişi Sprague Dawley sıçanlar (n=18), kontrol, akut stres (60 dakikalık restraint stresi) ve kronik stres (14 gün tekrarlayan 60 dakikalık restraint stresi) gruplarında değerlendirildi. Stres sonrası serumda kortikosteron, mide dokularında TNF-α ve IL-10 düzeyleri ELISA yöntemi ile incelendi. Sonuçlar SPSS 24 programında One Way Anova ve post-hoc Tukey testi ile analiz edildi. Pearson korelasyon analiziyle kortikosteron düzeyleri ve sitokin düzeyleri arasındaki ilişki belirlendi, p<0,05 anlamlı kabul edildi.
Bulgular: Akut stres grubunda kontrol grubuna göre serum kortikosteron düzeylerinin arttığı, kronik stres grubunda kontrole göre değişmediği görüldü. Mide TNF-α düzeylerinin akut ve kronik stres grubunda kontrole göre arttığı görüldü. Akut stres ve kontrol gruplarında kortikosteron ve TNF-α arasında pozitif korelasyon saptandı. Mide IL-10 düzeylerinde gruplar arası belirgin bir fark saptanmadı.
Sonuç: Araştırmamızda yaşlı dişi sıçanlarda kronik stresin akut strese göre daha düşük kortikosteron yanıtı oluşturduğu ortaya koyulmuştur. Bu durum böbrek üstü bezinin glukokortikoid replasman yeteneğinin yaşlılarda yetersizliğiyle ilişkili olabilir. Tekrarlayan stresle akut inflamasyon bulgularının daha da artmaması, kronik strese karşı midenin adaptasyon geliştirme mekanizmalarını akla getirmiştir. Akut stresle ilişkili midedeki proinflamatuvar sitokin TNF-α düzeylerinin değişiminde kortikosteronun rolü olduğu ortaya konmuştur.

Keywords

yaşlı, mide, stres

Effects of Acute and Chronic Stress Responses on Gastric TNF-α and IL-10 Levels in Female Elderly Rats

Abstract

Objective: The physiological responses of the hypothalamus-pituitary-adrenal (HPA) axis, which directs energy and blood flow to required regions against stress, are impaired in the elderly. Stomach is very sensitive to stress and cortisol effects. Acute and chronic stress can produce different cortisol responses. The aim of this study was to evaluate the effect of acute and chronic stress on gastric inflammation markers in elderly female rats.
Materials and Methods: Sprague Dawley female rats (n = 18) aged 24-30 months were evaluated in the control, acute stress (60-minute restraint stress) and chronic stress (restraint stress for 14 day) groups. Following stress, serum corticosterone, TNF-α and IL-10 levels in gastric tissues were examined by ELISA method. Results were analyzed by One Way Anova and post-hoc Tukey test in SPSS 24 program. Pearson correlation analysis was used and p<0.05 was considered significant.
Results: Serum corticosterone levels increased in the acute stress group but did not change in the chronic stress group compared to controls. Gastric TNF-α levels increased both in the acute and chronic stress group compared to controls. There was a positive correlation between corticosterone and TNF-α in acute stress and control groups. IL-10 levels did not change in groups.
Conclusion: In elderly female rats, lower corticosterone response occurred with chronic stress. This may be related to insufficiency of glucocorticoid replacement ability of adrenal gland. Markers of acute inflammation did not increase further in chronic stress due to possible adaptation mechanisms of the stomach. Corticosterone caused changes in TNF-α levels, a proinflammatory cytokine in the stomach, during acute stress.

Keywords

elderly, stomach, stress

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