Apikal Periodontitisin Mikrobiyolojisi ve Tanı Yöntemleri

Year 2024, Volume: 10 Issue: 2, 65 - 71, 28.08.2024

Melisa Uslu , Sevinç Aktemur Türker

https://doi.org/10.21306/dishekimligi.1455438

Abstract

Apikal periododontitis (kök kanal enfeksiyonu) mikroorganizmaların ve metabolitik ürünlerinin kök kanalından periradiküler alana geçişi sonucu oluşan sert doku rezorpsiyonu ve periodontal ligamentin yıkımı ile karakterize inflamatuar ve enfeksiyöz bir hastalıktır. Bu derlemenin amacı kök kanal enfeksiyonlarını ve bu enfeksiyona sebep olan mikroorganizmaları ve tespit yöntemlerini mevcut literatür bilgisiyle gözden geçirmektir. Kök kanalında bulunan mikroorganizma çeşitliliği oldukça fazladır ve moleküler yöntemlerin gelişmesiyle birlikte kök kanal enfeksiyonlarına sebep olan mikroorganizmaların tespit edilebilirliği artmaktadır. Diş hekimlerinin kök kanal enfeksiyonlarına karşın etkili tedavi stratejileri geliştirmek için mikrobiyal flora hakkında bilgi sahibi olmalıdır.

Keywords

Kök kanal enfeksiyonları, Moleküler yöntemler, Yeni nesil dizileme

Ethical Statement

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Supporting Institution

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Project Number

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Thanks

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Microbiology of Apical Periodontitis and Diagnostic Methods

Abstract

Apical periododontitis (root canal infection) is an inflammatory and infectious disease characterized by hard tissue resorption and destruction of the periodontal ligament caused by the passage of microorganisms and their metabolites from the root canal to the periradicular area. The aim of this review is to look over root canal infections and the microorganisms that cause these infections and their detection methods with the current literature knowledge. The diversity of microorganisms found in the root canal is quite high and with the development of molecular methods, the detection of microorganisms causing root canal infections is increasing. Dentists should have knowledge about the microbial flora to develop effective treatment strategies against root canal infections.

Keywords

Root canal infections, Molecular techniques, New generation sequencing

Project Number

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References

• 1. Siqueira JF, Rôças IN. Microbiology of apical periodontitis. In:Dag Ørstavik editor. Essential Endodontology: Prevention and Treatment of Apical Periodontitis. John Wiley & Sons Ltd. 3 rd ed. 2019. 91– 142.
• 2. Aas JA, Griffen AL, Dardis SR, Lee AM, Olsen I, Dewhirst FE, Leys EJ, Paster BJ. Bacteria of Dental Caries in Primary and Permanent Teeth in Children and Young Adults. J Clin Microbiol. 2008; 46:1407.
• 3. Willoughby Dayton Miller. Zahnarzt. 1984;28:375- 376.
• 4. Nagaoka S, Miyazaki Y, Liu HJ, Iwamoto Y, Kitano M, Kawagoe M. Bacterial invasion into dentinal tubules of human vital and nonvital teeth. J Endod. 1995;21:70–3. 5. Rôças IN, Siqueira JF. Identification of bacteria enduring endodontic treatment procedures by a combined reverse transcriptase-polymerase chain reaction and reverse-capture checkerboard approach. J Endod. 2010;6:45–52.
• 6. Neelakantan P, Romero M, Vera J, Daood U, Khan AU, Yan A, Cheung GSP. Biofilms in EndodonticsCurrent Status and Future Directions. Int J Mol Sci. 2017;18:1748.
• 7. Egan MW, Spratt DA, Ng YL, Lam JM, Moles DR, Gulabivala K. Prevalence of yeasts in saliva and root canals of teeth associated with apical periodontitis. Int Endod J. 2002 ;35:321–29.
• 8. Siqueira JF, Rôças IN, Baumgartner JC, Xia T. Searching for Archaea in infections of endodontic origin. J Endod. 2005;31:719–22.
• 9. Glick M, Trope M, Bagasra O, Pliskin ME. Human immunodeficiency virus infection of fibroblasts of dental pulp in seropositive patients. Oral Surg Oral Med Oral Pathol. 1991;71:733–36.
• 10. Slots J, Sabeti M, Simon JH. Herpesviruses in periapical pathosis: An etiopathogenic relationship? Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003;96:327–31.
• 11. Pashley DH. Dentin-predentin complex and its permeability: physiologic overview. J Dent Res. 1985;64:613–20.
• 12. Pinheiro ET, Gomes BPFA, Ferraz CCR, Sousa ELR, Teixeira FB, Souza-Filho FJ. Microorganisms from canals of root-filled teeth with periapical lesions. Int Endod J. 2003;36:1–11.
• 13. TaniIshii N, Wang C Y, Tanner A, Stashenko P. Changes in root canal microbiota during the development of rat periapical lesions. Oral Microbiol Immunol.1994;9:129–35.
• 14. Fabricious L, Dahlen G, Öhman AE, Möller AJR. Predominant indigenous oral bacteria isolated from infected root canals after varied times of closure. Scand J Dent Res. 1982;90:134–44.
• 15. Sundqvist G, Figdor D. Life as an endodontic pathogen. Endod Topics. 2003;6:3–28.
• 16. Kolenbrander PE, Andersen RN, Blehert DS, Egland PG, Foster JS, Palmer RJ. Communication among Oral Bacteria. Microbiol Mol Biol Rev. 2002;66:486–505.
• 17. Saito D, De Toledo Leonardo R, Mazza Rodrigues JL, Tsai SM, Höfling JF, Gonçalves RB. Identification of bacteria in endodontic infections by sequence analysis of 16S rDNA clone libraries. J Med Microbiol. 2006;55:101–07.
• 18. Rôças IN, Siqueira JF, Aboim MCR, Rosado AS. Denaturing gradient gel electrophoresis analysis of bacterial communities associated with failed endodontic treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2004; 98:741–49.
• 19. Peciuliene V, Reynaud AH, Balciuniene I, Haapasalo M. Isolation of yeasts and enteric bacteria in root-filled teeth with chronic apical periodontitis. Int Endod J. 2001; 34:429–34.
• 20. Rôças IN, Siqueira JF, Santos KRN. Association of Enterococcus faecalis with different forms of periradicular diseases. J Endod. 2004;30:315–20.
• 21. Foschi F, Cavrini F, Montebugnoli L, Stashenko P, Sambri V, Prati C. Detection of bacteria in endodontic samples by polymerase chain reaction assays and association with defined clinical signs in Italian patients. Oral Microbiol Immunol. 2005;20:289-95.
• 22. Şen BH, Safavi KE, Spångberg LSW. Colonization of Candida albicans on cleaned human dental hard tissues. Arch Oral Biol. 1997;42:513–20.
• 23. Waltimo TMT, Ørstavik D, Sirén EK, Haapasalo MPP. In vitro susceptibility of Candida albicans to four disinfectants and their combinations. Int Endod J. 1999; 32:421–29.
• 24. Siqueira JF. Periapical Actinomycosis and infection with Propionibacterium Propionicum. Endod Topics. 2003;6:78–95.
• 25. Siqueira JF, Rôças IN. Exploiting molecular methods to explore endodontic infections: Part 1--current molecular technologies for microbiological diagnosis. J Endod. 2005; 31:411–23.
• 26. Paster BJ, Dewhirst FE. Molecular Microbial Diagnosis. Periodontol 2000;51:38.
• 27. Durmaz R. Uygulamalı Moleküler Mikrobiyoloji. 2. Baskı, Nobel Tıp Kitabevi, 2001. ss. 100-110.
• 28. Shin JM, Luo T, Lee KH, Guerreiro D, Botero TM, McDonald NJ, Rickard AH. Deciphering Endodontic Microbial Communities by Next-generation Sequencing. J Endod. 2018;44(7):1080-87.
• 29. Darcan, C., & Türkyılmaz, O. (2018). Yeni Nesil Dizileme Teknolojisine Genel Bakış. Bilecik Şeyh Edebali Üniversitesi Fen Bilimleri Dergisi 5(1), 41-49.
• Schuster SC. Next-generation sequencing transforms today’s biology. Nat Methods. 2008;5:16–8.
• 30. Ronaghi M, Nygren M, Lundeberg J, Nyrén P. Analyses of secondary structures in DNA by pyrosequencing. Anal Biochem. 1999; 267:65–71.
• 31. Margulies M, Egholm M, Altman WE, Attiya S, Bader JS, Bemben LA, et al. Genome sequencing in microfabricated high-density picolitre reactors. Nature. 2005; 437:376–80.
• 32. Shin JM, Luo T, Lee KH, Guerreiro D, Botero TM, McDonald NJ, Rickard AH. Deciphering Endodontic Microbial Communities by Nextgeneration Sequencing. J Endod. 2018; 44:1080–87.
• 33. Manoil D, Al-Manei K, Belibasakis GN. A Systematic Review of the Root Canal Microbiota Associated with Apical Periodontitis: Lessons from Next-Generation Sequencing. Proteomics Clin Appl. 2020; 14(3):e1900060.
• 34. Nardello LCL, Amado PPP, Franco DC, Cazares RXR, Nogales CG, Mayer MPA, Karygianni L, Thurnheer T, Pinheiro ET. Next-Generation Sequencing to Assess Potentially Active Bacteria in Endodontic Infections. J Endod. 2020;46:1105-12.
• 35. Kesim B, Ülger ST, Aslan G, Cudal H, Üstün Y, Küçük MÖ. Amplicon-based next-generation sequencing for comparative analysis of root canal microbiome of teeth with primary and persistent/secondary endodontic infections. Clin Oral Investig. 2023;27:995-1004.
• 36. Amaral RR, Love RM, Braga T, Souza Côrtes MI, Rachid CTCC, Rôças IN, Siqueira JF Jr. Impact of root canal preparation using two single-file systems on the intra-radicular microbiome of teeth with primary apical periodontitis. Clin Oral Investig. 2024;28:139.
• 37. Pérez-Carrasco V, Uroz-Torres D, Soriano M, Solana C, Ruiz-Linares M, Garcia-Salcedo JA, AriasMoliz MT. Microbiome in paired root apices and periapical lesions and its association with clinical signs in persistent apical periodontitis using nextgeneration sequencing. Int Endod J. 2023;56:622- 636.
• 38. Keskin C, Demiryürek EÖ, Onuk EE. Pyrosequencing Analysis of Cryogenically Ground Samples from Primary and Secondary/Persistent Endodontic Infections. J Endod. 2017;43:1309-16.