BİR MUHABBET KUŞUNDA ASPERGİLLUS ENFEKSİYONU

Yıl 2019, Sayı: 3, 70 - 75, 15.06.2019

Banur Buynukara , Timur Gülhan , Mine Aydın Kurç

Öz

Tüy dökülmesi ve kaşıntı şikâyeti ile Veteriner Fakültesi Mikrobiyoloji Anabilim Dalına canlı olarak getirilen 1 yaşında bir muhabbet kuşundan alınan tüy örnekleri mikolojik olarak incelendi. Bu amaçla tüy örneklerinden Sabouraud Dekstroz Agar (SDA) ve kloramfenikol içeren SDA besiyerlerine ekim yapıldı. İnkübasyon süresi sonunda üreyen küf kolonilerinin; koloni büyüklüğü ve rengi, yüzey görünümü, pigment oluşumu makroskobik olarak değerlendirildi. Kolonilerden Laktofenol pamuk mavisi (LFPM) ile pereparatlar hazırlanarak, mikroskopta konidiaforun uzunluğu, vesikülün şekli ve genişliği, konidianın şekli gibi özellikler açısından mikroskobik muayene yapıldı. Üreyen iki koloni makroskobik ve mikroskobik incelemeleri sonucu Aspergillus flavus (yeşil koloni) ve Aspergillus niger (siyah koloni) olarak tanımlandı.

Anahtar Kelimeler

Muhabbet kuşu, tüy, aspergillozis, mikroskopik muayene

Aspergillus Infection in a Budgerigar

Öz

In this study, feather samples taken from a one-years-old budgerigar with the complaint of hair loss and itching brought live to Department of Microbiology, Faculty of Veterinary Medicine were examined mycologically. For this purpose, the feather samples were plated on Sabouraud Dextrose Agar (SDA) and SDA medium containing chloramphenicol. Mildew colonies that grow at the end of the incubation period; colony size and color, surface appearance, pigment formation evaluated macroscopically. Smears prepared from colonies were stained with Lactophenol cotton blue (LPCB) and microscopic examination was performed in terms of the length of the conidiaphor, the shape and width of the vesicle, and the shape of the conidia. Macroscopic and microscopic examination of the two colonies revealed Aspergillus flavus (green colony) and Aspergillus niger (black colony).

Anahtar Kelimeler

Budgerigar, feather, aspergillosis, microscopic examination

Kaynakça

• Abrams, G.A., Paul-Murphy, J., Ramer, J.C. & Murphy, C.J. (2001). “Aspergillus blepharitis and dermatitis in a peregrine falcon-gyrfalcon hybrid (Falco peregrinus x Falco rusticolus”. J. Avian Med. Surg, 15, 114-120.
• Atasever, A. & Gümüşsoy, K.S. (2004). “Pathological, clinical and mycological findings in experimental aspergillosis infections of starlings”. J. Vet. Med.. A, Physiol. Pathol. Clin. Med, 51, 19-22.
• Barton, J.T., Daft, B.M., Read, D.H., Kinde, H. & Bickford, A.A. (1992). “Tracheal aspergillosis in 6 1/2-weekold chickens caused by Aspergillus flavus”. Avian Dis, 36, 1081-1085.
• Beckman, B.J., Howe, C.W., Trampel, D.W., DeBey, M.C., Richard, J.L. & Niyo, Y. (1994). “Aspergillus fumigatus keratitis with intraocular invasion in 15-dayold chicks”. Avian Dis., 38, 660-665.
• Beernaert, L.A., Pasmans, F., Van Waeyenberghe, L., Haesebrouck, F., & Martel, A. (2010). “Aspergillus infections in birds: A review”. Avian Pathol, 39, 325-331.
• Carrasco, L., Lima, J.S., Halfen, D.C., Salguero, F.J., Sanchez-Cordon, P. & Becker, G. (2001). “Systemic aspergillosis in an oiled Magallanic penguin (Spheniscus magellanicus)”. J. Vet. Med.. B, Infect. Dis. Vet. Public Health, 48, 551-554.
• Elad, D. (2018). “Therapy of non-dermatophytic mycoses in animals”. J Fungi, 4(120), 1-16, doi:10.3390/ jof4040120.
• Elad, D., & Segal, E. (2018). “Diagnostic aspects of veterinary and human aspergillosis”. Front Microbiol, 9:1303. doi: 10.3389/fmicb.2018.01303.
• Femenia, F., Fontaine, J., Lair-Fulleringer, S., Berkova, N., Huet, D., Towanou, N., Rakotovao, F., Granet, O.I., Le Loch, G., Arné, P., Guillot, J. (2007). “Clinical, mycological and pathological findings in turkeys experimentally infected by Aspergillus fumigatus”. Avian Pathol, 36 (3), 213-219.75
• Fischer, D., & Lierz, M. (2015). “Diagnostic procedures and available techniques for the diagnosis of aspergillosis in birds”. J. Exot. Pet Med, 24, 283-295.
• Hoppes, S., Gurfield, N., Flammer, K., Colitz, C. & Fisher, P. (2000). “Mycotic keratitis in a blue-fronted Amazon parrot (Amazona aestiva)”. J. Avian Med. Surg, 14, 185-189.
• Girma, G., Abebaw, M., Zemene, M., Mamuye, Y., & Getaneh, G. (2016). “A review on aspergillosis in poultry”. J. Vet. Sci. Technol. 7:382. doi: 10.4172/2157- 7579.1000382
• Jung, K., Kim, Y., Lee, H. & Kim, J.T. (2009).“Aspergillus fumigates infection in two wild Eurasian black vultures (Aegypius monachus Linnaeus) with carbofuran insecticide poisoning: a case report”. Vet. J, 179, 307-312.
• Kelly, T.R., Vennen, K.M., Duncan, R. & Sleeman, J.M. (2004). “Lymphoproliferative disorder in a great horned owl (Bubo virginianus)”. J. Avian Med. Surg, 18, 263-268.
• Khosravi, A.R., Shokri, H., Ziglari, T., Naeini, A.R., Mousavi, Z. & Hashemi, H. (2008). “Outbreak of severe disseminated aspergillosis in a flock of ostrich (Struthio camelus)”. Mycoses, 51, 557-559.
• Klich, M.A. (2002). “Identification of Common Aspergillus Species”. Central Bureauvoor Schimmelcultures, Utrecht, The Netherlands, 116 p.
• König, H. E., Navarro, M., Zengerling, G., & Korbel, R. (2016). “Respiratory system (apparatus respiratorius), in Avian Anatomy Textbook and Colour Atlas”, 2nd Edn, eds H. E.König, R. Korbel, and H. G. Liebich (Sheffeld: 5M Publishing Ltd), 118–130.
• Lamoth, F. (2016). “Aspergillus fumigatus-related species in clinical practice”. Front Microbiol, 7:683. doi: 10.3389/fmicb.2016.00683.
• Nardoni, S., Ceccherelli, R., Rossi, G. & Mancianti, F. (2006). “Aspergillosis in Larus cachinnans micaellis: survey of eight cases”. Mycopathol, 161, 317-321.
• Neuman, N. (2016). “Aspergillosis in domesticated birds”. J. Comp. Pathol. 155, 102-104.
• Sabino, R., Burco, J., Valente, J., Veríssimo, C., Clemons, K.V., Stevens, D.A., & Tell, L.A. (2019). “Molecular identification of clinical and environmental avian Aspergillus isolates”. Arch Microbiol, 201, 253-257.
• Samanta, I., & Bandyopadhyay, S. (2017). “Pet Bird Diseases and Care. Singapore: Springer”. doi: 10.1007/978-981-10-3674-3.
• Savelieff, M.G., Pappalardo, L., & Azmanis, P. (2018). “The current status of avian aspergillosis diagnoses: Veterinary practice to novel research avenues”. Vet Clin Pathol, 47, 342-362.
• Suedmeyer, W.K., Bermudez, A.J. & Fales, W.H. (2002). “Treatment of epidermal cysts associated with Aspergillus fumigatus and alternaria species in a silky bantam chicken.” J. Avian Med. Surg, 16, 133-137.
• Talbot, J.J., Thompson, P., Vogelnest, L., & Barrs, V.R. (2018). “Identification of pathogenic Aspergillus isolates from captive birds in Australia”. Med Mycol, 56 (8), 1038-1041.
• Tell, L.A. (2005). “Aspergillosis in mammals and birds: impact on veterinary medicine”. Medical Mycology Supplement, 1, S7_S73.
• Verstappen, F.A.L.M. & Dorrestein, G.M. (2005).“Aspergillosis in Amazon parrots after corticosteroid therapy for smoke-inhalation injury”. J. Avian Med. Surg, 19, 138-141.
• Ziolkowska, G., Tokarzewski, S., & Nowakiewicz, A. (2014). “Drug resistance of Aspergillus fumigatus strains isolated from flocks of domestic geese in Poland”. Poult Sci, 93, 1106-1112.