MUTATION ANALYSIS OF THE PROTO-ONCOGENES Ki-RAS AND C-MYC IN THE SOFT TISSUE TUMORS OF THE RATS THAT WERE FORMED BY 3-METHYLCHOLANTHRENE IN VIVO

Abstract

Polisiklik aromatik hidrokarbonlar en potent karsinojenler olarak bilinmektedir. 3-Metilkolantren PAH’ların

içinde yer alan karsinojenik bir ajandır. 3-Metilkolantren’in memeli sistemlerinde deneysel olarak toksik ve

karsinojenik etkileri gösterilmiştir. Bu bileşiklere çevrede zift, kurum, sıvı ve katı yakıtlar nedeniyle havada,

ekzos gazında, sigara dumanında, dumanlanmış yiyeceklerde sıklıkla rastlanmaktadır. Bu çalışmanın amacı 3-

Metilkolantren’ in 40 mg/kg’lık bir dozda kronik olarak ratlara verilmesiyle oluşan tümörlerde Ki-ras ekson 1, 2

ve C-myc ekson 2 genlerinde herhangi bir mutasyona neden olup olmadığını tespit etmektir. Ratlar son

enjeksiyondan 26 hafta sonra servikal dislokasyon ile öldürüldü ve oluşan yumuşak doku tümörleri çıkarıldı.

Dokular belirtilen genler için PCR tabanlı SSCP analiziyle incelendi. Sonuç olarak Ki-ras ekson 1, 2 ve C-myc

ekson 2 genlerinde hiçbir nokta mutasyonu görülmedi.

Keywords

• Ki-ras geni,C-myc geni,3-Metilkolantren,rat

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