THE VARIATIONS IN FATTY ACIDS COMPOSITION OF OVARIES AND TESTIS DURING GONAD MATURATION OF PIKEPERCH (Sander iucioperca)

Abstract

Abstract The aim of this study was to determine variations in total lipid, total fatty acid and fatty acid composition. of ovaries and testis with respect to the gonad maturation of pikeperch in Egirdir lake. Gonads were taken from fishes in November (a period of initiation of gonad maturation) and in March (a period of completion of gonad maturation). The increases in total lipid content were insignificant for testis (P>O.05) but significant for ovaries (P<O.05) In November and March. Total fatty acids constituted more than half of total lipid in both ovaries and testis. Palmitic (16:0), oleic (18:1), docosahexaenoic(22:6n-3), eicosapentaenoic(20:5n-3) and arachidonic(20:4n-6) acids were the most abundant fatty acids present in gonads. The ratio of polyunsaturated fatty acids (PUFA) were remarkably high in March compared with that of PUFA in November. It was concluded that a significant amount of PUFA is required for gonad maturation of pikeperch.

Keywords

• Fatty Acid,Gonad,Pike perch

GONAD OLGUNLASMASI ESNASINDA SUDAK (Sander lucioperca) BALIGININ OVARYUM VE TESTisLERiNiN YAG ASiDi BiLESiMiNDEKi DEGiSiMLER

Abstract

Bu cahsmanm amaci: Egirdir Golu'nde yasayan sudaklann gonad gelisirni ile ilgili olarak ovaryum ve testislerinin toplam lipid, toplam yag asidi ve yag asidi bilesimlerindeki degisimlerin incelenmesidir. Gonadlar, gelisirnin basladrgi Kasun ve gelisirrun tarnamlandrgi Mart aylannda cahsrlrrusnr, Kasim ve Mart aylannda toplam lipid icerigindeki arns testisler icin onernsiz (P>o.05), ovaryumlar icin onernli (P<O.05) bulunrnustur. Hem ovaryum hem de testislerde toplam lipid iceriginin yansmdan fazlasim toplam yag asitleri olusturmustur. Gonadlarda palmitik (16:0), oleik (18: I), dokosaheksaenoik (22:6n-3), okosapentaenoik (20:5n-3) ve arasidonik (20:4n-6) asitler en cok bulunan yag asitleridir. Coklu doyrnarrus yag asitleri (PUFA) oram Mart aymda Kasun ayma gore onernli de recede yuksek bulunrnustur. Sudaklann gonad olgunlastirmasr icin PUFA lere onernli derecede ihtiyac duydugu sonucuna vanlrrusnr.

Keywords

• Gonad,Sudak,Yag Asidi

References

1. [1] Ackman, R.O., 1967. "Characteristics of the Fatty Acid Composition and Biochemistry of Some Freshwater Fish Oils and Lipids in Comparison with Marine Oils and Lipids" Compo Biochem, Physiol., 22, 907-922.
2. [2] Agren, J., Muje, P., Hanninen, 0., Herranen, J., Pentula, I., 1987. "Seasonal Variations of Lipid Fatty Acids of Boreal Freshwater Fish Species" Compo Biochem. Physiol., 88, 905-909.
3. [3] Ahlgren, G., 1996. "Fatty Acid Content of Some Freshwater Fish in Lakes of Different Trophic Levels" Ecol. Freshwater Fish, 5 (1), 15-27.
4. [4] Andrade, A.P., 1995. "Omega3 Fatty Acids in Freshwater Fish From South Brazil" Jour. of the American Oil Chemists, 72 (10), 1207- 1210.
5. [5] Becer, Z.A., 1995. Egirdir ootu lucioperca Lin., 1758) Populasyonunun Uzerine Bir Arastirma. S.D.U. Fen . Bil. Muhendisligi A.B.D., Yuksek Lisans Tezi, Sudak ( Stizostedion Yapisi ve Gelismesi Enst. Su Urunleri 63s. Egirdir - Isparta.
6. [6] Bligh, E.G., and Dyer, J.M., 1959. "A Rapid Medhod of Total Lipid Extraction and Purification" Can. Jour. Biochem. and Physiol., 37, 911-917.
7. [7] Deng, J.C., Orthefer, F.T., Dennison, R.L., Watson, M., 1976. "Lipids and Fatty Acids in Mullet (Mugil cephalus): Seasonal and Locational Variations" Jour. of Food Science, 4, 1479-1483.
8. [8] Duncan, D.B., 1955. Multiple Range and Multiple F-Tests. Biometrics. 11, 1- 41.

9. [9] Folch, J., Lees, M., Stanley, G.H., 1956. "A Simple Method for the Isolation and Purification of Total Lipids From Animal Tissues" The Jour. BioI. Chern., 2261, 497-509.
10. [10] Huss, H., 1988. "Fresh Fish Quality and Quality Changes" Ministery of Fisheries Technical University Press, Copanhagen, Denmark.
11. [11] Kaitaranta, J.K., Acman, R.G., 1981. Total Lipids and Lipid Classes of Fish Roe. Compo Biochem. Physiol., 69, 725-729.
12. [12] Kiessling, A., Johansson, L., Storebakken, T., 1989. Effects of Reduced Feed Ration Levels on Fat Content and Fatty Acid Composition in White and Red Muscle From Rainbow Trout. Aquaculture, 79, 169-175.
13. [13] Love, M., 1970. The Chemical Biology of Fishes. Press, 547p. New York. Academic
14. [14] Medford, B.A., Mackay, W.C., 1978. "Protein and Lipid Content of Gonads, Liver And Muscle of Northern Pike (Esox lucius) in Relation to Gonad Growth" Jour. Fisheries Research Board of Canada, 35, 213-219.
15. [15] Metin, K., 1992. Topardic Deresindeki (Kangal - Sivas) Cyprinion macrostomus Hekel, 1948 (Osteichthyes: Cyprinidae)'lann Gonadal Total Lipid, Total Yag Asidi ve Glikojen lceriginin Mevsimsel Degisirni. Cumhuriyet Univ. Fen Bil. Enst. Biyoloji AB.D., Ytiksek Lisans Tezi, 65s, Sivas.
16. [16] Moss, C.W., Lambert, M.A Merwin, W.H., 1974. "Comparision of Rapid Medhods for Analysis of Bacterial Fatty Acids" Applied Microbiology, 28, 80-85.
17. [17] Soivio, A, Nrerrusto, M., Backsrom, M., 1989. Fatty Acid Composition of Coregon us muksun Pallas: Changes During Incubation, Hatching, Feeding and Starvation. Aquaculture, 79, 163-168.
18. [18] Uysal, K., 2000. Egirdir Golu Sudak ( Stizostedion lucioperca Lin., 1758) Baliklannm Total Lipid, Total Yag Asidi ve Yag Asidi Bilesiminin Mevsimsel incelenmesi. Fen Bil. Enst. Su Urunleri femel Bilimleri A.B.D., Doktora Tezi, 66s. Egirdir - . Isparta.
19. [20] Wang, Y.J., Miller, L.A, Perren, M., Addis, P.B., 1990. Omega3 Fatty Acids In Lake Superior Fish. Jour. of Food Science, 55, 72-73.