EVALUATION OF TOTAL ANTIOXIDANT AMOUNT AND OXIDATION STABILITY IN EDIBLE OILS

Abstract

The DPPH method is one of the most commonly used methods of measuring the total antioxidant level of many food products. The Schaal furnace test, the Active Oxygen Method (AOM) and the Rancimat analysis method are methods used to determine the oxidation stability of oils. In this study, the oxidation stability of refined sunflower oil, refined corn oil and extra virgin olive oil was analyzed with ransimat 743 total antioxidant value was determined by DPPH method. The relationship between total antioxidant amount and oxidation stability has been determined. According to the results of the study, the highest antioxidant content and oxidation stability has determined as oil extra virgin olive oil and the lowest refined sunflower oil. Although the oils are different, antioxidant oils have been shown to have a positive effect on oxidation stability. The activity of the DPPH solution in methyl alcohol has maintained at room temperature and in the dark has also evaluated and the activity decreased 3.45% at 15 minutes; decreased 6.21% at the 30th minute; decreased 17.53%. at the 45th minute.

Keywords

• DPPH,EC50,Anti radical power,UV Spectrophotometry,Antioxidant

YEMEKLİK YAĞLARDA TOPLAM ANTİOKSİDAN MİKTARININ VE OKSİDASYON KARARLILIĞININ DEĞERLENDİRİLMESİ

Abstract

DPPH metodu birçok gıda ürününün toplam antioksidan seviyesinin ölçülmesinde en çok kullanılan metotlardan biridir. Schaal fırın testi, Aktif Oksijen Metodu (AOM) ve Ransimat analiz metodu yağların oksidasyon kararlılığının tespitinde kullanılan metotlardır. Bu çalışmada rafine ayçiçek yağı, rafine mısırözü yağı ve sızma zeytinyağının oksidasyon kararlılıkları ransimat 743 ile toplam antioksidan tayini DPPH metodu yardımıyla tespit edilmiş toplam antioksidan miktarıyla oksidasyon kararlılığı arasındaki ilişki tespit edilmeye çalışılmıştır. Çalışmanın sonucuna göre antioksidan içeriği ve oksidasyon kararlılığı en yüksek yağ sızma zeytinyağı, en düşük Rafine ayçiçek yağı olarak tespit edilmiştir. Yağların farklı olmasına rağmen antioksidan içeriğin yağların oksidasyon kararlılıklarına olumlu etki yaptığı gözlenmiştir. Oda sıcaklığında ve karanlıkta muhafaza edilen metil alkol içinde DPPH çözeltisinin etkinliği de değerlendirilmiş olup 15. Dakikada etkinliği %3,45 azalmış; 30. Dakikada %6,21 azalmış; 45. Dakikada ise %17,53 azalmıştır.

Keywords

• DPPH,EC50,Anti radikal güç,UV Spektrofotometri,Antioksidan

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