EFFECTS OF JUGLONE APPLIED IN PREGERMINATIVE STAGE ON GROWTH OF CUCUMBER SEEDLINGS WITH RESPECT TO PHYSIOLOGICAL AND ANATOMICAL PARAMETERS

Year 2006, Issue: 010, 25 - 32, 15.05.2006

İ. Terzi İ. Kocaçalışkan O. Benlioğlu

Abstract

Effects of juglone on seedling growth of cucumber (Cucumis sativus cv. Beith Alpha) with respect to physiological and anatomical parameters were investigated. Growth parameters (seedling elongation, fresh and dry weights) were reduced by 1 mM juglone. At the same time, juglone decreased xylem vessel and bundle radius of stem, and number and length of stomata of the cotyledons. But increases in thickness of the cotyledon mesophyll were found. In addition, juglone caused to decrease in protein and chlorophyll content and catecholase and tyrosinase activities. Growth inhibitory effect of juglone was discussed to be related to reduce in anatomic structures such as xylem vessel and stomata rather than the chlorophyll content and the enzyme activities.

Keywords

Anatomy, Cucumber, Enzyme Activity, Juglone, Seedling Growth

ÇİMLENME ÖNCESİ UYGULANAN JUGLONUN HIYAR FİDELERİNDE BÜYÜME İLE İLGİLİ FİZYOLOJİK VE ANATOMİK PARAMETRELER ÜZERİNE ETKİLERİ

Abstract

Bu çalışmada çimlenme öncesi uygulanan juglonun fizyolojik ve anatomik parametrelerle ilgili olarak hıyar (Cucumis sativus cv. Beith Alpha)’ın fide büyümesi üzerine etkileri araştırıldı. Büyüme parametreleri (fide büyümesi, taze ve kuru ağırlık) 1mM juglon tarafından azaltıldı. Aynı zamanda juglon anatomik yapılardan gövdenin trake ve demet çapı ile kotiledonlardaki stoma sayısını ve stoma uzunluğunu azalttı. Buna mukabil kotiledon mezofilinde kalınlaşmalar belirlendi. Ayrıca juglon protein ve klorofil miktarı ile katekolaz ve tirozinaz enzim aktivitelerinde azalmaya sebep oldu. Juglonun büyüme engelleyici etkisinin klorofil miktarı ile enzim aktivitelerinden ziyade trake ve stoma gibi anatomik yapılardaki indirgenme ile ilgili olabileceği tartışıldı.

Keywords

Fide Büyümesi, Hıyar, Juglon, Katekolaz, Tirozinaz

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