Kanser Araştırmalarında Laboratuvar Hayvanı Modellerinde Geleneksel Yaklaşımlar ve Yenilikçi Stratejiler: Kapsamlı Bir İnceleme

Year 2024, , 36 - 46, 30.06.2024

Mümin Alper Erdoğan

https://doi.org/10.18678/dtfd.1496879

Abstract

Kanser, tıbbi araştırmalarda önemli bir zorluk olarak kalmaya devam etmektedir ve karmaşıklığını anlamak ve etkili tedaviler geliştirmek için çeşitli ve karmaşık modellere ihtiyaç duyulmaktadır. Bu derlemenin amacı, deneysel kanser modellerinin evrimini ve faydasını incelemek ve temel araştırma ile klinik uygulama arasındaki boşluğu kapatmada önemli rol oynadıklarını vurgulamaktır. Tümör büyümesini ve ilaç yanıtını bir canlı organizmada çalışmak için doğrudan bir yol sağlayan ksenograftların geleneksel kullanımından, insan kanserinin genetik ve fenotipik özelliklerini kopyalayan genetiği değiştirilmiş fare modellerinin (genetically engineered mouse models, GEMMs) yenilikçi yaklaşımlarına kadar, her model kanser biyolojisine benzersiz bir bakış sunmaktadır. Son dönemdeki ilerlemeler, tümörün mikroçevresini yakından taklit eden üç boyutlu bir perspektif sunan organoid modellerini ve hastalık ilerlemesini ve tedavi sonuçlarını tahmin etmek için hastaya özgü verileri kullanan hesaplama modellerini tanıtmıştır. Bu modeller, kanserin moleküler etkenlerinin anlaşılmasına yardımcı olmakta, hedefe yönelik tedavilerin geliştirilmesini kolaylaştırmakta ve onkolojide kişiselleştirilmiş tıbbın önemini vurgulamaktadır. Bu deneysel modellerin çeşitliliği ve potansiyeline rağmen, tümörün karmaşıklığının kopyalanması ve bağışıklık sistemi etkileşimlerinin entegrasyonu gibi zorluklar devam etmektedir. Gelecekteki araştırmalar, tahmin doğruluklarının artırılmasına ve güçlü yanlarının birleştirilmesine odaklanarak kanser biyolojisi ve tedavisine bütünsel bir bakış açısı sunmak için bu modellerin iyileştirilmesine yöneliktir.

Keywords

Kanser deneysel araştırma, ksenograft, genetiği değiştirilmiş fare modelleri, organoid model, hesaplamalı modeller, kişiselleştirilmiş tıp, tümör mikroçevre

Traditional Approaches and Innovative Strategies in Laboratory Animal Models for Cancer Research: A Comprehensive Review

Abstract

Cancer remains one of the foremost challenges in medical research, necessitating diverse and sophisticated models to understand its complexity and develop effective treatments. This review explores the evolution and utility of experimental cancer models, highlighting their pivotal role in bridging the gap between basic research and clinical application. From the traditional use of xenografts, which provide a direct avenue for studying tumor growth and drug response in a living organism, to the innovative approaches of genetically engineered mouse models (GEMMs) that replicate human cancer's genetic and phenotypic traits, each model offers unique insights into cancer biology. Recent advances have introduced organoid models, offering a three-dimensional perspective that closely mimics the tumor's microenvironment, and computational models, which leverage patient-specific data to predict disease progression and treatment outcomes. These models enhance our understanding of cancer's molecular drivers, facilitate the development of targeted therapies, and underscore the importance of personalized medicine in oncology. Despite the diversity and potential of these experimental models, challenges remain, including the replication of the tumor's complexity and the integration of immune system interactions. Future research is directed toward refining these models, improving their predictive accuracy, and combining their strengths to offer a holistic view of cancer biology and treatment.

Keywords

Cancer experimental research, xenograft, genetically engineered mouse models, organoid model, computational models, personalized medicine, tumor microenvironment

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