Evaluation of the Effects of Acute Cisplatin Administration on Testicular and Ovarian Tissue in Rats

Abstract

Aim: Cisplatin, one of the effective chemotherapeutics in cancer treatment, has the potential to affect the testis and ovary, leading to permanent or temporary infertility. This study aimed to determine the acute effects of cisplatin on testis and ovary histology and to investigate whether it induces any changes in immunohistochemical cyclooxygenase-2 (COX2), nuclear factor kappa B p65 (NFκB-p65), and heat-shock protein 70 (HSP70) levels. Material and Methods: The study was planned as four groups: male control, male cisplatin, female control, and female cisplatin. The cisplatin group rats were adminestered with 7 mg/kg cisplatin, and all rats were sacrificed 24 hours later. Hematoxylin-eosin and Masson's trichrome stains were applied to testis and ovary tissues to examine their histopathological structure, and an immunohistochemistry staining protocol was applied to determine immunostaining intensity of COX2, NFκB-p65, and HSP70. Results: In the cisplatin group, a decrease in seminiferous tubule epithelium, an elevation in fibrotic response in the interstitial area, and a notable reduction in Johnson testicular biopsy score (p<0.001) were seen in the testis. In the ovary, atretic follicles (p=0.006) and luteal structures within the cortex, as well as vascular congestion (p=0.001), edema (p=0.001), and fibrotic areas within the medulla, were evident. These alterations resulted in a statistically significant increase in ovarian histoscores, except for leukocyte infiltration (p=0.322). In both tissues, cisplatin significantly increased the immunostaining intensity of COX2, NFκB-p65, and HSP70 compared to the control group. Conclusion: Acute cisplatin administration can induce tissue damage and pro-inflammatory response in the testis and ovary.

Keywords

• cisplatin
• testis
• ovary
• cyclooxygenase-2
• NF-kappa B p65
• heat-shock protein 70

Sıçanlarda Akut Sisplatin Uygulamasının Testis ve Yumurtalık Dokusundaki Etkilerinin Değerlendirilmesi

Abstract

Amaç: Kanser tedavisinde etkili kemoterapötiklerden biri olan sisplatin, testis ve overi etkileyerek kalıcı veya geçici infertiliteye yol açma potansiyeline sahiptir. Bu çalışma, sisplatinin testis ve over histolojisi üzerindeki akut etkilerini belirlemeyi ve immünohistokimyasal siklooksijenaz-2 (COX2), nükleer faktör kappa B p65 (NFκB-p65) ve ısı-şok protein 70 (HSP70) düzeylerinde herhangi bir değişikliğe neden olup olmadığını araştırmayı amaçlamıştır. Gereç ve Yöntemler: Çalışma dört grup olarak planlandı: erkek kontrol, erkek sisplatin, dişi kontrol ve dişi sisplatin. Sisplatin grubu sıçanlara 7 mg/kg sisplatin uygulandı ve tüm sıçanlar 24 saat sonra sakrifiye edildi. Testis ve over dokularına histopatolojik yapılarını incelemek amacıyla hematoksilen-eozin ve Masson trikrom boyaları uygulandı ve COX2, NFκB-p65 ve HSP70'in immün boyama yoğunluğunu belirlemek amacıyla immünohistokimya boyama protokolü uygulandı. Bulgular: Sisplatin grubunda, testiste seminifer tübül epitelinde azalma, interstisyel alanda fibrotik yanıtta artış ve Johnson testiküler biyopsi skorunda kayda değer bir azalma (p<0,001) görüldü. Overde, kortekste atretik foliküller (p=0,006) ve luteal yapılar yanı sıra medullada vasküler konjesyon (p=0,001), ödem (p=0,001) ve fibrotik alanlar belirgindi. Bu değişiklikler, lökosit infiltrasyonu (p=0,322) dışında, over histoskorlamasında istatistiksel olarak anlamlı bir artışla sonuçlandı. Her iki dokuda da, sisplatin, kontrol grubuna kıyasla COX2, NFκB-p65 ve HSP70 immün boyanma yoğunluğunu önemli ölçüde artırdı. artışa neden oldu. Sonuç: Akut sisplatin uygulaması, testiste ve overde doku hasarına ve proinflamatuar yanıta yol açabilir.

Keywords

• sisplatin
• testis
• over
• siklooksijenaz-2
• NF-kappa B p65
• ısı-şok protein 70

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