İlk ve Orta Adolesan Dönemdeki Erkek Sıçanlara Uygulanan 900-Megahertz Elektromanyetik Alanın Dalak Üzerine Etkileri: Biyokimyasal ve Histopatolojik Çalışma

Öz

Amaç: Adolesanlar cep telefonlarının yoğun kullanımından dolayı risk altındadır. Bu çalışmanın amacı ilk ve orta adolesan dönemlerde maruz kalınan 900-Megahertz elektromanyetik alanın, geç adolesan dönemde dalak üzerindeki histopatolojik ve biyokimyasal etkilerini araştırmaktır.

Gereç ve Yöntemler: Bu çalışmada 24 adet 21-günlük Sprague Dawley tipi adolesan erkek sıçan, kontrol (n=8), sham (n=8) ve elektromanyetik alan (n=8) gruplarına ayrıldı. Kontrol grubu sıçanlara herhangi bir uygulama yapılmadı. Elektromanyetik alan grubuna ayrılan sıçanlar, elektromanyetik alan kafesi içerisine alındı ve 900-Megahertz elektromanyetik alana (25 gün boyunca her gün 1 saat) maruz bırakıldı. Sham grubu sıçanlar ise elektromanyetik alan kafesine alındı fakat elektromanyetik alana maruz bırakılmadı. Uygulamaların bitiminde tüm hayvanlar servikal dislokasyon yöntemiyle sakrifiye edilerek dalakları çıkarıldı. Histolojik işlemlerden sonra, dokulardan kesitler alındı ve hematoksilen-eozin ve periyodik asit Schiff tekniğiyle ile boyandı. Dalak dokularında histopatolojik değerlendirme yapıldı. Biyokimyasal analizler ile oksidatif stres parametrelerinden lipit peroksidasyonu, süperoksit dismutaz, glutatyon ve katalaz düzeyleri incelendi.

Bulgular: Histopatolojik değerlendirmede elektromanyetik alan grubu adolesan sıçanların dalak dokularında megakaryosit hücreler, genişlemiş beyaz pulpalar ve dilate sinüzoidaller izlendi. Biyokimyasal analiz sonuçlarına göre glutatyon ve lipit peroksidasyonu değerlerinin arttığı, ancak süperoksit dismutaz ve katalaz değerlerinin azaldığı tespit edildi.

Sonuç: Adolesan dönemde uygulanan 900-Megahertz elektromanyetik alanın erkek sıçanların dalak dokusu üzerinde morfolojik yapıda değişiklikler meydana getirdiği ve oksidatif strese neden olduğu söylenebilir.

Anahtar Kelimeler

• adolesan,dalak,elektromanyetik alan,oksidatif stres

The Effect of 900-Megahertz Electromagnetic Field Exposure in the First and Middle Adolescent Period on the Spleen in Male Rats: A Biochemical and Histopathological Study

Abstract

Aim: Adolescents are at risk due to the intensive use of mobile phones. The aim of this study was to investigate the histopathological and biochemical effects of 900-Megahertz electromagnetic field on spleen in late adolescent period, exposed during periods of early and mid-adolescence.

Material and Methods: In this study, 24 Sprague Dawley 21-day-old male rats were divided into control (n=8), sham (n=8) and electromagnetic field groups (n=8). Control group rats were not subjected to any application. Electromagnetic field group rats were taken into the electromagnetic field cage and were exposed to 900-Megahertz electromagnetic field (1 hour per day for 25 days). Sham group rats were taken into the electromagnetic field cages but were not exposed to electromagnetic field. At the end of the treatment, all animals were sacrificed by cervical dislocation method and the spleens were removed. After histological procedures, tissue sections were taken and stained with hematoxylin-eosin and periodic acid Schiff. Histopathological evaluation was performed on the spleen tissues. Oxidative stress parameters including lipid peroxidation, superoxide dismutase, glutathione and catalase levels were investigated via biochemical analysis.

Results: Histopathological evaluation revealed megakaryocyte cells, enlarged white pulps and dilated sinusoids in spleen tissues of adolescent rats in electromagnetic field group. According to biochemical analysis results, it was determined that glutathione and lipid peroxidation values were increased, but superoxide dismutase and catalase values were decreased.

Conclusion: It can be said that the 900-Megahertz electromagnetic field applied in adolescent period caused morphological changes on spleen tissue and caused oxidative stress in male rats.

Keywords

• adolescent,spleen,electromagnetic field,oxidative stress

References

1. Aydin B. Effects of thiacloprid, deltamethrin and their combination on oxidative stress in lymphoid organs, polymorphonuclear leukocytes and plasma of rats. Pestic Biochem Phys. 2011;100(2):165-71.
2. Hancı H, Türedi S, Topal Z, Mercantepe T, Bozkurt I, Kaya H, et al. Can prenatal exposure to a 900 MHz electromagnetic field affect the morphology of the spleen and thymus, and alter biomarkers of oxidative damage in 21-day-old male rats? Biotech Histochem. 2015;90(7):535-43.
3. Li BL, Li W, Bi JQ, Zhao JG, Qu ZW, Lin C, et al. Effect of long-term pulsed electromagnetic field exposure on hepatic and immunologic functions of rats. Wien Klin Wochenschr. 2015;127(23-24):959-62.
4. Tirelli E, Laviola G, Adriani W. Ontogenesis of behavioral sensitization and conditioned place preference induced by psychostimulants in laboratory rodents. Neurosci Biobehav Rev. 2003;27(1-2):163-78.
5. Ulubay M, Yahyazadeh A, Deniz ÖG, Kıvrak EG, Altunkaynak BZ, Erdem G, et al. Effects of prenatal 900 MHz electromagnetic field exposures on the histology of rat kidney. Int J Radiat Biol. 2015;91(1):35-41.
6. Odacı E. Hancı H, İkinci A, Sönmez OF, Aslan A, Şahin A, et al. Maternal exposure to a continuous 900 MHz electromagnetic field provokes neuronal loss and pathological changes in cerebellum of 32-day-old female rat offspring. J Chem Neuroanat. 2016;75(Pt B):105-10.
7. İkinci A, Mercantepe T, Unal D, Erol HS, Şahin A, Aslan A, et al. Morphological and antioxidant impairments in the spinal cord of male offspring rats following exposure to a continuous 900-MHz electromagnetic field during early and mid-adolescence. J Chem Neuroanat. 2016;75(Pt B):99-104.
8. Aslan A, İkinci A, Baş O, Sönmez OF, Kaya H, Odacı E. Long-term exposure to a continuous 900 MHz electromagnetic field disrupts cerebellar morphology in young adult male rats. Biotech Histochem. 2017;92(5):324-30.

9. Durusoy R, Hassoy H, Özkurt A, Karababa AO. Mobile phone use, school electromagnetic field levels and related symptoms: a cross-sectional survey among 2150 high school students in Izmir. Environ Health. 2017;16(1):51.
10. Kerimoğlu G, Güney C, Ersöz Ş, Odacı E. A histopathological and biochemical evaluation of oxidative injury in the sciatic nerves of malerats exposed to a continuous 900-megahertz electromagnetic field throughout all periods of adolescence. J Chem Neuroanat. 2018;91:1-7.
11. Kim SH, Lee HJ, Choi SY, Gimm YM, Pack JK, Choi HD, et al. Toxicity bioassay in Sprague-Dawley rats exposed to 20 kHz triangular magnetic field for 90 days. Bioelectromagnetics. 2006;27(2):105-11.
12. Keleş Aİ, Yıldırım M, Gedikli Ö, Çolakoğlu S, Kaya H, Baş O, et al. The effects of a continuous 1-h a day 900-MHz electromagnetic field applied throughout early and mid-adolescence on hippocampus morphology and learning behavior in late adolescent male rats. J Chem Neuroanat. 2018;94:46-53.
13. Keleş Aİ, Nyengaard JR, Odacı E. Changes in pyramidal and granular neuron numbers in the rat hippocampus 7 days after exposure to a continuous 900-MHz electromagnetic field during early and mid-adolescence. J Chem Neuroanat. 2019;101:101681.
14. Arendash GW, Mori T, Dorsey M, Gonzalez R, Tajiri N, Borlongan C. Electromagnetic treatment to old Alzheimer's mice reverses β-amyloid deposition, modifies cerebral blood flow, and provides selected cognitive benefit. PLoS One. 2012;7(4):e35751.
15. Arendash GW, Sanchez-Ramos J, Mori T, Mamcarz M, Lin X, Runfeldt M, et al. Electromagnetic field treatment protects against and reverses cognitive impairment in Alzheimer’s disease mice. J Alzheimers Dis. 2010;19(1):191-210.
16. Karimi SA, Salehi I, Shykhi T, Zare S, Komaki A. Effects of exposure to extremely low-frequency electromagnetic fields on spatial and passive avoidance learning and memory, anxiety-like behavior and oxidative stress in male rats. Behav Brain Res. 2019;359:630-8.
17. İkinci A, Odacı E, Yıldırım M, Kaya H, Akça M, Hancı H, et al. The effects of prenatal exposure to a 900 Megahertz electromagnetic field on hippocampus morphology and learning behavior in rat pups. Neuroquantology.2013;11(4):582-90.
18. Baş O, Sönmez FO, Aslan A, İkinci A, Hancı H, Yıldırım M, et al. Pyramidal cell loss in the cornu ammonis of 32-day-old female rats following exposure to a 900 Megahertz electromagnetic field during prenatal days 13-21. Neuroquantology. 2013;11(4):591-9.
19. Şahin A, Aslan A, Baş O, İkinci A, Özyılmaz C, Sönmez FO, et al. Deleterious impacts of a 900MHz electromagnetic field on hippocampal pyramidal neurons of 8-week-old Sprague Dawley male rats. Brain Res. 2015;1624:232-8.
20. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxide in animal tissues by thiobarbutiric acid reaction. Anal Biochem. 1979;95(2):351-8.
21. Aebi H. Catalase. In: Bergmeyer HU, editor. Methods of enzymatic analysis. 2nd ed. New York: Academic Press; 1974. p.673-84.
22. Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin Chem. 1988;34(3):497-500.
23. Sedlak J, Lindsay RH. Estimation of total, protein-bound, and nonprotein sulfhydryls groups in tissue with Ellman’s reagent. Anal Biochem. 1968;25(1):192-205.
24. Hancı H, Kerimoğlu G, Mercantepe T, Odacı E. Changes in testicular morphology and oxidative stress biomarkers in 60-day-old Sprague Dawley rats following exposure to continuous 900-MHz electromagnetic field for 1 h a day throughout adolescence. Reprod Toxicol. 2018;81:71-8.
25. Okatan DÖ, Okatan AE, Hancı H, Demir S, Yaman SÖ, Çolakoğlu S, et al. Effects of 900-MHz electromagnetic fields exposure throughout middle/late adolescence on the kidney morphology and biochemistry of the female rat. Toxicol Ind Health. 2018;34(10):693-702.
26. Fragopoulou AF, Polyzos A, Papadopoulou MD, Sansone A, Manta AK, Balafas E, et al. Hippocampal lipidome and transcriptome profile alterations triggered by acute exposure of mice to GSM 1800 MHz mobile phone radiation: An exploratory study. Brain Behav. 2018;8(6):e01001.
27. Yahyazadeh A, Deniz ÖG, Kaplan AA, Altun G, Yurt KK, Davis D. The genomic effects of cell phone exposure on the reproductive system. Environ Res. 2018;167:684-93.
28. Attia AA, Yehia MA. Histological, ultrastructural and immunohistochemical studies of the low frequency electromagnetic field effect on thymus, spleen and liver of Albino Swiss mice. Pak J Biol Sci. 2002;5(9):931-7.
29. Yilmaz A, Tumkaya L, Akyildiz K, Kalkan Y, Bodur AF, Sargin F, et al. Lasting hepatotoxic effects of prenatal mobile phone exposure. J Matern Fetal Neonatal Med. 2017;30(11):1355-9.
30. Mortazavi SMJ, Mostafavi-Pour Z, Daneshmand M, Zal F, Zare R, Mosleh-Shirazi MA. Adaptive response induced by pre-exposure to 915MHz radiofrequency: a possible role for antioxidant enzyme activity. J Biomed Phys Eng. 2017;7(2):137-42.
31. Kamel OW, LeBrun DP, Berry GJ, Dorfman RF, Warnke RA. Warthin-Finkeldey polykaryocytes demonstrate a T-cell immunophenotype. Am J Clin Pathol. 1992;97(2):179-83.
32. Al-Glaib B, Al-Dardfi M, Al-Tuhami A, Elgenaidi A, Dkhil M. A technical report on the effect of electromagnetic radiation from a mobile phone on mice organs. Libyan J Med. 2008;3(1):8-9.