Annexin A2, A7 and A11 Immunohistochemical Expression in Prostate Adenocarcinoma and Relationship with Prognostic Factors

Abstract

Purpose: The role of calcium dependent phospholipase binding protein family membersAnnexin A2, A7 and A11 in the development and progression of prostate cancer was explored.Methods: Protein expressions of Annexin A2, A7 and A11 were examined byimmunohistochemistry in 53 clinicopathologically characterized prostate cancer and peritumoralareas. Difference between cancer and normal tissues was evaluated and relationship withprognostic factors was determined.Results: The expression of Annexin A2, A7 and A11, effect regulation of membrane functions,cellular homeostasis, and proliferation, were down-regulated in prostate cancer compared withadjacent non-cancerous tissues ( respectively p=0,022; 0,028; 0,033). Expression of annexina2, a7 and a11 did not correlate with age, stage, invasion of capsule and perineural tissue.Conclusion: the decrease of expression of Annexin A2, A7 and A11 protein in prostateadenocarcinoma can associate with acquired capability of invasion and loss of differentiationof tumor cells. Annexin A2 can used as immunohistochemical helper in differential diagnosisbetween normal and carcinomatous tissues on biopsy

Keywords

• Prostate adenocarcinoma,Annexin A2,Annexin A7,Annexin A11,immunohistochemistry,carcinogenesis

Prostat Adenokarsinomlarında Annexin A2, A7 Ve A11 İmmünhistokimyasal Ekspresyonu ve Prognostik Faktörlerle İlişkisi

Abstract

Amaç: Bu çalışmada kalsiyum bağımlı fosfolipid bağlayan proteinler olan Annexin ailesininüyelerinden Annexin A2, A7 ve A11’in prostat adenokarsinomu gelişimi ve ilerlemesi üzerindekirolü araştırıldı.Yöntem: 53 adet prostat adenokarsinomu olgusunda çevre normal doku ve karsinom dokularınıniyi temsil edildiği kesitlerde immünhistokimyasal olarak Annexin A2, A7 ve A11 ekspresyonu,çevre normal dokudan farkı ve tümör evresi, tümör derecesi ve diğer prognostik kriteler ileilişkisi değerlendirildi.Bulgular: Hücre homeostasisinde, membran fonksiyonlarının düzenlenmesinde ve hücreçoğalmasında etkileri olduğu bilinen Annexin A2, A7 ve A11’in normal prostat dokularındagüçlü sitoplazmik ekspresyon gösterdiği ancak prostat adenokarsinomlarında, tümörsüz çevredokuya oranla anlamlı olarak azalmış ekspresyon gösterdiği saptanmıştır ( sırası ile p=0,022;0,028; 0,033) . Gleson skor, yaş, evre, kapsül invazyonu ve perinöral invazyon ile anlamlı ilişkibulunmamıştır.Sonuç: Prostat adenokarsinom hücrelerinde gözlenen Annexin A2, A7, A11 ekspresyonazalmasının, tümör hücrelerinin diferansiasyon kaybı ve invazyon yeteneği kazanması ile ilişkiliolduğu düşünülmüştür. Ayrıca özellikle Annexin A2’nin prostat adenokarsinomunun normaldokudan ayırıcı tanısında immunhistokimyasal bir yardımcı olarak kullanılabileceğibelirlenmiştir

Keywords

• Prostat adenokarsinomu,Annexin A2,Annexin A7,Annexin A11,immunhistokimya,karsinogenez

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