Investigation of Chlamydia pneumoniae and Mycoplasma pneumoniae Antibodies in Patient with Acute Tonsillopharyngitis

Year 2008, Volume: 10 Issue: 2, 19 - 22, 01.07.2008

Fahrettin Yılmaz Oğuz Karabay Serap Köybaşı

Abstract

Aim: This study was aimed to investigate the sero-provelence of Chlamydia pneumoniae and Mycoplasma pneumoniae in patiens admitted to the ENT outpatient clinic with symptoms of acute tonsillopharangitis, and without Group A B hemolytic streptococci in throat culture. Material and Method: A total 32 subjects (aged 16 to 59, 12 male and 20 woman mean age; 34.2), who had no Group A B hemolytic streptococci in their throat culture,were included in the study. Blood was taken from patients, and serum Mycoplasma pneumoniae and Chlamydia pneumoniae antibodies were studied by ELISA with commercial tests. Result: Mycoplasma pneumoniae Ig M antibody was not detected in any subject while Chlamydia pneumoniae antibodies were positive in 6 (18.75%) subjects. Mycoplsma and Chlamydia Ig G tests were found to be positive in 6 (18.75%) and 19(59.375%) subjects, respectively. Conclusion: These results indicate that Chlamydia pneumoniae and Mycoplasma pneumoniae should be considered in patients with tonsillopharyngitis

Keywords

Mycoplasma pneumoniae, Chlamydia pneumoniae, serology, tonsillopharyngitis

Akut Tonsillofarenjitli Hastalarda Chlamydia Pneumoniae ve Mycoplasma Pneumoniae Antikorlarının Araştırılması

Abstract

Amaç: Bu çalışmada, akut tonsillofarenjit semptomlarıyla Kulak Burun Boğaz polikliniğine başvuran ve boğaz kültüründe A grubu beta hemolitik streptokok (AGBHS) saptanmayan olgularda Chlamydia pneumoniae ve Mycoplasma pneumoniae antikor sero-prevalansının araştırılması amaçlanmıştır. Gereç ve Yöntem: Çalışmaya boğaz kültüründe AGBHS saptanmayan ve yaşları 16 ile 59 arasında değişen (ort. 34.2) 12’si erkek, 20’si kadın toplam 32 olgu alındı. Olgulardan kan alınarak serum Mycoplasma pneumoniae ve Chlamydia pneumoniae antikorları mikro ELISA yöntemiyle ve ticari kitler kullanılarak çalışıldı. Bulgular: Olguların hiçbirinde M. pneumoniae Ig M pozitifliği saptanmazken 6 olguda (% 18.75) C. pneumoniae Ig M pozitifliği saptandı. Mycoplasma ve Chlamydia Ig G pozitifliği sırasıyla 6 (% 18.75) ve 19 (% 59.375) olguda saptandı. Sonuç: Bu sonuçlar Chlamydia pneumoniae ve Mycoplasma pneumoniae’nın akut tonsillofarenjit etiyolojisinde göz önünde bulundurulması gerektiğini göstermektedir

Keywords

Mycoplasma pneumoniae, Chlamydia pneumoniae, seroloji, tonsillofarenjit

References

• Meijer A, Dagnelie CF, De Jong JC, De Vries A, Bestebroer TM, Van Loon AM, Bartelds AI, Ossewaarde JM. Low prevalence of Chlamydia pneumoniae and Mycoplasma pneumoniae among patients with symptoms of respiratory tract infections in Dutch general practices. Eur J Epidemiol. 16:1099- 106, 2000.
• Kuo CC, Jackson LA, Campbell LA, Grayston JT. Chlamydia pneumoniae (TWAR). Clin Microbiol Rev. 8:451-61, 1995.
• Clyde WA Jr. Clinical overview of typical Mycoplasma pneumoniae infections. Clin Infect Dis. 17 Suppl 1:32-6, 1993.
• Kaygusuz S, Koksal I, Aydin K, Caylan R. Investigation of atypical bacteria and virus antigens in respiratory tract infections by use of an immunofluorescence method. Jpn J Infect Dis. 57:33–6, 2004.
• Fong CK, Lee MK, Griffith BP. Evaluation of R-Mix FreshCells in shell vials for detection of respiratory viruses. J Clin Microbiol. 38:4660–2, 2000.
• Gröndahl B, Puppe W, Hoppe A, Kühne I, Weigl JA, Schmitt HJ. Rapid identification of nine microorganisms causing acute respiratory tract infections by single-tube multiplex reverse transcription-PCR: feasibility study. J Clin Microbiol. 37:1–7, 1999.
• Reina J, Ros MJ, Del Valle JM, Blanco I, Munar M. Evaluation of direct immunofluorescence, dot-blot enzyme immunoassay, and shell-vial culture for detection of respiratory syncytial virus in patients with bronchiolitis. Eur J Clin Microbiol Infect Dis. 14.1018 – 20, 1995.
• Thom DH, Grayston JT, Campbell LA, Kuo CC, Diwan VK, Wang SP. Respiratory infection with Chlamydia pneumoniae in middle-aged and older adult outpatients. Eur J Clin Microbiol Infect Dis. 13:785–92, 1994.
• Grayston JT. Infections caused by Chlamydia pneumoniae strain TWAR. Clin Infect Dis. 15:757–61, 1992
• Hammerschlag MR, Chirgwin K, Roblin PM, Gelling M, Dumornay W, Mandel L, Smith P, Schachter J. Persistent infection with Chlamydia pneumoniae following acute respiratory illness. Clin Infect Dis. 14:178–82, 1992 .
• Esposito S, Bosis S, Begliatti E, Droghetti R, Tremolati E, Tagliabue C, Bellasio M, Blasi F, Principi N. Acute tonsillopharyngitis associated with atypical bacterial infection in children: natural history and impact of macrolide therapy. Clin Infect Dis. 15; 43: 206-9, 2006.
• Schelhamer JH, Gill VJ, Quinn TC,et al. The laboratory evaluation of opportunis- tic pulmonary infections. Ann Int Med 1996; 124: 585.