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Protective Effect of Urtica dioica on Liver Injury Induced by Hepatic Ischemia Reperfusion Injury in Rats

Year 2010, Volume: 12 Issue: 1, 43 - 47, 01.03.2010

Abstract

Background: This study was designed to investigate the effects of Urtica dioica on liverischemia reperfusion injury in rats. Methods: Thirty male Wistar-albino rats were used in this experimental study. Animals weredivided into three groups as sham operated (group 1), control (group 2), and Urtica dioicatreatment group (group 3). Urtica dioica 2ml/kg were administered intraperitoneally beforeischemia and immediately after the reperfusion. The levels of total antioxidant capacity, totalfree sulfidril group, Total oxidant status, Oxidative stress index, and myeloperoxidase in livertissues were measured. The serum levels of ALT, AST and LDH were also measuredResults: Total antioxidant capacity and total free sulfidril group in liver tissue were significantlyhigher in group 3 than in group 2. Oxidative stress index and myeloperoxidase in liver tissuewere significantly lower in group 3 than the group 2. The levels of liver enzymes in treatmentgroup were significantly lower than those in the control group. Histological tissue damage wasmilder in the treatment group than that in the control group.Conclusion: It is concluded that Urtica dioica increase the antioxidant capacity and decreaseoxidative stress and liver enzymes in the hepatic ischemi reperfusion injury of rats

References

  • Toledo-Pereyra LH, Simmons RL, Najarian JS: Protection of the ischemic liver by donor pretreatment before transplantation. Am J Surg. 129:513–7, 1975.
  • Kaplowitz N. Mechanisms of liver injury. J Hepatol. 32:39– 47, 2000.
  • Jaeschke H: Molecular mechanisms of hepatic ischemia- reperfusion injury and preconditioning. Am J Physiol-Gastrointest Liver Physiol. 284:G15–G26, 2003.
  • Gülçin I, Küfrevioglu OI, Oktay M, Büyükokuroglu ME: Antioxidant, antimicrobial, antiulcer and analgesic activities of nettle (Urtica dioica L.). J Ethnopharmacol. 90: 205– 215, 2004.
  • Mittman P: Randomized, double-blind study of freeze-dried Urtica dioica in the treatment of allergic rhinitis. Planta Med. 56:44-47, 1990.
  • Turkdogan MK, Ozbek H, Yener Z, Tuncer I, Uygan I, Ceylan E: The role of Urtica dioica and Nigella sativa in the prevention of carbon tetrachloride induced hepatotoxicity in rats. Phytother Res. 17: 942–946, 2003.
  • Kanter M, Coskun O, Budancamanak M: Hepatoprotective effects of Nigella sativa L. and Urtica dioica L. on lipid peroxidation, antioxidant enzyme systems and liver enzymes in carbon tetrachloride-treated rats. World J Gastroenterol. 42: 6684–6688, 2005.
  • Erel O: A novel automated method to measure total antioxidant response against potent free radical reactions. Clin Biochem. 37:112–9, 2004.
  • Erel O: A new automated colorimetric method for measuring total oxidant status. Clin Biochem. 38:1103–11, 2005.
  • Bolukbas C, Bolukbas FF, Horoz M, Aslan M, Celik H, Erel O: Increased oxidative stress associated with the severity of the liver disease in various forms of hepatitis B virus infection. BMC Infect Dis. 5:95, 2005.
  • Wei H, Frenkel K: Relationship of oxidative events and DNA oxidation in sencar mice to in vivo promoting activity of phorbol ester-type tumor promoters. Carcinogenesis. 14:1195– 1201, 1993.
  • Hu ML, Louie S, Cross CE, Motchnik P, Halliwell B: Antioxidant protection against hypochlorous acid in human plasma. J Lab Clin Med. 121:257-62, 1993.
  • Colletti LM, Kunkel SL, Walz A, Burdick MD, Kunkel RG, Wilke CA et al: The role of cytokine networks in the local liver injury following hepatic ischemia/reperfusion in the rat. Hepatology. 23:506–14, 1996.
  • Jaeschke H, Farhood A: Neutrophil and Kupffer cell-induced oxidant stress and ischemia-reperfusion injury in rat liver. Am J Physiol. 260(3 pt 1):G355–62, 1991.
  • Yuan GJ, Ma JC, Gong ZJ, Sun XM, Zheng SH, Li X: Modulation of liver oxidant-antioxidant system by ischemic preconditioning during ischemia/reperfusion injury in rats. World J Gastroenterol. 11:1825-1828, 2005.
  • Zhang SJ, Shi JH, Tang Z, Wu Y, Chen S: Protective effects of glycine pretreatment on brain-death donor liver. Hepatobiliary Pancreat Dis Int 4:37-40, 2005.
  • Özbek H, Tuncer İ, Dülger H, Uğraş S, Bayram İ, Türkdoğan K, Uygan İ: E Vitamini, N-Asetil Sistein, Penisilin-G ve Urtica dioica L.’nin Phalloidin Toksisitesi Üzerine Etkileri. Van Tıp Dergisi. 12(1);16-21, 2005.
  • Keles MS, Demirci N, Yildirim A, Atamanalp SS, Altinkaynak K: Protective effects of N-acetylcysteine and Ginkgo biloba extract on ischaemia-reperfusion-induced hepatic DNA damage in rats. Clin Exp Med. 8(4):193-8, 2008.
  • Gong J, Lao XJ, Zhang SJ, Chen S: Protective effects of L- arginine against ischemia-reperfusion injury in non-heart beating rat liver graft. Hepatobiliary Pancreat Dis Int. 7(5):481- 4, 2008.
  • Zapletal C, Heyne S, Breitkreutz R, Gebhard MM, Golling M: The influence of selenium substitution on microcirculation and glutathione metabolism after warm liver ischemia/reperfusion in a rat model. Microvasc Res. 76(2):104-9, 2008.
  • Toldy A, Stadler K, Sasvári M, Jakus J, Jung KJ, Chung HY, Berkes I, Nyakas C, Radák Z: The effect of exercise and nettle supplementation on oxidative stress markers in the rat brain. Brain Res Bull. 65(6):487-93, 2005.

Karaciğer İskemi Reperfüzyon Hasarı Yapılan Sıçanlarda Urtica dioica’nın Karaciğer Üzerine Koruyucu Etkisi

Year 2010, Volume: 12 Issue: 1, 43 - 47, 01.03.2010

Abstract

Amaç: Karaciğer iskemi reperfüzyon hasarı yapılan sıçanlarda Urtica dioica’nın karaciğerdokusuna koruyucu etkilerini araştırmak amacıyla bu çalışmayı planladık. Gereç ve Yöntem: Çalışmamızda toplam 30 adet Wistar-albino sıçan kullanıldı. Sıçanlar, operesham grup (Grup1), kontrol grup (Grup2) ve Urtica dioica tedavi grubu(Grup3) olarak 3 eşitgruba ayrıldı. Tedavi grubuna iskemiden hemen önce ve reperfüzyondan hemen sonra 2 ml/kgintraperitoneal olarak Urtica dioica verildi. Karaciğer dokusunda total antioksidan kapasite, totalfree sülfidril grup, total oksidan durum, oksidatif stress indeksi ve myeloperoksidaz seviyeleriölçüldü. Ayrıca serumda ALT, AST ve LDH seviyeleri ölçüldü.Bulgular: Karaciğer dokusunda total antioksidan kapasite ve total free sülfidril grup değerleritedavi grubunda kontrol grubuna göre anlamlı derecede artmıştı. (p

References

  • Toledo-Pereyra LH, Simmons RL, Najarian JS: Protection of the ischemic liver by donor pretreatment before transplantation. Am J Surg. 129:513–7, 1975.
  • Kaplowitz N. Mechanisms of liver injury. J Hepatol. 32:39– 47, 2000.
  • Jaeschke H: Molecular mechanisms of hepatic ischemia- reperfusion injury and preconditioning. Am J Physiol-Gastrointest Liver Physiol. 284:G15–G26, 2003.
  • Gülçin I, Küfrevioglu OI, Oktay M, Büyükokuroglu ME: Antioxidant, antimicrobial, antiulcer and analgesic activities of nettle (Urtica dioica L.). J Ethnopharmacol. 90: 205– 215, 2004.
  • Mittman P: Randomized, double-blind study of freeze-dried Urtica dioica in the treatment of allergic rhinitis. Planta Med. 56:44-47, 1990.
  • Turkdogan MK, Ozbek H, Yener Z, Tuncer I, Uygan I, Ceylan E: The role of Urtica dioica and Nigella sativa in the prevention of carbon tetrachloride induced hepatotoxicity in rats. Phytother Res. 17: 942–946, 2003.
  • Kanter M, Coskun O, Budancamanak M: Hepatoprotective effects of Nigella sativa L. and Urtica dioica L. on lipid peroxidation, antioxidant enzyme systems and liver enzymes in carbon tetrachloride-treated rats. World J Gastroenterol. 42: 6684–6688, 2005.
  • Erel O: A novel automated method to measure total antioxidant response against potent free radical reactions. Clin Biochem. 37:112–9, 2004.
  • Erel O: A new automated colorimetric method for measuring total oxidant status. Clin Biochem. 38:1103–11, 2005.
  • Bolukbas C, Bolukbas FF, Horoz M, Aslan M, Celik H, Erel O: Increased oxidative stress associated with the severity of the liver disease in various forms of hepatitis B virus infection. BMC Infect Dis. 5:95, 2005.
  • Wei H, Frenkel K: Relationship of oxidative events and DNA oxidation in sencar mice to in vivo promoting activity of phorbol ester-type tumor promoters. Carcinogenesis. 14:1195– 1201, 1993.
  • Hu ML, Louie S, Cross CE, Motchnik P, Halliwell B: Antioxidant protection against hypochlorous acid in human plasma. J Lab Clin Med. 121:257-62, 1993.
  • Colletti LM, Kunkel SL, Walz A, Burdick MD, Kunkel RG, Wilke CA et al: The role of cytokine networks in the local liver injury following hepatic ischemia/reperfusion in the rat. Hepatology. 23:506–14, 1996.
  • Jaeschke H, Farhood A: Neutrophil and Kupffer cell-induced oxidant stress and ischemia-reperfusion injury in rat liver. Am J Physiol. 260(3 pt 1):G355–62, 1991.
  • Yuan GJ, Ma JC, Gong ZJ, Sun XM, Zheng SH, Li X: Modulation of liver oxidant-antioxidant system by ischemic preconditioning during ischemia/reperfusion injury in rats. World J Gastroenterol. 11:1825-1828, 2005.
  • Zhang SJ, Shi JH, Tang Z, Wu Y, Chen S: Protective effects of glycine pretreatment on brain-death donor liver. Hepatobiliary Pancreat Dis Int 4:37-40, 2005.
  • Özbek H, Tuncer İ, Dülger H, Uğraş S, Bayram İ, Türkdoğan K, Uygan İ: E Vitamini, N-Asetil Sistein, Penisilin-G ve Urtica dioica L.’nin Phalloidin Toksisitesi Üzerine Etkileri. Van Tıp Dergisi. 12(1);16-21, 2005.
  • Keles MS, Demirci N, Yildirim A, Atamanalp SS, Altinkaynak K: Protective effects of N-acetylcysteine and Ginkgo biloba extract on ischaemia-reperfusion-induced hepatic DNA damage in rats. Clin Exp Med. 8(4):193-8, 2008.
  • Gong J, Lao XJ, Zhang SJ, Chen S: Protective effects of L- arginine against ischemia-reperfusion injury in non-heart beating rat liver graft. Hepatobiliary Pancreat Dis Int. 7(5):481- 4, 2008.
  • Zapletal C, Heyne S, Breitkreutz R, Gebhard MM, Golling M: The influence of selenium substitution on microcirculation and glutathione metabolism after warm liver ischemia/reperfusion in a rat model. Microvasc Res. 76(2):104-9, 2008.
  • Toldy A, Stadler K, Sasvári M, Jakus J, Jung KJ, Chung HY, Berkes I, Nyakas C, Radák Z: The effect of exercise and nettle supplementation on oxidative stress markers in the rat brain. Brain Res Bull. 65(6):487-93, 2005.
There are 21 citations in total.

Details

Primary Language Turkish
Journal Section Research Article
Authors

Fahrettin Yıldız This is me

Sacit Çoban This is me

Abdullah Taşkın This is me

Muharrem Bitiren This is me

Nurten Aksoy This is me

Alpaslan Terzi This is me

Publication Date March 1, 2010
Published in Issue Year 2010 Volume: 12 Issue: 1

Cite

APA Yıldız, F., Çoban, S., Taşkın, A., Bitiren, M., et al. (2010). Karaciğer İskemi Reperfüzyon Hasarı Yapılan Sıçanlarda Urtica dioica’nın Karaciğer Üzerine Koruyucu Etkisi. Duzce Medical Journal, 12(1), 43-47.
AMA Yıldız F, Çoban S, Taşkın A, Bitiren M, Aksoy N, Terzi A. Karaciğer İskemi Reperfüzyon Hasarı Yapılan Sıçanlarda Urtica dioica’nın Karaciğer Üzerine Koruyucu Etkisi. Duzce Med J. March 2010;12(1):43-47.
Chicago Yıldız, Fahrettin, Sacit Çoban, Abdullah Taşkın, Muharrem Bitiren, Nurten Aksoy, and Alpaslan Terzi. “Karaciğer İskemi Reperfüzyon Hasarı Yapılan Sıçanlarda Urtica dioica’nın Karaciğer Üzerine Koruyucu Etkisi”. Duzce Medical Journal 12, no. 1 (March 2010): 43-47.
EndNote Yıldız F, Çoban S, Taşkın A, Bitiren M, Aksoy N, Terzi A (March 1, 2010) Karaciğer İskemi Reperfüzyon Hasarı Yapılan Sıçanlarda Urtica dioica’nın Karaciğer Üzerine Koruyucu Etkisi. Duzce Medical Journal 12 1 43–47.
IEEE F. Yıldız, S. Çoban, A. Taşkın, M. Bitiren, N. Aksoy, and A. Terzi, “Karaciğer İskemi Reperfüzyon Hasarı Yapılan Sıçanlarda Urtica dioica’nın Karaciğer Üzerine Koruyucu Etkisi”, Duzce Med J, vol. 12, no. 1, pp. 43–47, 2010.
ISNAD Yıldız, Fahrettin et al. “Karaciğer İskemi Reperfüzyon Hasarı Yapılan Sıçanlarda Urtica dioica’nın Karaciğer Üzerine Koruyucu Etkisi”. Duzce Medical Journal 12/1 (March 2010), 43-47.
JAMA Yıldız F, Çoban S, Taşkın A, Bitiren M, Aksoy N, Terzi A. Karaciğer İskemi Reperfüzyon Hasarı Yapılan Sıçanlarda Urtica dioica’nın Karaciğer Üzerine Koruyucu Etkisi. Duzce Med J. 2010;12:43–47.
MLA Yıldız, Fahrettin et al. “Karaciğer İskemi Reperfüzyon Hasarı Yapılan Sıçanlarda Urtica dioica’nın Karaciğer Üzerine Koruyucu Etkisi”. Duzce Medical Journal, vol. 12, no. 1, 2010, pp. 43-47.
Vancouver Yıldız F, Çoban S, Taşkın A, Bitiren M, Aksoy N, Terzi A. Karaciğer İskemi Reperfüzyon Hasarı Yapılan Sıçanlarda Urtica dioica’nın Karaciğer Üzerine Koruyucu Etkisi. Duzce Med J. 2010;12(1):43-7.