Oxidative Tissue Damage in the Prefrontal Cortex of Pinealectomized Rats and Protective Effects of Melatonin Hormone

Year 2012, Volume: 14 Issue: 1, 37 - 41, 01.03.2012

Murat Abdülgani Kuş Serdar Çolakoğlu Burak Gülcen İlter Kuş Mustafa Sarsılmaz Mehmet Akif Ersoy Ömür Karaca

Abstract

Aim: An attempt was made to investigate the tissue samples of prefrontal cortex frompinealectomized rats and melatonin applied rats following pinealectomy in biochemical basis.Materials and Methods: Twenty-one, male Wistar rats were divided equally into three groups.Rats in Group I were used as control (sham-pinealectomy). Rats in Group II werepinealectomized. Rats in Group III were injected daily with melatonin (1 mg/kg/)intraperitoneally for 3 months following pinealectomy. At the end of the experimental period,all rats were killed by decapitation and the prefrontal cortexes of rats were removed. Superoxidedismutase (SOD), glutathione peroxidase (GSH-Px) enzyme activities and malondialdehyde(MDA) levels were determined at biochemical levels in the prefrontal cortex specimens.Results: The levels of SOD and GSH-Px in the prefrontal cortex tissue were significantlydecreased and MDA levels were significantly increased in pinealectomized rats compared withthose of the controls. However, increased activities of SOD and GSH-Px enzymes and decreasedlevels of MDA were detected in the rats administered melatonin following pinealectomy.Conclusion: As a result of this study, pinealectomy-induced oxidative damage in the prefrontalcortex tissue in rats were prevented by administration of melatonin hormone

Keywords

Prefrontal cortex, melatonin, pinealectomy, rat

Pinealektomili Sıçanlarda Prefrontal Kortekste Oluşan Oksidatif Doku Hasarı Ve Melatonin Hormonunun Koruyucu Etkisi

Abstract

Amaç: Çalışmamızda, pinealektomili ve pinealektomi sonrası melatonin uygulanan sıçanlaraait prefrontal korteks doku örneklerinin biyokimyasal düzeyde incelenmesi amaçlandı.Gereç ve Yöntem: 21 adet Wistar-Albino cinsi erkek sıçanlar üç eşit gruba ayrıldı. Grup I’dekisıçanlar kontrol (sham-pinealektomi) olarak kullanıldı. Grup II’ye ait hayvanlara cerrahi olarakpinealektomi yapıldı. Grup III’deki sıçanlara ise pinealektomi sonrası günlük olarak, üç ayboyunca, melatonin hormonu (1 mg/kg/) intraperitoneal yoldan enjekte edildi. Deney süresisonunda bütün sıçanlar dekapite edilerek öldürüldü ve prefrontal korteksleri çıkartıldı. Prefrontalkorteks doku örneklerinde süperoksit dismutaz (SOD), glutatyon peroksidaz (GSH-Px) enzimaktiviteleri ile malondialdehit (MDA) seviyeleri biyokimyasal olarak belirlendi.Bulgular: Pinealektomili sıçanlarda, prefrontal korteks SOD ve GSH-Px enzim değerlerininkontrol grubuna göre anlamlı bir şekilde azaldığı, MDA düzeylerinin ise yine istatistiksel olarakanlamlı bir şekilde arttığı tespit edildi. Pinealektomi sonrası melatonin enjekte edilen sıçanlardaise, doku SOD ve GSH-Px enzim aktivitelerinin yükseldiği, MDA seviyelerinin de azaldığıtespit edildi.Sonuç: Bu çalışma sonucunda, sıçanlarda pinealektomiye bağlı olarak prefrontal korteks’temeydana gelen oksidatif doku hasarının melatonin enjeksiyonu ile önlendiği tespit edildi

Keywords

Prefrontal korteks, melatonin, pinealektomi, sıçan

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