ERYTHROPOIETIN ACCELERATES FRACTURE HEALING AN EXPERIMENTAL ANIMAL STUDY

Year 2014, Volume: 16 Issue: 1, 8 - 11, 01.03.2014

Erdinç Türkeli Mustafa Uslu Ali Doğan

Abstract

Aim: The efficacy of erythropoietin (EPO) on wound healing has been shown before. There islimited data about the efficacy of EPO on fracture healing. In this study, the efficacy of EPO onclosed forearm fracture healing is searched by an experimental model. Material and method: Twenty eight rats were picked randomly and divided into EPO andControl groups. On the first day, fracture was performed on the right ulna and radius of all rats.500 U/kg daily low dose EPO was administered for each rat in EPO group and the same volumeof isotonic sodium chloride solution was given intraperitoneally in the control group for fivedays. Seven rats from each group were sacrificed at the end of the first week; the others weresacrificed at the end of the third week. Results: At the end of the first week and third week, bone healing scores of EPO group issignificantly higher than the control group according to the histological, clinic and radiologicanalysis. Conclusion: EPO could have positive effects on healing of forearm fracture on rats in acuteand subacute period. Low dose EPO may be a new protective agent against delayed union ornonunion in the risk population to go or not to go to surgical treatment

Keywords

Erythropoietin, fracture healing, rat

Eritropoetin Kırık İyileşmesini Hızlandırıyor Deneysel Hayvan Çalışması

Abstract

Giriş: Eritropoetin’in yara iyileşmesi üzerine olan etkisi daha önce gösterilmiştir. Kırıkiyileşmesi üzerine olan etkisi üzerine sınırlı sayıda bilgi mevcuttur. Bu çalışmada eritropoetininkapalı kırık iyileşmesi üzerine olan etkileri deneysel bir model kullanılarak araştırılmıştır.Yöntem: Yirmisekiz rat alınıp rastgele sçilerek Eritropoetin ve Kontrol grupları oluşturuldu.Çalışmanın ilk gününde tüm ratların sağ önkollarında kapalı ulna ve radius cisim kırıklarıoluşturuldu. Beş gün boyunca Eritropoetin grubundaki ratlarda 500 U/kg düşük doz eritropoetinintraperitoneal olarak verilirken; Kontrol grubundaki ratlarda ise aynı hacimde izotonikintraperitoneal olarak verildi. Gruplardaki yedişer det rat ilk hafta sonunda, kalan yedişer adetratda üçüncü haftanın sonunda sakrifiye edildi.Bulgular: Birinci ve üçüncü hafta sonunda kırık iyileşme skorları histolojik, radyolojik ve klinikolarak değerlendirildiğinde Eritropoetin grubunda Kontrol grubuna gore belirgin olarak dahayüksek olduğu saptandı.Sonuç: Ratların kapalı önkol kırıklarının iyileşmesi üzerine akut ve subakut peryottaeritropoetinin pozitif etkileri olabilir. Düşük doz eritropoetin kullanımı cerrahiye gidecek veyagitmeyecek olan riskli hasta gruplarında kaynama gecikmesi veya kaynamamaya karşı koruyucuajan olarak verilebilir

Keywords

Eritropoetin, kırık iyileşmesi, rat

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