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Deneysel Sinirbilim Araştırmalarında Kullanılan Hayvan Deneyleri: Öğrenme, Hafıza, Anksiyete, Depresyon ve Motor Fonksiyon Davranış Deneyleri

Year 2024, Volume: 26 Issue: S1, 65 - 71, 30.06.2024
https://doi.org/10.18678/dtfd.1493348

Abstract

Davranış deneyleri, 1904'te Ivan Pavlov'un klasik koşullanma araştırmalarından beri yapılmaktadır. Hastalıkların mekanizmalarının anlaşılması, bu hastalıkların önlenmesi ve etkin tedavi yöntemlerinin geliştirilmesinde, deneysel çalışmalar önemli rol oynar. Hayvan modelleri kullanılarak yapılan araştırmalar, bu hastalıkların mekanizmalarını aydınlatabilmek ve etkin tedavi stratejileri geliştirebilmek için oldukça önemlidir. Bir tedavi yönteminin araştırılmasında, yeni tedavi protokollerinin geliştirilmesinde ve yeni ilaç moleküllerinin keşfedilmesinde, hayvan modelleri yaygın olarak kullanılır. Geliştirilecek olan ilacın etkinliği hem araştırılmaya başlanmadan önce hayvan modelinin oluşup oluşmadığının test edilmesi için hem de ilacın tedavideki etkinliği açısından ve hem de araştırılacak mekanizmaların aydınlatılması için oldukça önemlidir. Bundan dolayı genellikle davranış deneyleri ile değerlendirmeler yapılır. Her davranış deneyinin kendine özgü avantajları ve dezavantajları vardır. Bu nedenle araştırmacı, davranış deneyini seçmeden önce bu avantajların ve kısıtlamaların farkında olarak en uygun davranış deneyini tercih etmelidir. Bu derlemede Alzheimer, epilepsi, migren, nöropatik ağrı, şizofreni, Parkinson, serebral iskemi ve travmatik beyin hasarı gibi deneysel modellerde en sık kullanılan öğrenme, hafıza, anksiyete, depresyon ve motor fonksiyon davranışsal deney protokollerinin detaylı olarak tanımlanması amaçlandı.

References

  • Bovenkerk B, Kaldewaij F. The use of animal models in behavioural neuroscience research. Curr Top Behav Neurosci. 2015;19:17-46.
  • Agar A, Akcay G. Nörolojik hastaliklarin deneysel hayvan modelleri, vol 1. Ankara: Akademisyen Kitabevi; 2022. Turkish.
  • Akcay G, Derin N. The effects of tDCS on depression and anxiety disorders induced by sub-chronic stress. Turk Hij Den Biyol Derg. 2022;79(2):267-78.
  • Akcay G, Nemutlu Samur D, Derin N. Transcranial direct current stimulation alleviates nociceptive behavior in male rats with neuropathic pain by regulating oxidative stress and reducing neuroinflammation. J Neurosci Res. 2023;101(9):1457-70.
  • Akçay, G. Weight drop models in traumatic brain injury. Mid Blac Sea J Health Sci. 2023;9(2):375-84.
  • Akcay G. Therapeutic effects of transcranial direct current stimulation on ketamine-induced schizophrenia-like behaviors and oxidative stress. Med Science. 2023;12(1):63-9.
  • Akcay G, Tamerer S. Transcranial direct current stimulation ameliorates motor and cognitive functions by regulating neuronal excitotoxicity in experimental Parkinson’s disease model. Cukurova Med J. 2023;48(3):919-28.
  • Akcay G, Baydemir R. Therapeutic effects of transcranial direct current stimulation on loss of motor function caused by experimental mild traumatic brain injury. Cukurova Med J. 2023;48(3):972-8.
  • Akçay G, Aslan M, Kipmen Korgun D, Çeker T, Akan E, Derin N. Effects of transcranial direct current stimulation on the glutamatergic pathway in the male rat hippocampus after experimental focal cerebral ischemia. J Neurosci Res. 2024;102(1):e25247.
  • Nemutlu Samur D, Akçay G, Yıldırım S, Özkan A, Çeker T, Derin N, et al., Vortioxetine ameliorates motor and cognitive impairments in the rotenone-induced Parkinson's disease via targeting TLR-2 mediated neuroinflammation. Neuropharmacology, 2022;208:108977.
  • Danışman B, Akçay G, Gökçek-Saraç Ç, Kantar D, Aslan M, Derin N. The role of acetylcholine on the effects of different doses of sulfite in learning and memory. Neurochem Res. 2022;47(11):3331-43.
  • Özkan A, Bülbül M, Derin N, Sinen O, Akçay G, Parlak H, et al. Neuropeptide-S affects cognitive impairment and depression-like behavior on MPTP induced experimental mouse model of Parkinson’s disease. Turk J Med Sci. 2021;51(6):3126-35.
  • Johnston IN, Tan M, Cao J, Matsos A, Forrest DRL, Si E, et al. Ibudilast reduces oxaliplatin-induced tactile allodynia and cognitive impairments in rats. Behav Brain Res. 2017;334:109-18.
  • Wagner BA, Braddick VC, Batson CG, Cullen BH, Miller LE, Spritzer MD. Effects of testosterone dose on spatial memory among castrated adult male rats. Psychoneuroendocrinology. 2018;89:120-30.
  • Akcay G, Bahadir A, Tatar Y, Nuri Atalar M, Babur C, Taylan Ozkan A. Investigation of the effects of Toxoplasma gondii on behavioral and molecular mechanism in bradyzoite stage. Brain Res. 2024;1828:148762.
  • Zhao Y, Li H, Fang F, Qin T, Xiao W, Wang Z, Ma S. Geniposide improves repeated restraint stress-induced depression-like behavior in mice by ameliorating neuronal apoptosis via regulating GLP-1R/AKT signaling pathway. Neurosci Lett. 2018;676:19-26.
  • Kawashita E, Kanno Y, Ikeda K, Kuretake H, Matsuo O, Matsuno H. Altered behavior in mice with deletion of the alpha2-antiplasmin gene. PLoS One. 2014;9(5):e97947.
  • Plekanchuk VS, Prokudina OI, Ryazanova MA. Social behavior and spatial orientation in rat strains with genetic predisposition to catatonia (GC) and stereotypes (PM). Vavilovskii Zhurnal Genet Selektsii. 2022;26(3):281-9.
  • Cioato SG, Medeiros LF, Marques Filho PR, Vercelino R, de Souza A, Scarabelot VL, et al. Long-lasting effect of transcranial direct current stimulation in the reversal of hyperalgesia and cytokine alterations induced by the neuropathic pain model. Brain Stimul. 2016;9(2):209-17.
  • Santos DS, Lopes BC, Medeiros LF, Assumpção JAF, de Souza A, Salvi AA, et al. Transcranial direct current stimulation (tDCS) induces analgesia in rats with neuropathic pain and alcohol abstinence. Neurochem Res. 2020;45(11):2653-63.
  • Hacısüleyman L, Saraç B, Joha Z. Analgesic effects of vilazodone, indatraline, and talsupram in a rat model of neuropathic pain. Turk J Pharm Sci. 2022;19(3):336-42.
  • Arslan R, Aydin S, Nemutlu Samur D, Bektas N. The possible mechanisms of protocatechuic acid-induced central analgesia. Saudi Pharm J. 2018;26(4):541-5.
  • Zhang Y, Ma S, Ke X, Yi Y, Yu H, Yu D, Li Q, Shang Y, Lu Y, Pei L. The mechanism of Annexin A1 to modulate TRPV1 and nociception in dorsal root ganglion neurons. Cell Biosci. 2021;11(1):167.
  • De la Luz-Cuellar YE, Rodríguez-Palma EJ, Franco-Enzástiga Ú, Déciga-Campos M, Mercado F, Granados-Soto V. Spinal dopaminergic D1 and D5 receptors contribute to reserpine-induced fibromyalgia-like pain in rats. Brain Res. 2023;1799:148167.
  • Girotra P, Singh SK. A comparative study of orally delivered PBCA and ApoE coupled BSA nanoparticles for brain targeting of sumatriptan succinate in therapeutic management of migraine. Pharm Res. 2016;33(7):1682-95.

Animal Experiments Used in Experimental Neuroscience Research: Learning, Memory, Anxiety, Depression and Motor Function Behavioural Experiments

Year 2024, Volume: 26 Issue: S1, 65 - 71, 30.06.2024
https://doi.org/10.18678/dtfd.1493348

Abstract

Behavioral experiments have been conducted since the classical conditioning research of Ivan Pavlov in 1904. Experimental research plays an important role in understanding the mechanisms of diseases, preventing these diseases, and developing effective treatment methods. Research using animal models is very important to understand the mechanisms of these diseases and to develop effective treatment strategies. Animal models are widely used in the research of a treatment method, the development of novel treatment protocols, and the discovery of new drug molecules. The efficacy of the drug to be developed is very important both for testing whether the animal model is formed before starting the research and for the effectiveness of the drug in treatment and for the elucidation of the mechanisms to be investigated. Therefore, evaluations are usually made with behavioral experiments. Each behavioral experiment has its own advantages and disadvantages. Therefore, the researcher should be aware of these advantages and limitations before choosing the most appropriate behavioral experiment. This review aimed to describe the most commonly used learning, memory, anxiety, depression, and motor function behavioral experimental protocols in experimental models such as Alzheimer's, epilepsy, migraine, neuropathic pain, schizophrenia, Parkinson's, cerebral ischemia, and traumatic brain injury.

References

  • Bovenkerk B, Kaldewaij F. The use of animal models in behavioural neuroscience research. Curr Top Behav Neurosci. 2015;19:17-46.
  • Agar A, Akcay G. Nörolojik hastaliklarin deneysel hayvan modelleri, vol 1. Ankara: Akademisyen Kitabevi; 2022. Turkish.
  • Akcay G, Derin N. The effects of tDCS on depression and anxiety disorders induced by sub-chronic stress. Turk Hij Den Biyol Derg. 2022;79(2):267-78.
  • Akcay G, Nemutlu Samur D, Derin N. Transcranial direct current stimulation alleviates nociceptive behavior in male rats with neuropathic pain by regulating oxidative stress and reducing neuroinflammation. J Neurosci Res. 2023;101(9):1457-70.
  • Akçay, G. Weight drop models in traumatic brain injury. Mid Blac Sea J Health Sci. 2023;9(2):375-84.
  • Akcay G. Therapeutic effects of transcranial direct current stimulation on ketamine-induced schizophrenia-like behaviors and oxidative stress. Med Science. 2023;12(1):63-9.
  • Akcay G, Tamerer S. Transcranial direct current stimulation ameliorates motor and cognitive functions by regulating neuronal excitotoxicity in experimental Parkinson’s disease model. Cukurova Med J. 2023;48(3):919-28.
  • Akcay G, Baydemir R. Therapeutic effects of transcranial direct current stimulation on loss of motor function caused by experimental mild traumatic brain injury. Cukurova Med J. 2023;48(3):972-8.
  • Akçay G, Aslan M, Kipmen Korgun D, Çeker T, Akan E, Derin N. Effects of transcranial direct current stimulation on the glutamatergic pathway in the male rat hippocampus after experimental focal cerebral ischemia. J Neurosci Res. 2024;102(1):e25247.
  • Nemutlu Samur D, Akçay G, Yıldırım S, Özkan A, Çeker T, Derin N, et al., Vortioxetine ameliorates motor and cognitive impairments in the rotenone-induced Parkinson's disease via targeting TLR-2 mediated neuroinflammation. Neuropharmacology, 2022;208:108977.
  • Danışman B, Akçay G, Gökçek-Saraç Ç, Kantar D, Aslan M, Derin N. The role of acetylcholine on the effects of different doses of sulfite in learning and memory. Neurochem Res. 2022;47(11):3331-43.
  • Özkan A, Bülbül M, Derin N, Sinen O, Akçay G, Parlak H, et al. Neuropeptide-S affects cognitive impairment and depression-like behavior on MPTP induced experimental mouse model of Parkinson’s disease. Turk J Med Sci. 2021;51(6):3126-35.
  • Johnston IN, Tan M, Cao J, Matsos A, Forrest DRL, Si E, et al. Ibudilast reduces oxaliplatin-induced tactile allodynia and cognitive impairments in rats. Behav Brain Res. 2017;334:109-18.
  • Wagner BA, Braddick VC, Batson CG, Cullen BH, Miller LE, Spritzer MD. Effects of testosterone dose on spatial memory among castrated adult male rats. Psychoneuroendocrinology. 2018;89:120-30.
  • Akcay G, Bahadir A, Tatar Y, Nuri Atalar M, Babur C, Taylan Ozkan A. Investigation of the effects of Toxoplasma gondii on behavioral and molecular mechanism in bradyzoite stage. Brain Res. 2024;1828:148762.
  • Zhao Y, Li H, Fang F, Qin T, Xiao W, Wang Z, Ma S. Geniposide improves repeated restraint stress-induced depression-like behavior in mice by ameliorating neuronal apoptosis via regulating GLP-1R/AKT signaling pathway. Neurosci Lett. 2018;676:19-26.
  • Kawashita E, Kanno Y, Ikeda K, Kuretake H, Matsuo O, Matsuno H. Altered behavior in mice with deletion of the alpha2-antiplasmin gene. PLoS One. 2014;9(5):e97947.
  • Plekanchuk VS, Prokudina OI, Ryazanova MA. Social behavior and spatial orientation in rat strains with genetic predisposition to catatonia (GC) and stereotypes (PM). Vavilovskii Zhurnal Genet Selektsii. 2022;26(3):281-9.
  • Cioato SG, Medeiros LF, Marques Filho PR, Vercelino R, de Souza A, Scarabelot VL, et al. Long-lasting effect of transcranial direct current stimulation in the reversal of hyperalgesia and cytokine alterations induced by the neuropathic pain model. Brain Stimul. 2016;9(2):209-17.
  • Santos DS, Lopes BC, Medeiros LF, Assumpção JAF, de Souza A, Salvi AA, et al. Transcranial direct current stimulation (tDCS) induces analgesia in rats with neuropathic pain and alcohol abstinence. Neurochem Res. 2020;45(11):2653-63.
  • Hacısüleyman L, Saraç B, Joha Z. Analgesic effects of vilazodone, indatraline, and talsupram in a rat model of neuropathic pain. Turk J Pharm Sci. 2022;19(3):336-42.
  • Arslan R, Aydin S, Nemutlu Samur D, Bektas N. The possible mechanisms of protocatechuic acid-induced central analgesia. Saudi Pharm J. 2018;26(4):541-5.
  • Zhang Y, Ma S, Ke X, Yi Y, Yu H, Yu D, Li Q, Shang Y, Lu Y, Pei L. The mechanism of Annexin A1 to modulate TRPV1 and nociception in dorsal root ganglion neurons. Cell Biosci. 2021;11(1):167.
  • De la Luz-Cuellar YE, Rodríguez-Palma EJ, Franco-Enzástiga Ú, Déciga-Campos M, Mercado F, Granados-Soto V. Spinal dopaminergic D1 and D5 receptors contribute to reserpine-induced fibromyalgia-like pain in rats. Brain Res. 2023;1799:148167.
  • Girotra P, Singh SK. A comparative study of orally delivered PBCA and ApoE coupled BSA nanoparticles for brain targeting of sumatriptan succinate in therapeutic management of migraine. Pharm Res. 2016;33(7):1682-95.
There are 25 citations in total.

Details

Primary Language English
Subjects Clinical Sciences (Other)
Journal Section Invited Review
Authors

Güven Akçay 0000-0003-3418-8825

Early Pub Date May 31, 2024
Publication Date June 30, 2024
Submission Date March 7, 2024
Acceptance Date May 7, 2024
Published in Issue Year 2024 Volume: 26 Issue: S1

Cite

APA Akçay, G. (2024). Animal Experiments Used in Experimental Neuroscience Research: Learning, Memory, Anxiety, Depression and Motor Function Behavioural Experiments. Duzce Medical Journal, 26(S1), 65-71. https://doi.org/10.18678/dtfd.1493348
AMA Akçay G. Animal Experiments Used in Experimental Neuroscience Research: Learning, Memory, Anxiety, Depression and Motor Function Behavioural Experiments. Duzce Med J. June 2024;26(S1):65-71. doi:10.18678/dtfd.1493348
Chicago Akçay, Güven. “Animal Experiments Used in Experimental Neuroscience Research: Learning, Memory, Anxiety, Depression and Motor Function Behavioural Experiments”. Duzce Medical Journal 26, no. S1 (June 2024): 65-71. https://doi.org/10.18678/dtfd.1493348.
EndNote Akçay G (June 1, 2024) Animal Experiments Used in Experimental Neuroscience Research: Learning, Memory, Anxiety, Depression and Motor Function Behavioural Experiments. Duzce Medical Journal 26 S1 65–71.
IEEE G. Akçay, “Animal Experiments Used in Experimental Neuroscience Research: Learning, Memory, Anxiety, Depression and Motor Function Behavioural Experiments”, Duzce Med J, vol. 26, no. S1, pp. 65–71, 2024, doi: 10.18678/dtfd.1493348.
ISNAD Akçay, Güven. “Animal Experiments Used in Experimental Neuroscience Research: Learning, Memory, Anxiety, Depression and Motor Function Behavioural Experiments”. Duzce Medical Journal 26/S1 (June 2024), 65-71. https://doi.org/10.18678/dtfd.1493348.
JAMA Akçay G. Animal Experiments Used in Experimental Neuroscience Research: Learning, Memory, Anxiety, Depression and Motor Function Behavioural Experiments. Duzce Med J. 2024;26:65–71.
MLA Akçay, Güven. “Animal Experiments Used in Experimental Neuroscience Research: Learning, Memory, Anxiety, Depression and Motor Function Behavioural Experiments”. Duzce Medical Journal, vol. 26, no. S1, 2024, pp. 65-71, doi:10.18678/dtfd.1493348.
Vancouver Akçay G. Animal Experiments Used in Experimental Neuroscience Research: Learning, Memory, Anxiety, Depression and Motor Function Behavioural Experiments. Duzce Med J. 2024;26(S1):65-71.