Troloks ve Neokuproin’in Sıçanlarda Deneysel Hafif Travma Sonucu Beyin Hasarı Üzerindeki Terapötik Etkisi

Year 2024, Volume: 26 Issue: 3, 263 - 268, 30.12.2024

Selma Yaman , Güven Akçay , Fatma Yesilyurt

https://doi.org/10.18678/dtfd.1562334

Abstract

Amaç: Bu çalışmanın amacı hafif travmatik beyin hasarı (hTBH) olan deneysel rat modelinde Troloks ve neokuprin tedavilerinin terapötik etkinliğinin değerlendirilmesidir.
Gereç ve Yöntemler: Kırk rat, kontrol, TBH, Troloks ve neokuprin olarak gruplandırıldı. TBH oluşturmak amacı ile Marmarou Weight Drop Model uygulandıktan sonraki 2. saat diliminde 80 mg/kg/gün Troloks (i.p.) ve 100 μM/gün neokuproin (i.p.) tedavileri yapıldı. Lokomotor aktiviteyi ölçmek için açık alan ve dikkat ve kısa süreli hafıza yeni nesne tanıma testleri gerçekleştirildi. Sıçanların hipokampüs dokularındaki IL-1β, IL-10, TNF-α ve TGF-β düzeyleri ELISA yöntemi ile analiz edildi.
Bulgular: Açık alan ve yeni nesne tanıma test sonuçları, alınan toplam yolun TBH grubunda kontrol grubuna kıyasla önemli ölçüde azaldığını gösterdi (p<0,001). Troloks (p=0,018) ve neokuproin (p=0,002) gruplarında ise TBH’a kıyasla lokomotor fonksiyonlarında anlamlı bir artış gözlendi. Kısa süreli bellek test sonuçları, TBH’da kontrole kıyasla azalırken (p<0,001), Troloks ve neokuproin gruplarında ise TBH’a kıyasla arttı (her iki p<0,001). Kontrole kıyasla TBH grubunun hipokampüs IL-1β (p=0,012) ve TNF-α (p=0,011) seviyeleri artarken, IL-10 (p=0,031) ve TGF-β (p=0,007) seviyeleri azaldı. Troloks ve neokuproin gruplarında TBH’ye kıyasla tüm proinflamatuar sitokin seviyeleri azalırken, antiinflamatuar sitokin seviyeleri arttı.
Sonuç: Elde edilen bulgular, TBH’da Troloks ve neokuproin tedavilerinin proinflamatuar sitokin üretimini azaltarak ve antiinflamatuar sitokin üretimini artırarak kısa süreli bellek ve lokomotor aktivite düzeyinde anlamlı iyileşmeler sağladıklarını göstermiştir.

Keywords

Neokuprin, Troloks, Travmatik beyin hasarı, Lokomotor aktivite, Nöroinflamasyon

Therapeutic Effects of Trolox and Neocuproine on Experimental Mild Traumatic Brain Injury in Rats

Abstract

Aim: This study aimed to evaluate the therapeutic efficacy of Trolox and neocuproine treatment in an experimental rat model of mild traumatic brain injury (mTBI).
Material and Methods: Forty rats were grouped as Control, TBI, Trolox, and neocuproine. After the Marmarou Weight Drop Model was used to create TBI, 80 mg/kg/day Trolox (i.p.), and 100 μM/day Neocuproine (i.p.) treatments were applied in the 2nd hour. Open field, attention, and short-term memory novel object recognition tests were performed to measure locomotor activity. IL-1β, IL-10, TNF-α, and TGF-β levels in the hippocampus tissues of the rats were analyzed by the ELISA method.
Results: Open field and novel object recognition test results showed that the total path traveled was significantly decreased in the TBI group compared to the control group (p<0.001). A significant increase in locomotor functions was observed in the Trolox (p=0.018) and neocuproine (p=0.002) groups compared to TBI. Short-term memory test results decreased in TBI compared to control (p<0.001), while they increased in the Trolox and neocuproine groups compared to TBI (both p<0.001). Hippocampus IL-1β (p=0.012) and TNF-α (p=0.011) levels increased, while IL-10 (p=0.031) and TGF-β (p=0.007) levels decreased compared to control in the TBI group. While all proinflammatory cytokine levels decreased, antiinflammatory cytokine levels increased in the Trolox and neocuproine groups compared to TBI.
Conclusion: The present findings showed that Trolox and neocuproine treatments in TBI provided significant improvements in short-term memory, and locomotor activity levels by reducing proinflammatory cytokine production, and increasing antiinflammatory cytokine production.

Keywords

Neocuproine, Trolox, traumatic brain injury, locomotor activity, neuroinflammation

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