Investigation of Anti-inflammatory, Antioxidative, and Cardioprotective Effects of Combined Metformin and Exercise in Rats

Year 2024, Issue: Early Access

Özge Beyazçiçek , Ersin Beyazçiçek , Ali Gök , Murat Tekbaş , Halit Diril , Şerif Demir

https://doi.org/10.18678/dtfd.1572910

Abstract

Aim: This study aimed to investigate the anti-inflammatory, antioxidative and cardioprotective effects of exercise and metformin treatment applied alone or in combination.
Material and Methods: In this study, 42 male Wistar rats were used. The rats were separated into six groups as control (CONT), exercise (EXE), 100 mg/kg metformin (M100), 200 mg/kg metformin (M200), 100 mg/kg metformin+exercise (M100+EXE), and 200 mg/kg metformin+exercise (M200+EXE). Exercise was applied for 10 weeks including exercise training. Metformin was administered 30 minutes before exercise. At the end of the study, levels of C-reactive protein (CRP), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), interleukin-10 (IL-10), cardiac troponin-I (cTn-I), creatine kinase-muscle/brain (CK-MB), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) and malondialdehyde (MDA) in serum samples from rats were quantified using the ELISA method.
Results: The combined application of metformin and exercise significantly decreased cTn-I, CK-MB, MDA, TNF-α, CRP and IL-6 levels (p<0.001). In contrast, it increased SOD, CAT, GPx, and IL-10 levels significantly (p<0.001). Glucose levels of groups treated alone or in combination were found statistically significantly less than CONT group (p<0.001).
Conclusion: The findings of this study reveal that both metformin and exercise administration, alone or in combination, exert significant anti-inflammatory, antioxidant, and cardioprotective effects in Wistar rats. These results suggest that combining metformin therapy with regular exercise may offer a synergistic approach to reducing cardiovascular risk factors and enhancing antioxidant defenses.

Keywords

Longevity, ardiovascular diseases, hyperglycemia, oxidative stress, metformin, exercise

Sıçanlarda Kombine Metformin ve Egzersizin Anti-inflamatuar, Antioksidatif ve Kardiyoprotektif Etkilerinin Araştırılması

Abstract

Amaç: Bu çalışmanın amacı egzersiz ve metformin tedavisinin tek başına veya birlikte uygulanmasının anti-enflamatuar, antioksidatif ve kardiyoprotektif etkilerinin araştırılmasıdır.
Gereç ve Yöntemler: Bu çalışmada 42 adet erkek Wistar sıçan kullanılmıştır. Sıçanlar kontrol (CONT), egzersiz (EXE), 100 mg/kg metformin (M100), 200 mg/kg metformin (M200), 100 mg/kg metformin+egzersiz (M100+EXE) ve 200 mg/kg metformin+egzersiz (M200+EXE) olmak üzere altı gruba ayrılmıştır. Egzersiz, egzersiz eğitimi de dahil olmak üzere 10 hafta boyunca uygulanmıştır. Metformin egzersizden 30 dakika önce uygulanmıştır. Çalışma sonunda sıçanlardan alınan serum örneklerinde, C-reaktif protein (CRP), tümör nekroz faktörü-alfa (TNF-α), interlökin-6 (IL-6), interlökin-10 (IL-10), kardiyak troponin-I (cTn-I), kreatin kinaz-kas/beyin (CK-MB), katalaz (CAT), süperoksit dismutaz (SOD), glutatyon peroksidaz (GPx) ve malondialdehit (MDA) ELISA yöntemi kullanılarak ölçülmüştür.
Bulgular: Metformin ve egzersizin birlikte uygulanması cTn-I, CK-MB, MDA, TNF-α, CRP ve IL-6 düzeylerini anlamlı şekilde azaltmıştır (p<0,001). Buna karşılık, SOD, CAT, GPx ve IL-10 düzeylerini de önemli şekilde artırmıştır (p<0,001). Tek başına veya kombinasyon halinde tedavi edilen grupların glukoz seviyeleri CONT grubundan istatistiksel olarak anlamlı şekilde daha düşük bulunmuştur (p<0,001).
Sonuç: Bu çalışmanın bulguları, hem metformin hem de egzersiz uygulamasının, tek başına veya kombinasyon halinde, Wistar sıçanlarında önemli anti-inflamatuar, antioksidan ve kardiyoprotektif etkiler gösterdiğini ortaya koymaktadır. Bu sonuçlar, metformin tedavisinin düzenli egzersizle birleştirilmesinin, kardiyovasküler risk faktörlerini azaltmak ve antioksidan savunmaları güçlendirmek için sinerjik bir yaklaşım sunabileceğini düşündürmektedir.

Keywords

Uzun ömür, kardiyovasküler hastalıklar, hiperglisemi, oksidatif stress, metformin, egzersiz

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