Elettaria Cardamomum Ekstraktının Proleukin İlacı Kombinasyonu ile Mide Kanseri Hücre Hattı Üzerindeki İmmünostimulan/Sitotoksik Etkilerinin İncelenmesi

Year 2022, , 283 - 294, 30.12.2022

Yağmur Hamurci , Murat Ihlamur , Yağmur Zengin

https://doi.org/10.55007/dufed.1133501

Cited By: 2

Abstract

Kanser, hücrelerin kontrolsüz çoğalması sonucunda meydana gelmektedir. Farklı kanser türleri olmakla birlikte mide kanseri dünyada en çok görülen kanser türleri arasında yer almaktadır. Mide kanserine karşı birçok tedavi yaklaşımı geliştirilmiştir. Ancak günümüzde kullanılan tedavi yöntemleri, kanser hücrelerini öldürmekle birlikte sağlıklı hücrelere de zarar vermektedir. Kullanılan tedavi yöntemlerinin dezavantajlarından dolayı bitkisel tedavi yaklaşımları kullanılmaya başlanmıştır. Elettaria Cardamomum (E. Cardamomum) (kakule) ekstraktında bulunun Diindolilmetan (DIM) ve indol-3-karbinol (I3C) molekülleri hormon yolaklarını baskılayarak kanser hücrelerini öldürdüğü literatürde belirtilmektedir. Bu çalışmada, E. Cardamomum ve proleukin ilaç kombinasyonlarının AGS, J774, THP-1 hücre hatları üzerindeki etkisi araştırılmıştır. Sonuç olarak, kakule ekstraktında bulunan I3C ve DIM molekülleri sayesinde AGS mide kanseri hücre hattında yüksek sitotoksik etkiye sahip olduğu düşünülmektedir. Kakule ekstraktı ile kombine edilen proleukin ilacının ise AGS hücrelerinde kakule ekstraktının tek başına kullanımına göre daha fazla öldürme oranına sahip olduğu belirlenmiştir.

Keywords

Mide kanseri, AGS, E. Cardamomum, Sitotoksisite

Investigation of Immunostimulant/Cytotoxic Effects of Elettaria Cardamomum Extract with Combination of Proleukin Drug on Gastric Cancer Cell Line

Abstract

Cancer occurs as a result of uncontrolled proliferation of cells. Although there are different types of cancer, gastric cancer is among the most common cancer types in the world. Many treatment approaches have been developed against gastric cancer. However, the treatment methods used today not only kill cancer cells but also damage healthy cells. Due to the disadvantages of the treatment methods used, herbal treatment approaches have started to be used. It is stated in the literature that Diindolylmethane (DIM) and indole-3-carbinol (I3C) molecules in Elettaria Cardamomum (E. Cardamomum) extract kill cancer cells by suppressing hormone pathways. In this study, the effect of E. Cardamomum and proleukin drug combinations on AGS, J774, THP-1 cell lines was investigated. As a result, it was determined that cardamom extract has a high cytotoxic effect on AGS gastric cancer cell line thanks to I3C and DIM molecules. It was determined that proleukin drug combined with cardamom extract had a higher rate of killing in AGS cells than the use of cardamom extract alone.

Keywords

Gastric cancer, AGS, E. Cardamomum, Cytotoxicity

References

• T. A. Baudino, "Targeted Cancer Therapy: The Next Generation of Cancer Treatment", Current Drug Discovery Technologies, vol. 12, no. 1, pp. 3-20, 2015.
• S. S. Joshi, B. D. Badgwell, "Current treatment and recent progress in gastric cancer", CA: A Cancer Journal for Clinicians, vol. 71, no. 3, pp. 264-279, 2021.
• E. C. Smyth, M. Nilsson, H. I. Grabsch, N. C. van Grieken, F. Lordick, "Gastric cancer", Lancet, vol. 396, no. 10251, pp. 635-648, 2020.
• W. Wang, H. S. Luo, "Reversal of chemoresistance to vincristine in gastric cancer cells by NF-kappaB inhibitor", Zhonghua Zhong Liu Za Zhi, vol. 27, no. 6, pp. 335-8, 2005.
• M. Schmidinger, M. Hejna, C. C. Zielinski, "Aldesleukin in advanced renal cell carcinoma", Expert Review of Anticancer Therapy, vol. 4, no. 6, pp. 957-80, 2004.
• C. Sibertin-Blanc, J. Ciccolini, E. Norguet, B. Lacarelle, L. Dahan, J. F. Seitz, "Monoclonal antibodies for treating gastric cancer: promises and pitfalls", Expert Opinion on Biological Therapy, vol. 16, no. 6, pp. 759-69, 2016.
• S. Goyal, N. Gupta, S. Chatterjee, S. Nimesh, "Natural Plant Extracts as Potential Therapeutic Agents for the Treatment of Cancer", Current Topics in Medicinal Chemistry, vol. 17, no. 2, pp. 96-106, 2017.
• G. R. Cárdenas Garza, J. H. Elizondo Luévano, A. F. Bazaldúa Rodríguez, A. Chávez Montes, R. A. Pérez Hernández, A. J. Martínez Delgado, O. E. Rodríguez Luis,’’ enefits of Cardamom (Elettaria cardamomum (L.) Maton) and Turmeric (Curcuma longa L.) Extracts for Their Applications as Natural Anti-Inflammatory Adjuvants’’, Plants, vol.10, no. 9, pp. 1908, 2021.
• S. M. Jump, J. Kung, R. Staub, M. A. Kinseth, E. J. Cram, L. N. Yudina, G. L. Firestone, ‘‘N-Alkoxy derivatization of indole-3-carbinol increases the efficacy of the G1 cell cycle arrest and of I3C-specific regulation of cell cycle gene transcription and activity in human breast cancer cells’’, Biochemical Pharmacology, vol. 75, no. 3, pp. 713-724, 2008.
• Q. W. Zhang, L. G. Lin, W. C. Ye, "Techniques for extraction and isolation of natural products: a comprehensive review", Chinese Medical, vol. 13, pp. 20, 2018.
• M. Ihlamur, B. Akgül, E. S. Abamor, ‘’Farklı hücre hatlarında besiyeri ve FBS’in hücre proliferasyonu üzerindeki etkilerinin incelenmesi’’, Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi, vol. 17, pp. 55-64, 2022.
• I. Guevara, J. Iwanejko, A. Dembińska-Kieć, J. Pankiewicz, A. Wanat, P. Anna, A. Szczudlik, ‘’Determination of nitrite/nitrate in human biological material by the simple Griess reaction’’, Clinica Chimica Acta, vol. 274, no. 2, pp. 177-188, 1998.
• M. Ihlamur, H. Başarı, Y. Zengin E. S. Abamor, ‘Evaluation of ımmunostimulant/cytotoxic activity of human breast cancer prepared by different antigen preparation methods with adjuvants combination’’, Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi, vol. 17, pp. 96-110, 2022.
• G. M. Cragg, J. M. Pezzuto, "Natural Products as a Vital Source for the Discovery of Cancer Chemotherapeutic and Chemopreventive Agents", Medical principles and practice: international journal of the Kuwait University, Health Science Centre, vol. 25, Suppl 2, pp. 41-59, 2016.
• B. B. Aggarwal, H. Ichikawa, "Molecular targets and anticancer potential of indole-3-carbinol and its derivatives", Cell Cycle, vol. 4, no. 9, pp. 1201-15, 2005.
• S. Qiblawi, A. Kausar, S. Shahid, M. Saeed, A. Alazzeh, "Therapeutic Interventions of Cardamom in Cancer and Other Human Diseases", Journal of Pharmaceutical Research International, vol. 32, pp. 74-78, 2020.
• C. A. Thomson, E. Ho, M. B. Strom, "Chemopreventive properties of 3,3'-diindolylmethane in breast cancer: evidence from experimental and human studies", Nutrition reviews, vol. 74, no. 7, pp. 432-443, 2016.