Asetonla Muamele Edilmiş Docetaxelin PC3 ve PNT1A Hücreleri Üzerindeki Sitotoksik Etkisi

Muhammed Güngören; Ersin Kılınç; Zübeyde Baysal

EN TR

Cytotoxic Effect of Acetone-Treated Docetaxel on PC3 and PNT1A Cells

Abstract

In this study, the cytotoxic effects of docetaxel, which is used as an important chemotherapeutic agent in the treatment of prostate cancer, on human intact prostate cell (PNT1A) and human cancerous prostate cell (PC3) were investigated. In the study, pure and acetone-soaked form of commercial docetaxel was used. It was determined that there was no significant difference between the use of different forms of docetaxel in the analyzes performed after 24, 48 and 72 hours of incubation using the MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide] test (p<0.01). It was found that docetaxel forms have dose dependent cytotoxic effects on PC3 and PNT1A cells. It was determined that the dose dependence of the cytotoxic effect was quite high, especially in the first 24 hours (p<0.05 for PC3, p<0.01 for PNT1A). The IC50 value of pure docetaxel for PC3 cell was calculated as 0.0116 μg/mL and for PNT1A cell as 3.5462 μg/mL at 48. h. These values show that docetaxel produces a cytotoxic effect in PNT1A cells, although it is lower than in PC3 cells. Using docetaxel, which has therapeutic effects in resistant prostate cancer cells, together with drug delivery systems is considered as an appropriate approach for reducing the damage in healthy cells.

Keywords

Docetaxel, MIT assay, Prostate cancer, Cytotoxicity-dose correlation

Asetonla Muamele Edilmiş Docetaxelin PC3 ve PNT1A Hücreleri Üzerindeki Sitotoksik Etkisi

Abstract

Bu çalışmada, prostat kanserinin tedavisinde önemli bir kemoterapötik ajan olarak kullanılan docetaxelin, insan sağlam prostat hücresi (PNT1A) ve insan kanserli prostat hücresi (PC3) üzerindeki sitotoksik etkileri araştırılmıştır. Çalışmada ticari docetaxelin saf ve asetonda bekletilmiş formu kullanılmıştır. MTT [3-(4,5-dimetiltiazol-2-il)-2,5-difenil-2H-tetrazolyum bromid] testi kullanılarak 24, 48 ve 72 saatlik inkübasyon sonrası yapılan analizlerde farklı docetaxel formlarının kullanımı arasında anlamlı bir fark bulunmadığı tespit edilmiştir (p<0.01). Elde edilen bulgular, docetaxel formlarının PC3 ve PNT1A hücreleri üzerinde doza bağımlı sitotoksik etkilerinin varlığını ortaya koymaktadır. Sitotoksik etkinin doza bağımlılığının özellikle ilk 24 saatte oldukça yüksek olduğu belirlenmiştir (PC3 için p<0.05, PNT1A için p<0.01). 48. saatte saf docetaxelin PC3 hücresi için IC50 değeri 0.0116 μg/mL, PNT1A hücresi için IC50 değeri 3.5462 μg/mL olarak hesaplanmıştır. Bu değerler docetaxelin PNT1A hücrelerinde, PC3 hücrelerine kıyasla daha düşük olsada, sitotoksik etki oluşturduğunu göstermektedir. Direnç geliştirmiş prostat kanseri hücrelerinde de terapötik etkileri olan docetaxelin ilaç taşıma sistemleri ile beraber kullanılarak sağlam hücrelerdeki hasarının azaltılması uygun bir yaklaşım olarak düşünülmektedir.

Keywords

Docetaxel, MTT testi, Prostat kanseri, Sitotoksisite-doz ilişkisi

Supporting Institution

Dicle Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi Koordinatörlüğü

Project Number

Proje Numarası: TEKNİK-MYO-19-001 ve FEN.19.015

Thanks

Bu araştırma, Dicle Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi Koordinatörlüğü’nce desteklenmiştir (Proje Numarası: TEKNİK-MYO-19-001 ve FEN.19.015).

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Details

Primary Language

Turkish

Subjects

-

Journal Section

Research Article

Authors

Muhammed Güngören ^*
0000-0002-3122-7086
Türkiye

Ersin Kılınç
0000-0001-5223-9919
Türkiye

Zübeyde Baysal
0000-0001-7682-4469
Türkiye

Publication Date

December 30, 2021

Submission Date

June 18, 2021

Acceptance Date

June 25, 2021

Published in Issue

Year 2021 Volume: 10 Number: 2

IZ

https://izlik.org/JA55NA69FK

APA

Güngören, M., Kılınç, E., & Baysal, Z. (2021). Asetonla Muamele Edilmiş Docetaxelin PC3 ve PNT1A Hücreleri Üzerindeki Sitotoksik Etkisi. Dicle Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 10(2), 109-120. https://izlik.org/JA55NA69FK

AMA

1.Güngören M, Kılınç E, Baysal Z. Asetonla Muamele Edilmiş Docetaxelin PC3 ve PNT1A Hücreleri Üzerindeki Sitotoksik Etkisi. DUFED. 2021;10(2):109-120. https://izlik.org/JA55NA69FK

Chicago

Güngören, Muhammed, Ersin Kılınç, and Zübeyde Baysal. 2021. “Asetonla Muamele Edilmiş Docetaxelin PC3 Ve PNT1A Hücreleri Üzerindeki Sitotoksik Etkisi”. Dicle Üniversitesi Fen Bilimleri Enstitüsü Dergisi 10 (2): 109-20. https://izlik.org/JA55NA69FK.

EndNote

Güngören M, Kılınç E, Baysal Z (December 1, 2021) Asetonla Muamele Edilmiş Docetaxelin PC3 ve PNT1A Hücreleri Üzerindeki Sitotoksik Etkisi. Dicle Üniversitesi Fen Bilimleri Enstitüsü Dergisi 10 2 109–120.

IEEE

[1]M. Güngören, E. Kılınç, and Z. Baysal, “Asetonla Muamele Edilmiş Docetaxelin PC3 ve PNT1A Hücreleri Üzerindeki Sitotoksik Etkisi”, DUFED, vol. 10, no. 2, pp. 109–120, Dec. 2021, [Online]. Available: https://izlik.org/JA55NA69FK

ISNAD

Güngören, Muhammed - Kılınç, Ersin - Baysal, Zübeyde. “Asetonla Muamele Edilmiş Docetaxelin PC3 Ve PNT1A Hücreleri Üzerindeki Sitotoksik Etkisi”. Dicle Üniversitesi Fen Bilimleri Enstitüsü Dergisi 10/2 (December 1, 2021): 109-120. https://izlik.org/JA55NA69FK.

JAMA

1.Güngören M, Kılınç E, Baysal Z. Asetonla Muamele Edilmiş Docetaxelin PC3 ve PNT1A Hücreleri Üzerindeki Sitotoksik Etkisi. DUFED. 2021;10:109–120.

MLA

Güngören, Muhammed, et al. “Asetonla Muamele Edilmiş Docetaxelin PC3 Ve PNT1A Hücreleri Üzerindeki Sitotoksik Etkisi”. Dicle Üniversitesi Fen Bilimleri Enstitüsü Dergisi, vol. 10, no. 2, Dec. 2021, pp. 109-20, https://izlik.org/JA55NA69FK.

Vancouver

1.Muhammed Güngören, Ersin Kılınç, Zübeyde Baysal. Asetonla Muamele Edilmiş Docetaxelin PC3 ve PNT1A Hücreleri Üzerindeki Sitotoksik Etkisi. DUFED [Internet]. 2021 Dec. 1;10(2):109-20. Available from: https://izlik.org/JA55NA69FK

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https://doi.org/10.55007/dufed.xxxxxxx

Cytotoxic Effect of Acetone-Treated Docetaxel on PC3 and PNT1A Cells

Abstract

Keywords

Asetonla Muamele Edilmiş Docetaxelin PC3 ve PNT1A Hücreleri Üzerindeki Sitotoksik Etkisi

Abstract

Keywords

Supporting Institution

Project Number

Thanks

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

IZ

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