Organotipik Beyin Kesitleri Kullanımının Nörobiyolojik Çalışmalardaki Yeri

Year 2021, Volume: 10 Issue: 1, 95 - 107, 10.06.2021

Elif Mutlu , Hasan H.s. Abuıyada

Abstract

Organotipik beyin kesit kültürleri günümüzde nörobiyoloji araştırmalarında rutin bir protokol haline gelmiştir. Beyin kesit kültürü tekniklerindeki ilerleme sayesinde birçok beyin hastalığı patofizyolojisinin in vivo duruma çok yakın bir şekilde doku bağlamında incelenmesi fırsatı doğmuştur. In vivo çalışmalarda anesteziklerin ve kas gevşetici maddelerin uzun süreli kullanımına alternatif olması yanında hücre kültürlerinin ve homojenatlarının aksine yapısal bütünlüğünü sürdürebilmesi gibi avantajlar sunarken bu kültür ortamı bazı dezavantajları da beraberinde getirmektedir. Bu derleme çalışması boyunca beyin kesit kültürü teknolojisi avantajları ve dezavantajları ile birlikte ele alınarak nörobiyoloji çalışmalarındaki yeri incelenmiştir. Hücresel bütünlüğün korunduğu bu sistemlerin nörodejenerasyon, nörogenez, nörotoksisite gibi birçok alanda in vitro hücre kültürü ve in vivo deney hayvanı çalışmalarına kıyasla çok daha verimli ve kullanışlıdır.

Keywords

Beyin, Kesit kültürü, Organotipik beyin kesiti, Nörobiyoloji

Use of Organotypic Brain Slices in Neurobiological Studies

Abstract

Organotypic brain slice cultures have now become a routine protocol in neurobiology researches. Thanks to the advancement in brain section culture techniques, the opportunity has emerged to examine the pathophysiology of many brain diseases in a tissue context very close to the in vivo situation. In addition to being an alternative to the long-term use of anesthetics and muscle relaxants in in vivo studies, it offers advantages such as maintaining the structural integrity of cell cultures and homogenates, while this culture environment also brings some disadvantages. Throughout this review study, brain slice culture technology was considered together with its advantages and disadvantages and its place in neurobiology studies was examined. These systems, in which cellular integrity is preserved, are much more efficient and useful in many areas such as neurodegeneration, neurogenesis, neurotoxicity compared to in vitro cell culture and in vivo experimental animal studies.

Keywords

Brain, Slice culture, Organotypic brain slice, Neurobiology

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