Evaluation of Cytotoxicity of Various Materials Using For Treatment of Tooth Perforations

Abstract

The cytotoxic effects of some perforation repair materials as BA, MTA, Super EBA and amalgam was evaluated on L-929 cells with the MTT assay n the first part of this study, 5x2 mm cylin- ders of each material samples were prepared and placed in DMEM for 24, 48 and 72 hours. 100 μl of eluate was transferred to a 96-well plate contai- ning L-929 cell suspension. After 24 hours incu- bation the extracts were removed from the wells, MTT solution was added to each well and kept in a dark environment for 4 hours. Subsequently, the spectrophotometric absorbance was measured at 570 nm using a spectrophotometer. According to the results of MTT assay, there was no statistically significant difference between BA and MTA in the rates of cell viability at 24- hour evaluation. These two groups showed better results than Super EBA and amalgam. At 48 and 72-hour evaluation of the groups, BA group showed higher rate of cell viability than the other groups, on the other hand the lowest rate of viabi- lity was in the Super EBA group.

Keywords

• Amalgam, BA, MTA, Super EBA, Cytotoxicity

Perforasyon tedavisinde kullanılan çeşitli materyallerin sitotoksisitelerinin değerlendirilmesi

Abstract

Bu çalışmada diş perforasyonlarının tedavisinde kullanılan BA, MTA, Super EBA ve amalgamın L-929 hücre kültüründe MTT testi ile sitotoksik etkileri değerlendirilmiştir. Her bir materyalden 5 mm iç çapında ve 2 mm derinliğinde hazırlanan 15 adet örnek, hücre kültürü plakalarında, üzerine DMEM ilave edilerek 24, 48 ve 72 saat bekletilmiştir. Elde edilen ekstraksiyon sıvıları, L-929 hücre süspansiyonu içeren 96 gözlü hücre kültürü plakalarının her bir gözüne 100 µl yerleştirilmiştir. 24 saatlik inkübasyon süresi sonunda tabletlerden ekstraksiyon sıvıları uzaklaştırılmış ve her bir göze MTT solüsyonu ilave edilerek karanlık bir ortamda 4 saat süre ile bekletilmiştir. Bu sürenin sonunda spektrofotometrede 570 nm’de absorbansları ölçülmüştür. MTT testi sonuçlarına göre 24 saatlik değerlendirmede BA ile MTA grupları arasında hücre canlılık oranları açısından istatistiksel olarak anlamlı bir fark gözlenmezken bu iki grubun Super EBA ve amalgam gruplarından daha iyi sonuçlar verdiği gözlenmiştir. Grupların 48 ve 72 saatlik değerlendirmelerinde BA grubu diğer gruplara göre istatistiksel olarak anlamlı ölçüde daha yüksek canlılık oranı gösterirken, bu zaman noktalarında en düşük canlılık oranının Super EBA grubunda olduğu belirlenmiştir.

Keywords

• Amalgam, BA, MTA, Süper EBA, Sitotoksisite

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