Evaluation of Bacterial Leakage of Retrograde Filling Materials using various Retrograde Cavity Preparation Methods

Abstract

In our study, 140 single root human tooth were used. In order to ensure the root length standardization of the teeth, 15 mm was cut from the apex. One hundred and twenty teeth were randomly divided into three groups each of which included 40 teeth. The 20 teeth left were used as positive n=10 and negative n=10 control groups. Then the root canals were prepared by ProTaper instruments and obturated with lateral condensation. Apical resection of all groups were performed by conventional fissur diamond bur. Retrograde cavity preparation was performed by burr, ultrasonic retrotip and Er:YAG laser in the first, second and third groups, respectively. These main three groups were divided into two subgroups for retrograde cavity filling procedures. These groups were filled using MTA and DiaRoot BioAggregate.. The samples were sterilized with oxide gas for 12 hours. An experiment materials was prepared for bacterial leakage test and sterilized with ethylene oxide gas. E. faecalis and S. aureus inoculum was injected into sterile Eppendorf tubes. The samples were evaluated by bacterial leakage method. Tubes were left to incubation for 90 days in an incubator under the temperature of 35 ± 2 °C. During incubation, E. faecalis and S. aureus in the Eppendorf tube were renewed every five days and every day, glass tubes were controlled and the blurring of BHI culture was recorded as leakage day. The blurring of BHI culture in glass tubes was noted as the leakage and the proliferation of E. faecalis and S. aureus. It was evaluated whether there is a significant difference among the groups in terms of leakage speed and average leakage times, by using log-rank test and with the analysis of Kaplan Meier survival. The value of p

Keywords

• Bacterial leakage, apical resection, retrograde cavity, Er:YAG laser, ultrasonic

FARKLI YÖNTEMLERLE AÇILAN RETROGRAD KAVİTELERDE RETROGRAD DOLGU MADDELERİNİN BAKTERİYEL SIZINTILARININ DEĞERLENDİRİLMESİ

Abstract

Çalışmamızda, 140 adet tek köklü insan dişleri kullanıldı. Dişlerin kök boyu standardizasyonunu sağlamak için apeksten 15 mm uzaklıkta olacak şekilde kesildi. Yüz kırk adet dişin 120 tanesi her bir grupta 40 diş içerecek şekilde rastgele 3 gruba ayrıldı. Geriye kalan 20 dişten ise pozitif n=10 ve negatif n=10 kontrol grubu oluşturuldu. Kök kanalları ProTaper ile prepare edildikten sonra kanallar lateral kondenzasyon yöntemi ile dolduruldu. Tüm grupların apikal rezeksiyonları konvansiyonel elmas fissür frez ile yapıldı. Retrograd kavite preparasyonu, ilk grupta frez, ikinci grupta ultrasonik uç, üçüncü grupta ise Er:YAG lazer ile yapıldı. Bu ana gruplar, retrograd dolguları yapılmak üzere içlerinde iki subgruba ayrıldı. Gruplar MTA ve DiaRoot BioAggregate ile dolduruldu. Hazırlanan örnekler etilen oksit gazı ile steril edildi. Bakteriyel sızıntı deneyi için test düzeneği hazırlandı. E. faecalis ve S. aureus inokulumu, steril Eppendorf tüplerin içine enjekte edildi. Tüpler etüvde 35 ± 2 ºC’ de, 90 gün boyunca inkübasyona bırakıldı. İnkübasyon süresince Eppendorf tüpü içindeki E. faecalis ve S. aureus inokulumu 5 günde bir yenilendi. Cam tüp içerisindeki BHI buyyon’un bulanıklaşması E. faecalis’in ve S. aureus sızıntısı ve üremesi olarak değerlendirildi. Çalışma grupları arasında sızıntı hızlarında ve ortalama sızıntı zamanlarında anlamlı farklılık olup olmadığı Log-Rank testi kullanılarak Kaplan Meier sağkalım analizi ile değerlendirildi. p

Keywords

• Bacterial leakage, apical resection, retrograde cavity, Er:YAG laser, ultrasonic

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