Prevalance of Tannerella Forsythia in Primary Root Canal Infections by 16S rRNA Based PCR

Abstract

The purpose of this study was to assess the prevalence of Tannerella forsythia in the primary infections of root canals and its association with different forms of periradicular diseases using Polymerase Chain Reaction PCR . Samples were collected from acute and chronic forms of periradicular diseases and abscesses. DNA extraction was performed using spin column DNA isolation kit and 16S rRNA gene based PCR analysis was performed. Chi-squared test was used for data analysis. Tannerella forsythia DNA was detected in 18 31,3% of the total 58 samples, in 13 of 26 cases 50% with acute apical periodontitis, three of seven cases 42,8% with acute periradicular abscesses, and 2 of 25 cases 8% with chronic asymptomatic periradicular lesions. It was found to be significantly related with acute apical periodontitis group p=0.002 . T. forsythia must be considered as a potential endodontic pathogen associated with acute symptoms of periradicular disease.

Keywords

• Infection, PCR, root canal, rRNA, Tannerella forsythia

Primer kok kanal enfeksiyonlarında Tannerella forsythia prevalansının 16S rRNA PCR ile incelenmesi

Abstract

Bu çalışmanın amacı Tannerella forsythia’nın primer kök kanal enfeksiyonlarındaki prevalansını ve bu bakterinin değişik formlardaki periradiküler hastalıklar ile ilişkisini Polimeraz Zincir Reaksiyonu PCR yöntemi ile incelemektir. Örnekler, akut ve kronik formlardaki periradiküler hastalıklar ve abselerden elde edildi. Filtreli kolon DNA izolasyon kiti kullanılarak DNA ekstraksiyonu yapıldı ve 16S rRNA gen bazlı PCR yürütüldü. Verilerin istatistiksel analizinde Ki - kare testi kullanıldı. Akut apikal periodontitisli 26 vakanın 13’ünde %50 , akut periradiküler abseli 7 vakanın 3’ünde %42,8 ve kronik asemptomatik periradiküler lezyonlu 25 vakanın 2’sinde %8 olmak üzere toplam 58 vakanın 18’inde %31,3 Tannerella forsythia DNA’sı saptandı. Bakteri varlığı ile akut apikal periodontitis grubu arasındaki ilişki istatistiksel olarak önemli bulundu P=0.002 . Tannerella forsythia periradiküler hasarın akut semptomları ile ilişkili potensiyel bir endodontik patojen olarak düşünülmelidir.

Keywords

• Enfeksiyon, PCR, kök kanalı, rRNA, Tannerella forsythia

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