Correlation between Erythrocyte Membrane Na+K+ATPase and Plasma Homocysteine Levels in Type 2 Diabetes Patient

Abstract

Diabetes Mellitus is the most common of the serious metabolic diseases. It is characterized by metabolic abnormalities; by long-term complication. Correlation between erythrocyte membrane Na⁺K⁺ATPase and plasma homocysteine levels in patient. This study was conducted in 29 patients with Type II Diabetes Mellitus and 15 healthy controls. Erythrocyte membrane Na⁺K⁺ATPase activity and malondialdehyde were measured spectrophotometrically. Plasma homocysteine levels were measured with High Performance Liquid Chromatography method. When compared to the control group, mean plasma homocysteine levels were found to be increased (p<0.001) and mean Na⁺K⁺ATPase values of erythrocyte membranes activities were found to be decreased (p<0.05). There was a negative correlation between erythrocyte membrane Na⁺K⁺ATPase and plasma homocysteine levels in patient group (p< 0,01; r= - 0,49), but any correlation was not detected  in the control group. Malondialdehyde was measurement in the tip 2 diabetic patients decreased more than control group. We found an increased plasma homocysteine levels and decreased erythrocyte membranes Na⁺K⁺ATPase activities in Diabetes Mellitus group. We think that the decrease in activities of Na⁺K⁺ATPase may be due to the increased plasma homocysteine levels.  It was concluded that to be more impact in diabetes which is a complicated disease, cell membrane lipids of oxidation with starting negative the table as a result of high homocysteine levels and reduced antioxidant capacity.

Keywords

• Diabetes Mellitus, Na+K+ATPase, Homocysteine, Malondialdehyde

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